PTGES3L-AARSD1 (NM_001136042) Human Tagged ORF Clone

CAT#: RC227250

PTGES3L (Myc-DDK-tagged)-Human alanyl-tRNA synthetase domain containing 1 (AARSD1), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_001136042" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 4,360.00

CNY 5,130.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Mouse monoclonal Tsp23 Antibody
    • 100 ug

CNY 5,511.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC227250 representing NM_001136042
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTCTCTTCCTCTCAACTGCTCTCCCGACCATATAAGGAGAGGCAGCTGCTGGGGGCGTCCCCAGG
ATCTAAAGATAGCGGCCCCTGCCTGGAACTCTAAATGCCACCCTGGAGCGGGAGCCGCAATGGCACGGCA
GCACGCCCGGACCTTGTGGTACGACAGGCCCAGGTATGTGTTCATGGAGTTTTGTGTTGAGGACAGCACC
GATGTCCACGTGCTTATTGAGGATCACCGCATTGTGTTCAGCTGCAAGAATGCCGATGGAGTGGAGTTGT
ACAATGAGATTGAGTTCTATGCCAAAGTGAACTCCAAGGACTCCCAGGATAAGCGCTCTTCCCGCTCTAT
TACTTGTTTTGTGAGAAAATGGAAGGAAAAGGTGGCCTGGCCGCGGCTTACCAAGGAGGATATCAAGCCA
GTGTGGCTGTCTGTGGACTTTGATAACTGGAGAGACTGGGAAGGGGATGAAGAGATGGAGCTGGCTCATG
TGGAACATTATGCAGAGCTTTTGAAGAAGGTCAGCACCAAGAGACCTCCACCTGCCATGGATGATTTGGA
TTTCACCACCACCGTGGTCTCCTGCTGTCCCGCGGAGCTGCAGACTGAAGGGAGCAACGGCAAGAAAGAA
GTGCTGAGCGGTTTCCAAGTGGTGCTGGAAGACACAGTGCTTTTCCCTGAGGGCGGGGGACAGCCTGATG
ACCGTGGTACAATCAATGACATCTCTGTGCTGAGAGTGACTCGCCGTGGGGAACAGGCTGATCATTTCAC
CCAGACACCCCTGGATCCAGGAAGCCAGGTTCTGGTCCGGGTAGATTGGGAGCGGAGGTTTGACCACATG
CAGCAGCATTCAGGGCAGCATCTCATCACGGCAGTTGCTGACCATCTATTTAAGCTGAAGACAACATCAT
GGGAGTTAGGGAGATTTCGGAGTGCGATTGAGCTGGACACCCCCTCTATGACTGCAGAGCAAGTAGCTGC
CATTGAGCAGAGCGTCAATGAAAAAATCAGAGATCGGCTGCCTGTGAATGTCCGAGAACTGAGCCTGGAT
GATCCTGAGGTGGAGCAGGTGAGTGGCCGGGGTTTGCCTGATGATCATGCTGGGCCCATTCGGGTTGTTA
ACATCGAGGGCGTTGATTCCAACATGTGCTGTGGGACCCATGTGAGCAATCTCAGTGACCTTCAGGTCAT
TAAGATTCTGGGCACTGAGAAGGGGAAAAAGAACAGAACCAACCTGATATTTCTGTCTGGGAACCGGGTG
CTGAAGTGGATGGAGAGAAGTCATGGAACTGAAAAAGCACTGACTGCTCTGCTTAAGTGTGGAGCAGAGG
ATCATGTGGAAGCAGTGAAAAAGCTCCAGAACTCCACCAAGATCCTGCAGAAGAATAACCTGAATCTGCT
CAGAGACCTGGCTGTGCACATTGCCCATAGCCTCAGGAACAGTCCAGACTGGGGAGGTGTGGTCATATTA
CACAGGAAGGAGGGTGATTCAGAGTTCATGAATATCATTGCCAATGAGATTGGGTCAGAGGAGACCCTCC
TGTTCTTAACTGTGGGCGATGAGAAAGGTGGTGGACTCTTCTTACTGGCAGGGCCACCTGCGTCTGTGGA
GACCCTGGGGCCCAGGGTGGCTGAGGTCCTGGAAGGCAAAGGAGCAGGGAAGAAAGGCCGTTTTCAGGGC
AAGGCCACCAAGATGAGCCGGCGGATGGAGGCGCAGGCGCTTCTCCAGGACTACATCAGCACGCAGAGTG
CTAAGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC227250 representing NM_001136042
Red=Cloning site Green=Tags(s)

MFSLPLNCSPDHIRRGSCWGRPQDLKIAAPAWNSKCHPGAGAAMARQHARTLWYDRPRYVFMEFCVEDST
DVHVLIEDHRIVFSCKNADGVELYNEIEFYAKVNSKDSQDKRSSRSITCFVRKWKEKVAWPRLTKEDIKP
VWLSVDFDNWRDWEGDEEMELAHVEHYAELLKKVSTKRPPPAMDDLDFTTTVVSCCPAELQTEGSNGKKE
VLSGFQVVLEDTVLFPEGGGQPDDRGTINDISVLRVTRRGEQADHFTQTPLDPGSQVLVRVDWERRFDHM
QQHSGQHLITAVADHLFKLKTTSWELGRFRSAIELDTPSMTAEQVAAIEQSVNEKIRDRLPVNVRELSLD
DPEVEQVSGRGLPDDHAGPIRVVNIEGVDSNMCCGTHVSNLSDLQVIKILGTEKGKKNRTNLIFLSGNRV
LKWMERSHGTEKALTALLKCGAEDHVEAVKKLQNSTKILQKNNLNLLRDLAVHIAHSLRNSPDWGGVVIL
HRKEGDSEFMNIIANEIGSEETLLFLTVGDEKGGGLFLLAGPPASVETLGPRVAEVLEGKGAGKKGRFQG
KATKMSRRMEAQALLQDYISTQSAKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001136042
ORF Size 1758 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001136042.2, NP_001129514.2
RefSeq ORF 1761 bp
Locus ID 100885850
UniProt ID Q9BTE6
MW 65.5 kDa
Gene Summary This locus represents naturally occurring readthrough transcription between the neighboring PTGES3L (prostaglandin E synthase 3 (cytosolic)-like) and AARSD1(alanyl-tRNA synthetase domain containing 1) genes on chromosome 17. The readthrough transcript encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, May 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...