DOCK9 (NM_001130048) Human Tagged ORF Clone
CAT#: RC226443
- TrueORF®
DOCK9 (Myc-DDK-tagged)-Human dedicator of cytokinesis 9 (DOCK9), transcript variant 2
ORF Plasmid: tGFP
"NM_001130048" in other vectors (2)
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CNY 18,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ZIZ1; ZIZIMIN1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC226443 representing NM_001130048
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCTGATAAATGCAGGACAAGTAGTAGAAGTGTCAAAAAGGAACTGGTGATTGAGTCCCCCCTGC AATACAAGGATGCAGCTCAGGGCGAAGTGGAAGCAGAGAGCCCGGGCCCTGTGCCGGCAAAGCCAAAGCT AATTGAGCCACTCGACTATGAAAATGTCATCGTCCAGAAGAAGACTCAGATCCTGAACGACTGTTTACGG GAGATGCTGCTCTTCCCTTACGATGACTTTCAGACGGCCATCCTGAGACGACAGGGTCGATACATATGCT CAACAGTGCCTGCGAAGGCGGAAGAGGAAGCACAGAGCTTGTTTGTTACAGAGTGCATCAAAACCTATAA CTCTGACTGGCATCTTGTGAACTATAAATATGAAGATTACTCAGGAGAGTTTCGACAGCTTCCGAACAAA GTGGTCAAGTTGGATAAACTTCCAGTTCATGTCTATGAAGTTGACGAGGAGGTCGACAAAGATGAGGATG CTGCCTCCCTTGGTTCCCAGAAGGGTGGGATCACCAAGCATGGCTGGCTGTACAAAGGCAACATGAACAG TGCCATCAGCGTGACCATGAGGTCATTTAAGAGACGATTTTTCCACCTGATTCAACTTGGCGATGGATCC TATAATTTGAATTTTTATAAAGATGAAAAGATCTCCAAAGAACCAAAAGGATCAATATTTCTGGATTCCT GTATGGGTGTCGTTCAGAACAACAAAGTCAGGCGTTTTGCTTTTGAGCTCAAGATGCAGGACAAAAGTAG TTATCTCTTGGCAGCAGACAGTGAAGTGGAAATGGAAGAATGGATCACAATTCTAAATAAGATCCTCCAG CTCAACTTTGAAGCTGCAATGCAAGAAAAGCGAAATGGCGACTCTCACGAAGATGATGAACAAAGCAAAT TGGAAGGTTCTGGTTCCGGTTTAGATAGCTACCTGCCGGAACTTGCCAAGAGTGCAAGAGAAGCAGAAAT CAAACTGAAAAGTGAAAGCAGAGTCAAACTTTTTTATTTGGACCCAGATGCCCAGAAGCTTGACTTCTCA TCAGCTGAGCCAGAAGTGAAGTCATTTGAAGAGAAGTTTGGAAAAAGGATCCTTGTCAAGTGCAATGATT TATCTTTCAATTTGCAATGCTGTGTTGCCGAAAATGAAGAAGGACCCACTACAAATGTTGAACCTTTCTT TGTTACTCTATCCCTGTTTGACATAAAATACAACCGGAAGATTTCTGCCGATTTCCACGTAGACCTGAAC CATTTCTCAGTGAGGCAAATGCTCGCCACCACGTCCCCGGCGCTGATGAATGGCAGTGGGCAGAGCCCAT CTGTCCTCAAGGGCATCCTTCATGAAGCCGCCATGCAGTATCCGAAGCAGGGAATATTTTCAGTCACTTG