ENDOGL1 (EXOG) (NM_001145464) Human Tagged ORF Clone

CAT#: RC227897

EXOG (Myc-DDK-tagged)-Human endo/exonuclease (5'-3'), endonuclease G-like (EXOG), nuclear gene encoding mitochondrial protein, transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001145464" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


EXOG rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ENDOGL1; ENDOGL2; ENGL; ENGL-a; ENGL-b; ENGLA; ENGLB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC227897 representing NM_001145464
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTATCAAGAGTATCGCTTCCCGCCTCCGGGGTTCCCGTCGTTTTCTGAGCGGCTTCGTGGCTGGGG
CTGTAGTGGGCGCTGCGGGAGCTGGGCTCGCGGCCCTGCAGTTCTTCCGGAGTCAGGGCGCTGAGGGAGC
GTTGACAGGGAAGCAGCCGGATGGTGATGCAGACAGAAAGCATTGTAAATTTAAGCCTGATCCCAATATC
CCTCCAACCTTCAGTGCCTTCAATGAAGATTATGTTGGAAGTGGGTGGTCACGAGGACACATGGCTCCAG
CAGGAAATAACAAATTTTCAAGTAAAGCCATGGCTGAAACCTTTTACCTTTCTAACATTGTGCCTCAGGA
TTTTGATAATAATTCTGGATATTGGAACAGAATAGAAATGTACTGTCGAGAGCTGACAGAAAGGTTTGAA
GATGTTTGGGTGGTATCTGGGCCTTTGACCTTACCTCAGACTAGAGGCGATGGAAAGAAAATAGTTAGTT
ACCAGGTGATTGGCGAGGACAACGTGGCAGTCCCCTCACACCTTTATAAGGTAATCCTGGCCCGCAGAAG
CTCAGTATCTACCGAACCACTGGCGCTAGGGGCCTTTGTGGTACCCAATGAAGCCATCGGCTTCCAGCCC
CAGTTAACTGAATTCCAAGTGAGCCTCCAGGACCTAGAGAAGTTGTCAGGACTGGTGTTTTTTCCTCATT
TGGATAGAACTAGTGATATCCGGAATATCTGCTCTGTGGACACCTGTAAGCTCCTGGATTTCCAGGAGTT
CACCTTGTACTTGAGTACAAGAAAGATTGAAGGAGCCCGATCAGTGCTCAGACTGGAAAAGATCATGGAA
AACTTGAAGAATGCAGAGATTGAACCAGATGATTACTTTATGAGTCGCTATGAGAAGAAGCTAGAAGAAC
TCAAAGCTAAGGAGCAGTCAGGAACCCAGATAAGAAAGCCATCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC227897 representing NM_001145464
Red=Cloning site Green=Tags(s)

MAIKSIASRLRGSRRFLSGFVAGAVVGAAGAGLAALQFFRSQGAEGALTGKQPDGDADRKHCKFKPDPNI
PPTFSAFNEDYVGSGWSRGHMAPAGNNKFSSKAMAETFYLSNIVPQDFDNNSGYWNRIEMYCRELTERFE
DVWVVSGPLTLPQTRGDGKKIVSYQVIGEDNVAVPSHLYKVILARRSSVSTEPLALGAFVVPNEAIGFQP
QLTEFQVSLQDLEKLSGLVFFPHLDRTSDIRNICSVDTCKLLDFQEFTLYLSTRKIEGARSVLRLEKIME
NLKNAEIEPDDYFMSRYEKKLEELKAKEQSGTQIRKPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001145464
ORF Size 954 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001145464.1, NP_001138936.1
RefSeq ORF 957 bp
Locus ID 9941
UniProt ID Q9Y2C4
Protein Families Druggable Genome, Transmembrane
Protein Pathways Apoptosis
MW 35.44 kDa
Gene Summary This gene encodes an endo/exonuclease with 5'-3' exonuclease activity. The encoded enzyme catalyzes the hydrolysis of ester linkages at the 5' end of a nucleic acid chain. This enzyme is localized to the mitochondria and may play a role in programmed cell death. Alternatively spliced transcript variants have been described. A pseudogene exists on chromosome 18. [provided by RefSeq, Feb 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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