FAM178A (SLF2) (NM_001136123) Human Tagged ORF Clone
CAT#: RC227146
- TrueORF®
FAM178A (Myc-DDK-tagged)-Human family with sequence similarity 178, member A (FAM178A), transcript variant 2
ORF Plasmid: tGFP
"NM_001136123" in other vectors (6)
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CNY 8,256.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C10orf6; FAM178A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227146 representing NM_001136123
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAGGCGCTGCATGCCCGCTAGGCCAGGTTTCCCCTCATCCCCAGCCCCGGGGTCGTCGCCCCCGC GCTGCCATCTGAGACCCGGTAGTACCGCCCATGCTGCAGCGGGAAAGAGAACAGAGAGTCCTGGGGACAG GAAGCAGTCAATTATAGATTTCTTCAAACCAGCTTCAAAACAAGACAGACACATGTTGGATTCACCACAA AAATCAAACATCAAATATGGAGGAAGTAGATTGTCTATCACTGGGACAGAGCAGTTTGAAAGGAAACTAT CCTCACCAAAAGAATCTAAACCCAAAAGGGTGCCACCAGAAAAGAGCCCTATTATAGAAGCTTTCATGAA AGGTGTTAAAGAGCACCATGAAGATCATGGTATACATGAGTCACGTCGGCCTTGTCTGTCACTAGCCTCC AAATATTTAGCCAAAGGAACAAATATCTATGTTCCTTCTTCATATCACTTGCCAAAGGAGATGAAGTCAC TAAAGAAAAAACATCGATCCCCAGAGAGAAGGAAGTCACTATTCATTCATGAAAATAATGAGAAGAATGA TAGAGATCGAGGCAAAACCAATGCAGACTCCAAAAAGCAGACCACAGTGGCAGAAGCTGACATCTTCAAT AACAGCTCCAGAAGCCTTAGCAGCAGGAGCAGCCTGTCCAGGCACCACCCGGAAGAAAGCCCACTGGGAG CTAAATTCCAGTTGTCACTAGCTTCTTACTGCAGAGAACGAGAACTAAAGAGGTTGAGAAAGGAGCAAAT GGAGCAGAGAATCAACTCCGAGAATTCTTTCTCAGAAGCAAGCAGTCTTTCCTTAAAATCTAGTATAGAA AGAAAATATAAACCAAGGCAGGAACAAAGGAAACAGAATGACATCATACCTGGAAAAAATAATCTGTCAA ATGTGGAAAATGGACATCTCTCAAGAAAAAGATCCTCTTCTGATTCATGGGAACCTACTTCAGCAGGCTC TAAGCAGAATAAATTCCCTGAAAAAAGAAAAAGGAACTCTGTGGACTCAGATCTGAAAAGCACAAGAGAA TCTATGATACCAAAAGCAAGAGAGTCCTTCCTTGAGAAGCGTCCTGATGGACCACATCAGAAAGAAAAAT TTATAAAACATATTGCACTGAAGACACCTGGTGATGTGTTGCGCTTAGAAGATATATCCAAGGAACCGAG TGATGAAACTGATGGCTCTTCTGCAGGCTTGGCACCTTCAAATTCTGGCAATTCTGGCCACCATTCTACC AGGAATAGTGACCAAATCCAAGTGGCAGGTACCAAGGAGACTAAGATGCAGAAACCCCACTTACCTTTAT CTCAGGAAAAGTCTGCAATTAAAAAAGCTAGCAACCTTCAGAAAAATAAAACCGCTAGCTCCACGACAAA GGAGAAGGAGACAAAACTACCTTTACTTTCCCGTGTTCCAAGTGCTGGTTCCTCTCTAGTACCATTAAAT GCTAAAAATTGTGCTCTTCCAGTTTCTAAAAAAGATAAAGAGCGTTCCTCATCTAAAGAATGTTCTGGGC ATTCTACAGAATCCACCAAACACAAGGAACACAAAGCAAAGACTAATAAGGCCGATTCTAATGTATCTTC AGGGAAAATTTCTGGGGGACCTTTGCGCTCAGAATATGGCACTCCTACAAAGTCTCCCCCTGCTGCTTTG GAAGTTGTGCCATGTATCCCAAGCCCTGCAGCACCTTCAGATAAAGCCCCTTCAGAAGGAGAGAGTTCAG GAAATTCCAATGCAGGTAGCAGTGCACTGAAAAGAAAACTAAGGGGTGATTTTGATAGTGATGAAGAAAG