TCCTCATCCAGATATATTTCTTGTGGCCAGAATTGAAAAAGTCCTTCAGGGGAGCATCACACATTGCGCT GAGCCATATATGAAAAGTTCAGACTCTTCTAAGGTGGCCCAGAAGGTGCTGAAGAATGCCAAGCAGGCAT GCCAAAGACTAGGACAGTATAGAATGCCATTTGCTTGGGCAGCAAGGACATTGTTTAAGGATGCATCTGG AAATCTTGACAAAAATGCCAGATTTTCTGCCATCTACAGGCAAGACAGCAATAAGCTATCCAATGATGAC ATGCTCAAGTTACTTGCAGACTTTCGGAAACCTGAGAAGATGGCTAAGCTCCCAGTGATTTTAGGCAATC TAGACATTACAATTGATAATGTTTCCTCAGACTTCCCTAATTATGTTAATTCATCATACATTCCCACAAA ACAATTTGAAACCTGCAGTAAAACTCCCATCACGTTTGAAGTGGAGGAATTTGTGCCCTGCATACCAAAA CACACTCAGCCTTACACCATCTACACCAATCACCTTTACGTTTATCCTAAGTACTTGAAATACGACAGTC AGAAGTCTTTTGCCAAGGCTAGAAATATTGCGATTTGCATTGAATTCAAAGATTCAGATGAGGAAGACTC TCAGCCCCTTAAGTGCATTTATGGCAGACCTGGTGGGCCAGTTTTCACAAGAAGCGCCTTTGCTGCAGTT TTACACCATCACCAAAACCCAGAATTTTATGATGAGATTAAAATAGAGTTGCCCACTCAGCTGCATGAAA AGCACCACCTGTTGCTCACATTCTTCCATGTCAGCTGTGACAACTCAAGTAAAGGAAGCACGAAGAAGAG GGATGTCGTTGAAACCCAAGTTGGCTACTCCTGGCTTCCCCTCCTGAAAGACGGAAGGGTGGTGACAAGC GAGCAGCACATCCCGGTCTCGGCGAACCTTCCTTCGGGCTATCTTGGCTACCAGGAGCTTGGGATGGGCA GGCATTATGGTCCGGAAATTAAATGGGTAGATGGAGGCAAGCCACTGCTGAAAATTTCCACTCATCTGGT TTCTACAGTGTATACTCAGGATCAGCATTTACATAATTTTTTCCAGTACTGTCAGAAAACCGAATCTGGA GCCCAAGCCTTAGGAAACGAACTTGTAAAGTACCTTAAGAGTCTGCATGCGATGGAAGGCCACGTGATGA TCGCCTTCTTGCCCACTATCCTAAACCAGCTGTTCCGAGTCCTCACCAGAGCCACACAGGAAGAAGTCGC GGTTAACGTGACTCGGGTCATTATTCATGTGGTTGCCCAGTGCCATGAGGAAGGATTGGAGAGCCACTTG AGGTCATATGTTAAGTACGCGTATAAGGCTGAGCCATATGTTGCCTCTGAATACAAGACAGTGCATGAAG AACTGACCAAATCCATGACCACGATTCTCAAGCCTTCTGCCGATTTCCTCACCAGCAACAAACTACTGAA GTACTCATGGTTTTTCTTTGATGTACTGATCAAATCTATGGCTCAGCATTTGATAGAGAACTCCAAAGTT AAGTTGCTGCGAAACCAGAGATTTCCTGCATCCTATCATCATGCAGTGGAAACCGTTGTAAATATGCTGA TGCCACACATCACTCAGAAGTTTCGAGATAATCCAGAGGCATCTAAGAACGCGAATCATAGCCTTGCTGT CTTCATCAAGAGATGTTTCACCTTCATGGACAGGGGCTTTGTCTTCAAGCAGATCAACAACTACATTAGC TGTTTTGCTCCTGGAGACCCAAAGACCCTCTTTGAATACAAGTTTGAATTTCTCCGTGTAGTGTGCAACC