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CROT (NM_001143935) Human Tagged ORF Clone
CAT＃: RC226801
CROT (Myc-DDK-tagged)-Human carnitine O-octanoyltransferase (CROT), transcript variant 1
ORF Plasmid: tGFP
"NM_001143935" in other vectors (4)
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CNY 4,768.00
CNY 7,505.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | COT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226801 representing NM_001143935
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAATCAATTGGCTAAATCAACTGAAGAACGAACATTTCAGTACCAGGATTCTCTTCCATCACTGC CTGTTCCTTCACTTGAAGAATCATTAAAAAAATACCTTGAATCAGTCACCAGAACATGCTACCAGATAAG GGGTCTTGATCCAGATGCTAAGAGAGGGTTCTTGGATCTCACGCGGGAAGGAATTCAAGTGAAACCATTT GCAAATCAAGAAGAATATAAGAAAACTGAAGAAATAGTTCAAAAATTTCAAAGTGGGATTGGAGAAAAAT TGCACCAGAAATTGCTTGAAAGAGCAAAAGGAAAAAGAAATTGGCTGGAAGAGTGGTGGCTGAATGTTGC CTATCTGGATGTTCGTATACCATCACAATTGAATGTCAACTTTGCGGGTCCTGCAGCTCATTTTGAACAC TACTGGCCTCCAAAGGAAGGGACTCAATTAGAAAGAGGAAGTATAACTCTTTGGCATAACTTGAACTACT GGCAGCTATTAAGAAAAGAAAAAGTGCCTGTTCATAAAGTTGGAAATACTCCTCTAGATATGAATCAATT CCGAATGCTATTTTCTACCTGCAAGGTTCCAGGAATTACTAGAGACTCCATTATGAATTATTTTAGGACT GAGAGTGAAGGGCGTTCCCCAAACCACATTGTAGTGCTGTGTCGAGGCCGAGCTTTTGTCTTTGATGTAA TACATGAAGGATGTTTGGTCACCCCGCCAGAGCTTCTCAGACAACTGACATATATCCACAAGAAGTGCCA TAGTGAACCTGATGGACCTGGGATTGCAGCATTAACTAGTGAGGAGCGAACTCGATGGGCTAAGGCACGA GAATATCTGATTGGTCTTGATCCAGAGAACTTGGCTTTGTTAGAAAAAATTCAGAGTAGTTTACTGGTAT ATTCCATGGAGGATAGCAGTCCACATGTAACACCAGAGGATTATTCTGAGATTATTGCAGCCATCCTTAT TGGAGATCCAACAGTACGCTGGGGTGACAAATCCTATAACTTGATTTCCTTTTCTAATGGAGTATTTGGC TGTAATTGTGATCATGCTCCTTTTGATGCAATGATTATGGTGAACATCAGTTATTATGTGGATGAGAAAA TTTTTCAGAATGAAGGAAGATGGAAGGGTTCAGAGAAGGTACGAGATATACCACTTCCAGAAGAGCTCAT TTTCATTGTGGATGAGAAAGTTTTAAATGACATCAACCAAGCTAAAGCCCAGTATCTCAGGGAGGCATCT GATCTACAGATTGCGGCTTATGCCTTTACATCTTTTGGCAAAAAGCTAACCAAGAACAAGATGCTTCACC CGGATACGTTTATTCAGCTTGCACTTCAGCTGGCCTATTACAGACTTCATGGACACCCTGGTTGTTGCTA TGAAACAGCTATGACAAGACATTTTTATCATGGCCGTACAGAGACTATGCGATCATGCACAGTTGAAGCA GTGAGGTGGTGCCAGTCCATGCAGGATCCTTCTGTCAATCTTCGTGAGCGGCAGCAAAAGATGTTACAAG CTTTTGCAAAGCATAATAAAATGATGAAAGATTGTTCAGCTGGAAAAGGATTTGATCGTCACCTTTTAGG TCTCTTACTCATAGCAAAAGAGGAAGGTCTTCCTGTTCCAGAACTCTTTACGGACCCACTTTTTTCCAAA AGCGGAGGAGGTGGAAATTTTGTTCTCTCAACAAGTCTGGTTGGCTATTTACGAGTCCAGGGAGTGGTAG TTCCCATGGTACACAATGGTTATGGATTTTTCTACCATATCAGAGATGACAGGTTTGTTGTGGCCTGTTC AGCCTGGAAATCCTGTCCCGAGACTGATGCGGAAAAGCTAGTTCAGCTGACTTTTTGTGCTTTTCATGAT ATGATACAGCTGATGAACTCTACTCATCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226801 representing NM_001143935
Red=Cloning site Green=Tags(s) MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVTRTCYQIRGLDPDAKRGFLDLTREGIQVKPF ANQEEYKKTEEIVQKFQSGIGEKLHQKLLERAKGKRNWLEEWWLNVAYLDVRIPSQLNVNFAGPAAHFEH YWPPKEGTQLERGSITLWHNLNYWQLLRKEKVPVHKVGNTPLDMNQFRMLFSTCKVPGITRDSIMNYFRT ESEGRSPNHIVVLCRGRAFVFDVIHEGCLVTPPELLRQLTYIHKKCHSEPDGPGIAALTSEERTRWAKAR EYLIGLDPENLALLEKIQSSLLVYSMEDSSPHVTPEDYSEIIAAILIGDPTVRWGDKSYNLISFSNGVFG CNCDHAPFDAMIMVNISYYVDEKIFQNEGRWKGSEKVRDIPLPEELIFIVDEKVLNDINQAKAQYLREAS DLQIAAYAFTSFGKKLTKNKMLHPDTFIQLALQLAYYRLHGHPGCCYETAMTRHFYHGRTETMRSCTVEA VRWCQSMQDPSVNLRERQQKMLQAFAKHNKMMKDCSAGKGFDRHLLGLLLIAKEEGLPVPELFTDPLFSK SGGGGNFVLSTSLVGYLRVQGVVVPMVHNGYGFFYHIRDDRFVVACSAWKSCPETDAEKLVQLTFCAFHD MIQLMNSTHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001143935 |
| ORF Size | 1920 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001143935.2 |
| RefSeq ORF | 1923 bp |
| Locus ID | 54677 |
| UniProt ID | Q9UKG9 |
| Protein Families | Druggable Genome |
| MW | 73.2 kDa |
| Gene Summary | This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226801L3 | Lenti-ORF clone of CROT (Myc-DDK-tagged)-Human carnitine O-octanoyltransferase (CROT), transcript variant 1 |
CNY 5,890.00 |
|
| RC226801L4 | Lenti-ORF clone of CROT (mGFP-tagged)-Human carnitine O-octanoyltransferase (CROT), transcript variant 1 |
CNY 5,890.00 |
|
| RG226801 | CROT (tGFP-tagged) - Human carnitine O-octanoyltransferase (CROT), transcript variant 1 |
CNY 4,370.00 |
|
| SC326138 | CROT (untagged)-Human carnitine O-octanoyltransferase (CROT), transcript variant 1 |
CNY 10,260.00 |
