GREB1L (NM_001142966) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C18orf6; KIAA1772; RHDA3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC226637 representing NM_001142966 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAATTCATATGCTGGGCAACTGAAATCTGCTCGATTTGAGGAAGCTCTCCACAACTCCATAGAAG CCTCCCTCAGATGTAGTAGTGTGGTACCACGGCCAATTTTTTCCCAGCTATACCTGGACCCTGACCAGCA TCCTTTCTCATCTGCAGATGTCAAACCCAAGGTGGAGGATCTGGACAAAGATTTGGTAAACCGCTACACT CAAAATGGAAGTCTGGATTTTTCTAACAATCTAACAGTTAATGAAATGGAAGATGATGAAGACGATGAAG AAATGTCTGATTCAAACAGCCCACCAATTCCCTATTCACAAAAACCTGCCCCAGAAGGATCTTGCACTAC AGATGGTTTTTGTCAAGCAGGAAAGGATTTGCGTTTGGTATCACTGTGTATGGAACAAATTGACATCCCA GCAGGATTCCTCCTGGTGGGGGCCAAGTCTCCCAATCTGCCTGAACACATCCTAGTTTGTGCTGTGGACA AGCGATTTCTACCAGATGATCATGGAAAAAATGCACTTTTAGGGTTTTCTGGAAATTGTATCGGCTGTGG AGAAAGAGGATTTCGATATTTCACGGAATTTTCCAACCACATTAACTTGAAGCTCACCACTCAGCCAAAG AAGCAGAAGCACTTAAAGTACTACCTAGTCAGAAGCTCCCAGGGTGTACTGTCTAAAGGACCTCTTATCT GCTGGAAAGAATGTAGAAGCCGACAATCCTCTGCTTCTTGCCACTCTATTAAGCCAAGCTCTTCAGTGTC GTCAACTGTGACCCCAGAAAATGGGACAACTAATGGATACAAATCAGGATTCACTCAGACAGATGCTGCT AATGGAAACAGTAGCCATGGAGGGAAGGGCAGTGCATCCAGCTCCACTCCAGCCCACACAGGGAATTACT CTTTGTCACCACGACCTAGCTATGCATCAGGAGATCAAGCTACCATGTTCATTTCTGGGCCACCAAAGAA ACGACACCGGGGATGGTATCCTGGGTCACCTCTCCCCCAACCTGGCTTAGTTGTACCTGTCCCTACAGTT CGCCCTCTTTCAAGAACGGAGCCCCTTTTATCTGCCCCAGTTCCACAGACCCCACTAACTGGAATTTTAC AACCCAGGCCCATTCCTGCAGGGGAAACTGTAATTGTTCCTGAAAACCTGCTGAGTAACTCAGGAGTTAG ACCAGTAATTCTGATAGGCTATGGCACTTTACCCTATTTCTATGGAAATGTTGGTGACATTGTTGTGAGT CCTCTGCTGGTGAATTGCTACAAGATTCCACAGTTGGAAAACAAAGATTTGGAAAAATTAGGGTTGACCG GCAGCCAATTTCTGAGCGTGGAAAACATGATTCTTCTGACGATACAGTATCTTGTGCGGCTGGGTCCTGA TCAGGTACCTCTCAGGGAAGAATTTGAGCAAATTATGCTGAAAGCTATGCAAGAATTTACTCTGAGAGAA AGAGCCCTGCAGATAGGTGCTCAGTGTGTCCCTGTGTCACCAGGACAACTCCCCTGGCTTGCTAGATTAA TTGCCAGCGTATCTCAAGACTTGGTTCATGTGGTTGTGACCCAGAACTCTTTGGCGGAGGGCATTTCAGA GACCTTAAGGACTCTCAGTGAAATGAGACACTATCAAAGGCTGCCAGATTATGTGGTGGTAATTTGTGCA TCGAAAATCAGAGGAAATGAATTTTGTGTGGTAGTGTTAGGTCAGCACCAGTCCCGAGCTCTGGCAGAGA GCATGCTCACCACAAGTGAGTTTTTGAAAGAAATTAGTTATGAGCTTATCACAGGAAAGGTCAGTTTCCT GGCATCACATTTCAAAACCACATCATTAGGCGATGACCTAGACAAGCTGCTGGAAAAAATGCAACAAAGA