ATGAACATTATATTCCGTTGAACTTACCAATGCCATTTGGAAAAGGCAGGATTCAAAGATACCAAGACCT CCAGCTTGACTACTCATTAACAGATGAGTTCTGCAGAAACCACTTCTTGGTGGGACTGTTACTGAGGGAG GTGGGGACAGCCCTCCAGGAGTTCCGGGAGGTCCGTCTGATCGCCATCAGTGTGCTCAAGAACCTGCTGA TAAAGCATTCTTTTGATGACAGATATGCTTCAAGGAGCCATCAGGCAAGGATAGCCACCCTCTACCTGCC TCTGTTTGGTCTGCTGATTGAAAACGTCCAGCGGATCAATGTGAGGGATGTGTCACCCTTCCCTGTGAAC GCGGGCATGACTGTGAAGGATGAATCCCTGGCTCTACCAGCTGTGAATCCGCTGGTGACGCCGCAGAAGG GAAGCACCCTGGACAACAGCCTGCACAAGGACCTGCTGGGCGCCATCTCCGGCATTGCTTCTCCATATAC AACCTCAACTCCAAACATCAACAGTGTGAGAAATGCTGATTCGAGAGGATCTCTCATAAGCACAGATTCG GGTAACAGCCTTCCAGAAAGGAATAGTGAGAAGAGCAATTCCCTGGATAAGCACCAACAAAGTAGCACAT TGGGAAATTCCGTGGTTCGCTGTGATAAACTTGACCAGTCTGAGATTAAGAGCCTACTGATGTGTTTCCT CTACATCTTAAAGAGCATGTCTGATGATGCTTTGTTTACATATTGGAACAAGGCTTCAACATCTGAACTT ATGGATTTTTTTACAATATCTGAAGTCTGCCTGCACCAGTTCCAGTACATGGGGAAGCGATACATAGCCA GAACAGGAATGATGCATGCCAGATTGCAGCAGCTGGGCAGCCTGGATAACTCTCTCACTTTTAACCACAG CTATGGCCACTCGGACGCAGATGTTCTGCACCAGTCATTACTTGAAGCCAACATTGCTACTGAGGTTTGC CTGACAGCTCTGGACACGCTTTCTCTATTTACATTGGCGTTTAAGAACCAGCTCCTGGCCGACCATGGAC ATAATCCTCTCATGAAAAAAGTTTTTGATGTCTACCTGTGTTTTCTTCAAAAACATCAGTCTGAAACGGC TTTAAAAAATGTCTTCACTGCCTTAAGGTCCTTAATTTATAAGTTTCCCTCAACATTCTATGAAGGGAGA GCGGACATGTGTGCGGCTCTGTGTTACGAGATTCTCAAGTGCTGTAACTCCAAGCTGAGCTCCATCAGGA CGGAGGCCTCCCAGCTGCTCTACTTCCTGATGAGGAACAACTTTGATTACACTGGAAAGAAGTCCTTTGT CCGGACACATTTGCAAGTCATCATATCTGTCAGCCAGCTGATAGCAGACGTTGTTGGCATTGGGGGAACC AGATTCCAGCAGTCCCTGTCCATCATCAACAACTGTGCCAACAGTGACCGGCTTATTAAGCACACCAGCT TCTCCTCTGATGTGAAGGACTTAACCAAAAGGATACGCACGGTGCTAATGGCCACCGCCCAGATGAAGGA GCATGAGAACGACCCAGAGATGCTGGTGGACCTCCAGTACAGCCTGGCCAAATCCTATGCCAGCACGCCC GAGCTCAGGAAGACGTGGCTCGACAGCATGGCCAGGATCCATGTCAAAAATGGCGATCTCTCAGAGGCAG CAATGTGCTATGTCCACGTAACAGCCCTAGTGGCAGAATATCTCACACGGAAAGAAGCAGTCCAGTGGGA GCCGCCCCTTCTCCCCCACAGCCATAGCGCCTGCCTGAGGAGGAGCCGGGGAGGCGTGTTTAGACAAGGA TGCACCGCCTTCAGGGTCATTACCCCAAACATCGACGAGGAGGCCTCCATGATGGAAGACGTGGGGATGC