DOHH (NM_001145165) Human Tagged ORF Clone
CAT#: RC226938
DOHH (Myc-DDK-tagged)-Human deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant 1
ORF Plasmid: tGFP
"NM_001145165" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | hDOHH; HLRC1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC226938 representing NM_001145165
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGACGGAGCAGGAGGTGGATGCCATCGGGCAGACGCTGGTGGACCCCAAGCAGCCCCTGCAGGCCC GCTTCCGGGCGCTGTTCACGCTGCGTGGGCTCGGCGGCCCAGGCGCCATTGCATGGATCAGCCAGGCCTT CGATGACGATTCCGCCCTGCTCAAGCACGAGCTGGCCTACTGCCTGGGCCAGATGCAGGATGCCCGCGCC ATCCCCATGCTGGTGGACGTGCTGCAAGACACCCGTCAGGAGCCCATGGTGCGCCATGAGGCAGGGGAGG CCCTGGGGGCCATCGGGGACCCGGAAGTTCTGGAGATCCTGAAGCAGTATTCCTCGGACCCCGTCATCGA GGTGGCCGAGACCTGCCAGCTGGCCGTGCGCAGGCTGGAGTGGCTGCAGCAGCACGGCGGGGAGCCGGCG GCGGGACCCTACCTCTCCGTGGACCCTGCCCCGCCGGCTGAGGAGCGTGACGTGGGGCGCCTGCGGGAGG CGCTGCTGGATGAGTCCCGGCCGCTCTTCGAGCGATACCGCGCCATGTTCGCCCTGCGCAACGCGGGAGG CGAGGAGGCCGCCCTGGCGCTGGCCGAGGGTCTGCACTGTGGGAGCGCCCTCTTCCGCCACGAGGTCGGC TACGTCCTGGGACAGCTGCAGCACGAGGCGGCGGTGCCCCAGCTGGCGGCCGCCCTGGCCCGATGCACCG AGAACCCCATGGTGCGGCACGAGTGCGCGGAGGCCCTGGGCGCCATTGCCCGGCCCGCCTGCCTGGCCGC GCTGCAGGCTCACGCGGACGACCCAGAGCGCGTGGTGCGTGAGAGCTGCGAGGTGGCTCTGGACATGTAT GAGCACGAGACCGGGCGGGCCTTCCAGTACGCGGACGGCCTGGAGCAGCTGCGCGGGGCCCCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226938 representing NM_001145165
Red=Cloning site Green=Tags(s) MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARA IPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPA AGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVG YVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMY EHETGRAFQYADGLEQLRGAPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145165 |
ORF Size | 906 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145165.2 |
RefSeq ORF | 909 bp |
Locus ID | 83475 |
UniProt ID | Q9BU89 |
MW | 32.7 kDa |
Gene Summary | This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226938L1 | Lenti ORF clone of Human deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC226938L2 | Lenti ORF clone of Human deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC226938L3 | Lenti ORF clone of Human deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC226938L4 | Lenti ORF clone of Human deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG226938 | DOHH (tGFP-tagged) - Human deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant 1 |
CNY 4,370.00 |
|
SC324917 | DOHH (untagged)-Human deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant 1 |
CNY 6,270.00 |