TTTAGGTTACAACCTAGACAGTGATGAGGAAGAGGAAACATTAAAGTCACTGGAAGAAATAATGGCTTTG AACTTCAATCAGACTCCTGCAGCTACAGGAAAGCCTCCTGCTCTTTCCAAGGGGCTTAGATCTCAGTCAT CAGACTATACAGGACATGTTCATCCTGGAACTTACACAAATACCTTAGAACGTCTAGTGAAGGAAATGGA AGACACACAAAGGCTAGATGAACTGCAGAAGCAACTACAAGAAGACATAAGGCAAGGCCGAGGCATTAAA TCCCCAATCAGAATTGGAGAAGAAGACAGTACAGATGATGAGGATGGCCTCTTAGAAGAGCACAAGGAAT TTCTAAAGAAATTTTCAGTTACAATTGATGCTATTCCTGATCATCATCCAGGTGAAGAAATATTTAATTT CCTCAATTCTGGAAAAATTTTCAATCAGTATACCTTGGATTTAAGAGACTCTGGTTTTATTGGACAAAGT GCTGTAGAAAAACTTATTCTTAAATCGGGAAAAACAGATCAGATTTTTTTGACAACACAAGGTTTCCTTA CGTCTGCTTATCACTATGTCCAGTGTCCTGTCCCTGTGTTAAAGTGGCTGTTTCGGATGATGTCAGTTCA TACAGACTGTATTGTGTCAGTGCAGATTTTAAGTACATTGATGGAAATAACAATTAGAAATGATACCTTC AGTGACTCACCAGTTTGGCCATGGATCCCATCATTGTCTGATGTAGCAGCTGTGTTTTTCAATATGGGGA TTGATTTTAGATCTTTGTTTCCCCTGGAGAATCTTCAGCCAGACTTTAATGAAGACTATCTAGTTTCTGA AACACAGACAACATCAAGGGGGAAAGAAAGTGAAGATTCATCTTATAAGCCAATTTTTTCAACACTTCCT GAAACCAACATTTTAAATGTGGTTAAGTTTCTAGGCTTGTGTACATCTATACATCCAGAAGGTTACCAGG ATCGTGAAATAATGTTGCTGATTTTAATGTTATTTAAAATGAGTTTGGAAAAACAGCTGAAACAGATTCC TTTAGTAGACTTTCAAAGCCTCCTGATAAACCTGATGAAAAACATCAGAGATTGGAACACAAAGGTGCCT GAACTCTGTCTGGGCATAAATGAACTCTCCAGTCATCCCCACAACCTCCTGTGGTTGGTACAGCTGGTCC CTAATTGGACATCACGTGGAAGGCAACTGAGACAGTGCCTCAGTCTAGTGATTATTTCAAAGCTTTTGGA TGAGAAACACGAAGATGTTCCTAATGCCAGTAATCTGCAGGTATCAGTCCTACATCGCTATCTTGTGCAG ATGAAGCCTTCTGATTTGTTAAAGAAAATGGTCTTGAAGAAAAAGGCTGAACAACCAGATGGCATTATTG ATGACAGTCTTCATTTAGAACTTGAAAAGCAGGCATATTACCTGACCTACATTCTTCTTCATTTAGTCGG TGAAGTTAGTTGTTCTCATTCTTTTTCTTCTGGACAACGGAAACACTTTGTGCTACTCTGTGGGGCTTTG GAAAAGCATGTTAAATGTGATATTAGGGAAGATGCAAGACTTTTTTACAGAACTAAGGTGAAAGACTTGG TCGCCAGGATACATGGAAAATGGCAGGAAATAATCCAGAACTGTCGGCCTACTCAGGTGTCATTTTGTTA TACAATTTCATGTATTCTTAATAGTTTTGCTGAATGGCACTCTTCATACTGTTTGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227146 representing NM_001136123
Red=Cloning site Green=Tags(s) MTRRCMPARPGFPSSPAPGSSPPRCHLRPGSTAHAAAGKRTESPGDRKQSIIDFFKPASKQDRHMLDSPQ KSNIKYGGSRLSITGTEQFERKLSSPKESKPKRVPPEKSPIIEAFMKGVKEHHEDHGIHESRRPCLSLAS KYLAKGTNIYVPSSYHLPKEMKSLKKKHRSPERRKSLFIHENNEKNDRDRGKTNADSKKQTTVAEADIFN NSSRSLSSRSSLSRHHPEESPLGAKFQLSLASYCRERELKRLRKEQMEQRINSENSFSEASSLSLKSSIE RKYKPRQEQRKQNDIIPGKNNLSNVENGHLSRKRSSSDSWEPTSAGSKQNKFPEKRKRNSVDSDLKSTRE SMIPKARESFLEKRPDGPHQKEKFIKHIALKTPGDVLRLEDISKEPSDETDGSSAGLAPSNSGNSGHHST