AGGATGTCCATTTCAACGAGGATGTGCTGATGGAGCTCCTTGAGCAGTGCGCAGATGGACTCTGGAAAGC CGAGCGCTACGAGCTCATCGCCGACATCTACAAACTTATCATCCCCATTTATGAGAAGCGGAGGGATTTT GAGAGGCTGGCCCATCTGTATGACACGCTGCACCGGGCCTACAGCAAAGTGACCGAGGTCATGCACTCGG GCCGCAGGCTTCTGGGGACCTACTTCCGGGTAGCCTTCTTCGGGCAGGCAGCGCAATACCAGTTTACAGA CAGTGAAACAGATGTGGAGGGATTCTTTGAAGATGAAGATGGAAAGGAGTATATTTACAAGGAACCCAAA CTCACACCGCTGTCGGAAATTTCTCAGAGACTCCTTAAACTGTACTCGGATAAATTTGGTTCTGAAAATG TCAAAATGATACAGGATTCTGGCAAGGTCAACCCTAAGGATCTGGATTCTAAGTATGCATACATCCAGGT GACTCACGTCATCCCCTTCTTTGACGAAAAAGAGTTGCAAGAAAGGAAAACAGAGTTTGAGAGATCCCAC AACATCCGCCGCTTCATGTTTGAGATGCCATTTACGCAGACCGGGAAGAGGCAGGGCGGGGTGGAAGAGC AGTGCAAACGGCGCACCATCCTGACAGCCATACACTGCTTCCCTTATGTGAAGAAGCGCATCCCTGTCAT GTACCAGCACCACACTGACCTGAACCCCATCGAGGTGGCCATTGACGAGATGAGTAAGAAGGTGGCGGAG CTCCGGCAGCTGTGCTCCTCGGCCGAGGTGGACATGATCAAACTGCAGCTCAAACTCCAGGGCAGCGTGA GTGTTCAGGTCAATGCTGGCCCACTAGCATATGCGCGAGCTTTCTTAGATGATACAAACACAAAGCGATA TCCTGACAATAAAGTGAAGCTGCTTAAGGAAGTTTTCAGGCAATTTGTGGAAGCTTGCGGTCAAGCCTTA GCGGTAAACGAACGTCTGATTAAAGAAGACCAGCTCGAGTATCAGGAAGAAATGAAAGCCAACTACAGGG AAATGGCGAAGGAGCTTTCTGAAATCATGCATGAGCAGCTGGGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC226443 representing NM_001130048
Red=Cloning site Green=Tags(s) MQADKCRTSSRSVKKELVIESPLQYKDAAQGEVEAESPGPVPAKPKLIEPLDYENVIVQKKTQILNDCLR EMLLFPYDDFQTAILRRQGRYICSTVPAKAEEEAQSLFVTECIKTYNSDWHLVNYKYEDYSGEFRQLPNK VVKLDKLPVHVYEVDEEVDKDEDAASLGSQKGGITKHGWLYKGNMNSAISVTMRSFKRRFFHLIQLGDGS YNLNFYKDEKISKEPKGSIFLDSCMGVVQNNKVRRFAFELKMQDKSSYLLAADSEVEMEEWITILNKILQ LNFEAAMQEKRNGDSHEDDEQSKLEGSGSGLDSYLPELAKSAREAEIKLKSESRVKLFYLDPDAQKLDFS SAEPEVKSFEEKFGKRILVKCNDLSFNLQCCVAENEEGPTTNVEPFFVTLSLFDIKYNRKISADFHVDLN HFSVRQMLATTSPALMNGSGQSPSVLKGILHEAAMQYPKQGIFSVTCPHPDIFLVARIEKVLQGSITHCA EPYMKSSDSSKVAQKVLKNAKQACQRLGQYRMPFAWAARTLFKDASGNLDKNARFSAIYRQDSNKLSNDD MLKLLADFRKPEKMAKLPVILGNLDITIDNVSSDFPNYVNSSYIPTKQFETCSKTPITFEVEEFVPCIPK