AGAGGAGACAGTGTGGTTACCCCTTTCGATGGAGACCTTAATGAATGTGTGTCACCCCAGGAGGCTGCTG CTATGATTCCCACACAAAACCTGGATTTAGATAATGAAACCTTCCACATTTATCAACCGCAGCTAACAGT TGCCAGAAAACTCTTATCCCAGGTGTGTGCTATAGCGGACAGTGGCAGCCAGAGCCTGGACCTCGGTCAC TTCAGCAAAGTAGACTTCATCATCATTGTTCCCAGATCGGAGGTGTTGGTTCAGCAAACTCTTCAGCGGA TTCGACAATCAGGTGTCCTTGTAGACTTGGGTCTGGAGGAAAATGGGACAGCCCATCAGAGAGCAGAAAA ATATGTTGTTCGTCTAGACAATGAGATTCAAACCAAGTTTGAAGTTTTTATGAGGAGAGTGAAACAGAAC CCGTACACACTGTTTGTGCTAGTTCATGACAACTCCCATGTGGAACTAACGAGTGTCATTTCAGGCTCTT TGTCACATAGCGAACCCAGTCATGGGCTAGCTGATAGAGTCATTAATTGCAGAGAAGTTCTGGAAGCTTT CAACCTCCTGGTGCTCCAGGTCAGCTCCTTCCCATACACTCTGCAGACCCAACAGTCCCGCATTAGCTCT AGCAATGAGGTTCACTGGATACAGCTGGATACTGGGGAGGACGTGGGCTGCGAGGAGAAGCTGTACTTTG GCTTGAGTGAGTACAGCAAGTCTCTGCAGTGGGGGATCACGAGCCCACTTCTGAGATGTGACGAGACTTT TGAAAAAATGGTGAACACACTCTTGGAGAGGTACCCCAGGCTGCACAGCATGGTCGTCCGCTGCTATCTT CTCATCCAGCAGTACTCTGAGGCCCTGATGGCTCTCACCACCATGGCGTCACTCCGCGACCACAGCACAC CAGAAACACTCAGCATTATGGATGACCTCATCAGCTCCCCAGGCAAAAACAAAAGTGGGAGGGGACACAT GCTCATTATCAGGGTGCCCTCGGTGCAGCTGGCGATGTTAGCCAAGGAGCGGCTACAGGAGGTGCGCGAC AAACTGGGTCTGCAGTATCGGTTTGAGATCATCCTTGGGAACCCGGCCACCGAACTCAGTGTTGCAACTC ACTTTGTGGCGCGATTAAAGAGTTGGAGAGGAAATGAACCAGAAGAGTGGATCCCTCGGACATACCAAGA TTTAGACGGTCTACCTTGTATTGTGATATTAACTGGCAAAGATCCTCTTGGAGAAACCTTTCCCAGGTCT TTGAAGTACTGTGACCTCCGGTTAATTGACTCAAGCTATTTAACTCGCACGGCCTTGGAGCAGGAGGTGG GTCTGGCATGCTGCTATGTCTCAAAAGAGGTCATCCGGGGACCCACTGTTGCCCTGGACCTCAGCGGGAA GGAGCAGGAGAGAGCTGCTGTCAGTGAGAATGACTCCGATGAGCTGCTCATCGACCTGGAGCGGCCCCAG AGCAACAGCAGCGCTGTCACAGGGACCTCGGGTTCCATCATGGAGAATGGAGTGAGCTCTTCCAGCACAG CTGACAAGTCCCAGAAGCAGTCCCTGACCCCCAGCTTTCAGAGCCCAGCCACCAGCTTGGGGCTGGATGA AGGGGTCTCCGCCAGCTCAGCTGGAGCCGGAGCCGGGGAGACTCTGAAGCAGGAATGTGACTCCCTGGGC CCCCAGATGGCGAGCAGCACCACCTCCAAGCCGTCATCATCATCCTCAGGACCCAGGACCCTCCCATGGC CGGGACAGCCCATCAGAGGCTGCCGGGGCCCACAGGCAGCCCTGCCACCAGTGGTGATCCTATCCAAAGC GGCCTACAGTCTCCTGGGCTCCCAGAAGAGTGGCAAGCTGCCATCCTCCTCCTCCCTGCTGCCCCACGCC GACGTGGCCTGGGTGAGCTCCCTGCGGCCACTCCTGAACAAGGACATGAGCAGTGAGGAGCAGTCCCTCT ACTACAGGCAGTGGACCTTGGCCCGGCAGCACCACGCTGACTATAGCAACCAGCTGGACCCGGCCTCTGG CACCCGAAACTTCCACCCCCGACGGCTCCTGCTGACAGGGCCCCCACAGGTGGGAAAGACGGGCTCCTAT