RNSDQIQVAGTKETKMQKPHLPLSQEKSAIKKASNLQKNKTASSTTKEKETKLPLLSRVPSAGSSLVPLN AKNCALPVSKKDKERSSSKECSGHSTESTKHKEHKAKTNKADSNVSSGKISGGPLRSEYGTPTKSPPAAL EVVPCIPSPAAPSDKAPSEGESSGNSNAGSSALKRKLRGDFDSDEESLGYNLDSDEEEETLKSLEEIMAL NFNQTPAATGKPPALSKGLRSQSSDYTGHVHPGTYTNTLERLVKEMEDTQRLDELQKQLQEDIRQGRGIK SPIRIGEEDSTDDEDGLLEEHKEFLKKFSVTIDAIPDHHPGEEIFNFLNSGKIFNQYTLDLRDSGFIGQS AVEKLILKSGKTDQIFLTTQGFLTSAYHYVQCPVPVLKWLFRMMSVHTDCIVSVQILSTLMEITIRNDTF SDSPVWPWIPSLSDVAAVFFNMGIDFRSLFPLENLQPDFNEDYLVSETQTTSRGKESEDSSYKPIFSTLP ETNILNVVKFLGLCTSIHPEGYQDREIMLLILMLFKMSLEKQLKQIPLVDFQSLLINLMKNIRDWNTKVP ELCLGINELSSHPHNLLWLVQLVPNWTSRGRQLRQCLSLVIISKLLDEKHEDVPNASNLQVSVLHRYLVQ MKPSDLLKKMVLKKKAEQPDGIIDDSLHLELEKQAYYLTYILLHLVGEVSCSHSFSSGQRKHFVLLCGAL EKHVKCDIREDARLFYRTKVKDLVARIHGKWQEIIQNCRPTQVSFCYTISCILNSFAEWHSSYCLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001136123 |
ORF Size | 3558 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001136123.2 |
RefSeq ORF | 3561 bp |
Locus ID | 55719 |
UniProt ID | Q8IX21 |
MW | 133.2 kDa |
Gene Summary | Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions (PubMed:25931565).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components
,Alabert, C;Bukowski-Wills, JC;Lee, SB;Kustatscher, G;Nakamura, K;de Lima Alves, F;Menard, P;Mejlvang, J;Rappsilber, J;Groth, A;,
Nat. Cell Biol., Feb 2014.
,PubMed ID 24561620
[SLF2]
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227146L1 | Lenti ORF clone of Human family with sequence similarity 178, member A (FAM178A), transcript variant 2, Myc-DDK-tagged |
CNY 14,440.00 |
|
RC227146L2 | Lenti ORF clone of Human family with sequence similarity 178, member A (FAM178A), transcript variant 2, mGFP tagged |
CNY 14,440.00 |
|
RC227146L3 | Lenti ORF clone of Human family with sequence similarity 178, member A (FAM178A), transcript variant 2, Myc-DDK-tagged |
CNY 14,440.00 |
|
RC227146L4 | Lenti ORF clone of Human family with sequence similarity 178, member A (FAM178A), transcript variant 2, mGFP tagged |
CNY 14,440.00 |
|
RG227146 | FAM178A (tGFP-tagged) - Human family with sequence similarity 178, member A (FAM178A), transcript variant 2 |
CNY 13,780.00 |
|
SC326351 | FAM178A (untagged)-Human family with sequence similarity 178, member A (FAM178A), transcript variant 2 |
CNY 22,800.00 |
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