HTQPYTIYTNHLYVYPKYLKYDSQKSFAKARNIAICIEFKDSDEEDSQPLKCIYGRPGGPVFTRSAFAAV LHHHQNPEFYDEIKIELPTQLHEKHHLLLTFFHVSCDNSSKGSTKKRDVVETQVGYSWLPLLKDGRVVTS EQHIPVSANLPSGYLGYQELGMGRHYGPEIKWVDGGKPLLKISTHLVSTVYTQDQHLHNFFQYCQKTESG AQALGNELVKYLKSLHAMEGHVMIAFLPTILNQLFRVLTRATQEEVAVNVTRVIIHVVAQCHEEGLESHL RSYVKYAYKAEPYVASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFDVLIKSMAQHLIENSKV KLLRNQRFPASYHHAVETVVNMLMPHITQKFRDNPEASKNANHSLAVFIKRCFTFMDRGFVFKQINNYIS CFAPGDPKTLFEYKFEFLRVVCNHEHYIPLNLPMPFGKGRIQRYQDLQLDYSLTDEFCRNHFLVGLLLRE VGTALQEFREVRLIAISVLKNLLIKHSFDDRYASRSHQARIATLYLPLFGLLIENVQRINVRDVSPFPVN AGMTVKDESLALPAVNPLVTPQKGSTLDNSLHKDLLGAISGIASPYTTSTPNINSVRNADSRGSLISTDS GNSLPERNSEKSNSLDKHQQSSTLGNSVVRCDKLDQSEIKSLLMCFLYILKSMSDDALFTYWNKASTSEL MDFFTISEVCLHQFQYMGKRYIARTGMMHARLQQLGSLDNSLTFNHSYGHSDADVLHQSLLEANIATEVC LTALDTLSLFTLAFKNQLLADHGHNPLMKKVFDVYLCFLQKHQSETALKNVFTALRSLIYKFPSTFYEGR ADMCAALCYEILKCCNSKLSSIRTEASQLLYFLMRNNFDYTGKKSFVRTHLQVIISVSQLIADVVGIGGT RFQQSLSIINNCANSDRLIKHTSFSSDVKDLTKRIRTVLMATAQMKEHENDPEMLVDLQYSLAKSYASTP ELRKTWLDSMARIHVKNGDLSEAAMCYVHVTALVAEYLTRKEAVQWEPPLLPHSHSACLRRSRGGVFRQG CTAFRVITPNIDEEASMMEDVGMQDVHFNEDVLMELLEQCADGLWKAERYELIADIYKLIIPIYEKRRDF ERLAHLYDTLHRAYSKVTEVMHSGRRLLGTYFRVAFFGQAAQYQFTDSETDVEGFFEDEDGKEYIYKEPK LTPLSEISQRLLKLYSDKFGSENVKMIQDSGKVNPKDLDSKYAYIQVTHVIPFFDEKELQERKTEFERSH NIRRFMFEMPFTQTGKRQGGVEEQCKRRTILTAIHCFPYVKKRIPVMYQHHTDLNPIEVAIDEMSKKVAE LRQLCSSAEVDMIKLQLKLQGSVSVQVNAGPLAYARAFLDDTNTKRYPDNKVKLLKEVFRQFVEACGQAL AVNERLIKEDQLEYQEEMKANYREMAKELSEIMHEQLG SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001130048 |
ORF Size | 6204 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130048.2 |
RefSeq ORF | 6207 bp |
Locus ID | 23348 |
UniProt ID | Q9BZ29 |
MW | 236.2 kDa |
Gene Summary | Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.[UniProtKB/Swiss-Prot Function] |
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