TTACAGTTCCTCAGGATCCTCTTCCGCATGCTCATCAGGCTCCTGGAGGTGGACGTGTATGATGAGGAGG AGATCAACACCGATCACAATGAAAGCAGTGAAGTGAGCCAGTCAGAGGGAGAGCCCTGGCCTGACATCGA GAGCTTCAGTAAAATGCCTTTTGATGTCAGTGTGCATGACCCCAAGTACAGTTTGATGAGCCTGGTGTAT ACTGAGAAGCTGGCAGGGGTCAAACAAGAAGTGATAAAGGAATCCAAAGTTGAAGAGCCCAGGAAACGGG AAACTGTATCCATAATGCTGACCAAATATGCAGCCTATAACACCTTTCACCACTGTGAACAGTGCCGCCA GTACATGGACTTCACCTCTGCCTCCCAGATGTCTGACTCCACCCTTCATGCCTTCACATTCTCTTCTTCT ATGCTGGGAGAAGAGGTTCAGCTCTATTTCATCATTCCCAAATCCAAAGAGAGTCATTTTGTCTTCAGCA AGCAGGGCAAGCACCTGGAGAGCATGCGGCTGCCCCTGGTTTCAGACAAGAATTTGAATGCAGTCAAGAG CCCTATTTTCACTCCTTCCAGTGGTCGACATGAGCACGGACTCCTAAACCTTTTCCACGCCATGGAGGGC ATCAGCCACCTTCACCTCCTGGTGGTCAAAGAGTATGAGATGCCTCTGTACCGCAAGTACTGGCCCAACC ACATCATGCTGGTGCTTCCCGGCATGTTCAATAATGCAGGCGTGGGTGCTGCCAGGTTCCTCATCAAGGA GCTATCATATCACAACCTAGAATTGGAGAGAAACCGCCTGGAGGAGCTAGGCATTAAACGCCAGTGTGTC TGGCCTTTCATCGTCATGATGGATGACTCATGTGTCCTATGGAACATTCACAGTGTTCAGGAGCCATCCA GCCAGCCCATGGAAGTAGGAGTTTCCAGTAAGAATGTGTCCTTGAAGACTGTCTTGCAGCACATTGAAGC CACACCAAAAATTGTCCACTATGCAATCTTGGGCATACAGAAATGGAGCAGCAAGCTGACTTCTCAGAGC CTAAAGGCCCCATTCTCTAGGTGTCACGTGCATGATTTCATTCTCCTCAACACAGACTTGACTCAGAATG TGCAGTATGACTTCAACAGGTATTTCTGTGAAGATGCTGACTTTAATCTGAGAACAAACAGTAGTGGCCT GCTCATCTGCCGCTTTAATAACTTCAGTCTCATGAAGAAACATGTTCAGGTTGGAGGGCAAAGGGACTTT ATCATTAAACCAAAGATCATGGTGTCAGAGAGCTTAGCACCCATCTTGCCCCTTCAATACATCTGTGCTC CCGACAGTGAACACACACTACTGGCAGCCCCTGCACAGTTTCTCCTGGAGAAATTCCTTCAGCACGCCTC ATATAAACTCTTCCCCAAAGCCATCCATAACTTCAGGAGTCCTGTGCTGGCCATTGACTGCTACCTTAAC ATTGGACCAGAGGTGGCCATATGCTATATCAGCTCCAGACCCCACTCCAGCAATGTCAACTGTGAAGGGG TGTTTTTCAGTGGACTCCTTTTGTACCTCTGTGACTCTTTTGTAGGTGCTGATCTTAAGAAATTTAAATT TCTAAAAGGTGCTACACTGTGTGTCATCTGCCAAGACAGAAGTTCCTTGCGCCAAACAATTGTCCGCTTA GAGCTAGAAGATGAGTGGCAGTTCCGCCTCCGGGACGAGTTTCAAACTGCTAACAGCAGTGATGACAAGC CTCTCTACTTTCTTACTGGACGTCATGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC226637 representing NM_001142966 Red=Cloning site Green=Tags(s) MGNSYAGQLKSARFEEALHNSIEASLRCSSVVPRPIFSQLYLDPDQHPFSSADVKPKVEDLDKDLVNRYT QNGSLDFSNNLTVNEMEDDEDDEEMSDSNSPPIPYSQKPAPEGSCTTDGFCQAGKDLRLVSLCMEQIDIP AGFLLVGAKSPNLPEHILVCAVDKRFLPDDHGKNALLGFSGNCIGCGERGFRYFTEFSNHINLKLTTQPK