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PDE2A (NM_001146209) Human Tagged ORF Clone
CAT＃: RC228618
- TrueORF®
PDE2A (Myc-DDK-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4
ORF Plasmid: tGFP
"NM_001146209" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGS-PDE; cGSPDE; PDE2A1; PED2A4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228618 representing NM_001146209
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAACAGAGGATCCAGGAGGGGAAATCGCTTGCCCACAGGCGGGGCCAGCAGGTCTTCCTCAAGC CGGACGAGCCGCCGCCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGACGCCTTGCTGAGTCTGGGCTC TGTCATCGACATTTCAGGCCTGCAACGTGCTGTCAAGGAGGCCCTGTCAGCTGTGCTCCCCCGAGTGGAA ACTGTCTACACCTACCTACTGGATGGTGAGTCCCAGCTGGTGTGTGAGGACCCCCCACATGAGCTGCCCC AGGAGGGGAAAGTCCGGGAGGCTATCATCTCCCAGAAGCGGCTGGGCTGCAATGGGCTGGGCTTCTCAGA CCTGCCAGGGAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCTCCTGATACCCAAGTGCTGGTCATGCCG CTAGCGGACAAGGAGGCTGGGGCCGTGGCAGCTGTCATCTTGGTGCACTGTGGCCAGCTGAGTGATAATG AGGAATGGAGCCTGCAGGCGGTGGAGAAGCATACCCTGGTCGCCCTGCGGAGGGTGCAGGTCCTGCAGCA GCGCGGGCCCAGGGAGGCTCCCCGAGCCGTCCAGAACCCCCCGGAGGGGACGGCGGAAGACCAGAAGGGC GGGGCGGCGTACACCGACCGCGACCGCAAGATCCTCCAACTGTGCGGGGAACTCTACGACCTGGATGCCT CTTCCCTGCAGCTCAAAGTGCTCCAATACCTGCAGCAGGAGACCCGGGCATCCCGCTGCTGCCTCCTGCT GGTGTCGGAGGACAATCTCCAGCTTTCTTGCAAGGTCATCGGAGACAAAGTGCTCGGGGAAGAGGTCAGC TTTCCCTTGACAGGATGCCTGGGCCAGGTGGTGGAAGACAAGAAGTCCATCCAGCTGAAGGACCTCACCT CCGAGGATGTACAACAGCTGCAGAGCATGTTGGGCTGTGAGCTGCAGGCCATGCTCTGTGTCCCTGTCAT CAGCCGGGCCACTGACCAGGTGGTGGCCTTGGCCTGCGCCTTCAACAAGCTAGAAGGAGACTTGTTCACC GACGAGGACGAGCATGTGATCCAGCACTGCTTCCACTACACCAGCACCGTGCTCACCAGCACCCTGGCCT TCCAGAAGGAACAGAAACTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCAAAGAACCTCTTCACCCA CCTGGATGACGTCTCTGTCCTGCTCCAGGAGATCATCACGGAGGCCAGAAACCTCAGCAACGCAGAGATC TGCTCTGTGTTCCTGCTGGATCAGAATGAGCTGGTGGCCAAGGTGTTCGACGGGGGCGTGGTGGATGATG AGAGCTATGAGATCCGCATCCCGGCCGATCAGGGCATCGCGGGACACGTGGCGACCACGGGCCAGATCCT GAACATCCCTGACGCATATGCCCATCCGCTTTTCTACCGCGGCGTGGACGACAGCACCGGCTTCCGCACG CGCAACATCCTCTGCTTCCCCATCAAGAACGAGAACCAGGAGGTCATCGGTGTGGCCGAGCTGGTGAACA AGATCAATGGGCCATGGTTCAGCAAGTTCGACGAGGACCTGGCGACGGCCTTCTCCATCTACTGCGGCAT CAGCATCGCCCATTCTCTCCTATACAAAAAAGTGAATGAGGCTCAGTATCGCAGCCACCTGGCCAATGAG ATGATGATGTACCACATGAAGGTCTCCGATGATGAGTATACCAAACTTCTCCATGATGGGATCCAGCCTG TGGCTGCCATTGACTCCAATTTTGCAAGTTTCACCTATACCCCTCGTTCCCTGCCCGAGGATGACACGTC CATGGCCATCCTGAGCATGCTGCAGGACATGAATTTCATCAACAACTACAAAATTGACTGCCCGACCCTG GCCCGGTTCTGTTTGATGGTGAAGAAGGGCTACCGGGATCCCCCCTACCACAACTGGATGCACGCCTTTT CTGTCTCCCACTTCTGCTACCTGCTCTACAAGAACCTGGAGCTCACCAACTACCTCGAGGACATCGAGAT CTTTGCCTTGTTTATTTCCTGCATGTGTCATGACCTGGACCACAGAGGCACAAACAACTCTTTCCAGGTG GCCTCGAAATCTGTGCTGGCTGCGCTCTACAGCTCTGAGGGCTCCGTCATGGAGAGGCACCACTTTGCTC AGGCCATCGCCATCCTCAACACCCACGGCTGCAACATCTTTGATCATTTCTCCCGGAAGGACTATCAGCG CATGCTGGATCTGATGCGGGACATCATCTTGGCCACAGACCTGGCCCACCATCTCCGCATCTTCAAGGAC CTCCAGAAGATGGCTGAGGTGGGCTACGACCGAAACAACAAGCAGCACCACAGACTTCTCCTCTGCCTCC TCATGACCTCCTGTGACCTCTCTGACCAGACCAAGGGCTGGAAGACTACGAGAAAGATCGCGGAGCTGAT CTACAAAGAATTCTTCTCCCAGGGAGACCTGGAGAAGGCCATGGGCAACAGGCCGATGGAGATGATGGAC CGGGAGAAGGCCTATATCCCTGAGCTGCAAATCAGCTTCATGGAGCACATTGCAATGCCCATCTACAAGC TGTTGCAGGACCTGTTCCCCAAAGCGGCAGAGCTGTACGAGCGCGTGGCCTCCAACCGTGAGCACTGGAC CAAGGTGTCCCACAAGTTCACCATCCGCGGCCTCCCAAGTAACAACTCGCTGGACTTCCTGGATGAGGAG TACGAGGTGCCTGATCTGGATGGCACTAGGGCCCCCATCAATGGCTGCTGCAGCCTTGATGCTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228618 representing NM_001146209
Red=Cloning site Green=Tags(s) MKKQRIQEGKSLAHRRGQQVFLKPDEPPPPPQPCADSLQDALLSLGSVIDISGLQRAVKEALSAVLPRVE TVYTYLLDGESQLVCEDPPHELPQEGKVREAIISQKRLGCNGLGFSDLPGKPLARLVAPLAPDTQVLVMP LADKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALRRVQVLQQRGPREAPRAVQNPPEGTAEDQKG GAAYTDRDRKILQLCGELYDLDASSLQLKVLQYLQQETRASRCCLLLVSEDNLQLSCKVIGDKVLGEEVS FPLTGCLGQVVEDKKSIQLKDLTSEDVQQLQSMLGCELQAMLCVPVISRATDQVVALACAFNKLEGDLFT DEDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEI CSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRT RNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANE MMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTL ARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHRGTNNSFQV ASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKD LQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMD REKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEE YEVPDLDGTRAPINGCCSLDAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146209 |
| ORF Size | 2796 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146209.2, NP_001139681.1 |
| RefSeq Size | 4196 bp |
| RefSeq ORF | 2799 bp |
| Locus ID | 5138 |
| UniProt ID | O00408 |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 105 kDa |
| Gene Summary | Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line) (PubMed:24705027).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228618L3 | Lenti-ORF clone of PDE2A (Myc-DDK-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4 |
CNY 9,030.00 |
|
| RC228618L4 | Lenti-ORF clone of PDE2A (mGFP-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4 |
CNY 9,030.00 |
|
| RG228618 | PDE2A (tGFP-tagged) - Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4 |
CNY 7,890.00 |
|
| SC327253 | PDE2A (untagged)-Human phosphodiesterase 2A cGMP-stimulated (PDE2A) transcript variant 4 |
CNY 18,050.00 |