KQKHLKYYLVRSSQGVLSKGPLICWKECRSRQSSASCHSIKPSSSVSSTVTPENGTTNGYKSGFTQTDAA NGNSSHGGKGSASSSTPAHTGNYSLSPRPSYASGDQATMFISGPPKKRHRGWYPGSPLPQPGLVVPVPTV RPLSRTEPLLSAPVPQTPLTGILQPRPIPAGETVIVPENLLSNSGVRPVILIGYGTLPYFYGNVGDIVVS PLLVNCYKIPQLENKDLEKLGLTGSQFLSVENMILLTIQYLVRLGPDQVPLREEFEQIMLKAMQEFTLRE RALQIGAQCVPVSPGQLPWLARLIASVSQDLVHVVVTQNSLAEGISETLRTLSEMRHYQRLPDYVVVICA SKIRGNEFCVVVLGQHQSRALAESMLTTSEFLKEISYELITGKVSFLASHFKTTSLGDDLDKLLEKMQQR RGDSVVTPFDGDLNECVSPQEAAAMIPTQNLDLDNETFHIYQPQLTVARKLLSQVCAIADSGSQSLDLGH FSKVDFIIIVPRSEVLVQQTLQRIRQSGVLVDLGLEENGTAHQRAEKYVVRLDNEIQTKFEVFMRRVKQN PYTLFVLVHDNSHVELTSVISGSLSHSEPSHGLADRVINCREVLEAFNLLVLQVSSFPYTLQTQQSRISS SNEVHWIQLDTGEDVGCEEKLYFGLSEYSKSLQWGITSPLLRCDETFEKMVNTLLERYPRLHSMVVRCYL LIQQYSEALMALTTMASLRDHSTPETLSIMDDLISSPGKNKSGRGHMLIIRVPSVQLAMLAKERLQEVRD KLGLQYRFEIILGNPATELSVATHFVARLKSWRGNEPEEWIPRTYQDLDGLPCIVILTGKDPLGETFPRS LKYCDLRLIDSSYLTRTALEQEVGLACCYVSKEVIRGPTVALDLSGKEQERAAVSENDSDELLIDLERPQ SNSSAVTGTSGSIMENGVSSSSTADKSQKQSLTPSFQSPATSLGLDEGVSASSAGAGAGETLKQECDSLG PQMASSTTSKPSSSSSGPRTLPWPGQPIRGCRGPQAALPPVVILSKAAYSLLGSQKSGKLPSSSSLLPHA DVAWVSSLRPLLNKDMSSEEQSLYYRQWTLARQHHADYSNQLDPASGTRNFHPRRLLLTGPPQVGKTGSY LQFLRILFRMLIRLLEVDVYDEEEINTDHNESSEVSQSEGEPWPDIESFSKMPFDVSVHDPKYSLMSLVY TEKLAGVKQEVIKESKVEEPRKRETVSIMLTKYAAYNTFHHCEQCRQYMDFTSASQMSDSTLHAFTFSSS MLGEEVQLYFIIPKSKESHFVFSKQGKHLESMRLPLVSDKNLNAVKSPIFTPSSGRHEHGLLNLFHAMEG ISHLHLLVVKEYEMPLYRKYWPNHIMLVLPGMFNNAGVGAARFLIKELSYHNLELERNRLEELGIKRQCV WPFIVMMDDSCVLWNIHSVQEPSSQPMEVGVSSKNVSLKTVLQHIEATPKIVHYAILGIQKWSSKLTSQS LKAPFSRCHVHDFILLNTDLTQNVQYDFNRYFCEDADFNLRTNSSGLLICRFNNFSLMKKHVQVGGQRDF IIKPKIMVSESLAPILPLQYICAPDSEHTLLAAPAQFLLEKFLQHASYKLFPKAIHNFRSPVLAIDCYLN IGPEVAICYISSRPHSSNVNCEGVFFSGLLLYLCDSFVGADLKKFKFLKGATLCVICQDRSSLRQTIVRL ELEDEWQFRLRDEFQTANSSDDKPLYFLTGRHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001142966
ORF Size	5769 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001142966.2
RefSeq ORF	5772 bp
Locus ID	80000
UniProt ID	Q9C091
MW	214.2 kDa
Gene Summary	Plays a major role in early metanephros and genital development.[UniProtKB/Swiss-Prot Function]

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