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Prominin 2 (PROM2) (NM_001165978) Human Tagged ORF Clone
CAT＃: RC229025
PROM2 (Myc-DDK-tagged)-Human prominin 2 (PROM2), transcript variant 1
ORF Plasmid: tGFP
"NM_001165978" in other vectors (6)
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CNY 9,160.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PROML2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229025 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCACACACTGGCTCTGCTGGCTCCCCTGCTGGGCCTGGGCCTGGGGCTGGCCCTGAGTCAGCTGG CTGCAGGGGCCACAGACTGCAAGTTCCTTGGCCCGGCAGAGCACCTGACATTCACCCCAGCAGCCAGGGC CCGGTGGCTGGCCCCTCGAGTTCGTGCGCCAGGACTCCTGGACTCCCTCTATGGCACCGTGCGCCGCTTC CTCTCGGTGGTGCAGCTCAATCCTTTCCCTTCAGAGTTGGTAAAGGCCCTACTGAATGAGCTGGCCTCCG TGAAGGTGAATGAGGTGGTGCGGTACGAGGCGGGCTACGTGGTATGCGCTGTGATCGCGGGCCTCTACCT GCTGCTGGTGCCCACTGCCGGGCTTTGCTTCTGCTGCTGCCGCTGCCACCGGCGCTGCGGGGGACGAGTG AAGACAGAGCACAAGGCGCTGGCCTGTGAGCGCGCGGCCCTCATGGTCTTCCTGCTGCTGACCACCCTCT TGCTGCTGATTGGTGTGGTCTGTGCCTTTGTCACCAACCAGCGCACGCATGAACAGATGGGCCCCAGCAT CGAGGCCATGCCTGAGACCCTGCTCAGCCTCTGGGGCCTGGTCTCTGATGTCCCCCAAGAGCTGCAGGCC GTGGCACAGCAATTCTCCCTGCCCCAGGAGCAAGTCTCAGAGGAGCTGGATGGTGTTGGTGTGAGCATTG GGAGCGCGATCCACACTCAGCTCAGGAGCTCCGTGTACCCCTTGCTGGCGGCCGTGGGCAGTTTGGGCCA GGTCCTGCAGGTCTCCGTGCACCACCTGCAAACCTTGAATGCTACAGTGGTAGAGCTGCAGGCCGGGCAG CAGGACCTGGAGCCAGCCATCCGGGAACACCGGGACCGCCTCCTTGAGCTGCTGCAGGAGGCCAGGTGCC AGGGAGATTGTGCAGGGGCCCTGAGCTGGGCCCGCACCCTGGAGCTGGGTGCTGACTTCAGCCAGGTGCC CTCTGTGGACCATGTCCTGCACCAGCTAAAAGGTGTCCCCGAGGCCAACTTCTCCAGCATGGTCCAGGAG GAGAACAGCACCTTCAACGCCCTTCCAGCCCTGGCTGCCATGCAGACATCCAGCGTGGTGCAAGAGCTGA AGAAGGCAGTGGCCCAGCAGCCGGAAGGGGTGAGGACACTGGCTGAAGGGTTCCCGGGCTTGGAGGCAGC TTCCCGCTGGGCCCAGGCACTGCAGGAGGTGGAGGAGAGCAGCCGCCCCTACCTGCAGGAGGTGCAGAGA TACGAGACCTACAGGTGGATCGTGGGCTGCGTGCTGTGCTCCGTGGTCCTATTCGTGGTGCTCTGCAACC TGCTGGGCCTCAATCTGGGCATCTGGGGCCTGTCTGCCAGGGACGACCCCAGCCACCCAGAAGCCAAGGG CGAGGCTGGAGCCCGCTTCCTCATGGCAGGTGTGGGCCTCAGCTTCCTCTTTGCTGCACCCCTCATCCTC CTGGTGTTCGCCACCTTCCTGGTGGGTGGCAACGTGCAGACGCTGGTGTGCCGGAGCTGGGAGAACGGCG AGCTCTTTGAGTTTGCAGACACCCCAGGGAACCTGCCCCCGTCCATGAACCTGTCGCAACTTCTTGGCCT GAGGAAGAACATCAGCATCCACCAAGCCTATCAGCAGTGCAAGGAAGGGGCAGCGCTCTGGACAGTCCTG CAGCTCAACGACTCCTACGACCTGGAGGAGCACCTGGATATCAACCAGTATACCAACAAGCTACGGCAGG AGTTGCAGAGCCTGAAAGTAGACACACAGAGCCTGGACCTGCTGAGCTCAGCCGCCCGCCGGGACCTGGA GGCCCTGCAGAGCAGTGGGCTTCAGCGCATCCACTACCCCGACTTCCTCGTTCAGATCCAGAGGCCCGTG GTGAAGACCAGCATGGAGCAGCTGGCCCAGGAGCTGCAAGGACTGGCCCAGGCCCAAGACAATTCTGTGC TGGGGCAGCGGCTGCAGGAGGAGGCCCAAGGACTCAGAAACCTTCACCAGGAGAAGGTCGTCCCCCAGCA GAGCCTTGTGGCAAAGCTCAACCTCAGCGTCAGGGCCCTGGAGTCCTCTGCCCCGAATCTCCAGCTGGAG ACCTCAGATGTCCTAGCCAATGTCACCTACCTGAAAGGAGAGCTGCCTGCCTGGGCAGCCAGGATCCTGA GGAATGTGAGTGAGTGTTTCCTGGCCCGGGAGATGGGCTACTTCTCCCAGTACGTGGCCTGGGTGAGAGA GGAGGTGACTCAGCGCATTGCCACCTGCCAGCCCCTCTCCGGAGCCCTGGACAACAGCCGTGTGATCCTG TGTGACATGATGGCTGACCCCTGGAATGCCTTCTGGTTCTGCCTGGCATGGTGCACCTTCTTCCTGATCC CCAGCATCATCTTTGCCGTCAAGACCTCCAAATACTTCCGTCCTATCCGGAAACGCCTCAGCTCCACCAG CTCTGAGGAGACTCAGCTCTTCCACATCCCCCGGGTTACCTCCCTGAAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229025 protein sequence
Red=Cloning site Green=Tags(s) MKHTLALLAPLLGLGLGLALSQLAAGATDCKFLGPAEHLTFTPAARARWLAPRVRAPGLLDSLYGTVRRF LSVVQLNPFPSELVKALLNELASVKVNEVVRYEAGYVVCAVIAGLYLLLVPTAGLCFCCCRCHRRCGGRV KTEHKALACERAALMVFLLLTTLLLLIGVVCAFVTNQRTHEQMGPSIEAMPETLLSLWGLVSDVPQELQA VAQQFSLPQEQVSEELDGVGVSIGSAIHTQLRSSVYPLLAAVGSLGQVLQVSVHHLQTLNATVVELQAGQ QDLEPAIREHRDRLLELLQEARCQGDCAGALSWARTLELGADFSQVPSVDHVLHQLKGVPEANFSSMVQE ENSTFNALPALAAMQTSSVVQELKKAVAQQPEGVRTLAEGFPGLEAASRWAQALQEVEESSRPYLQEVQR YETYRWIVGCVLCSVVLFVVLCNLLGLNLGIWGLSARDDPSHPEAKGEAGARFLMAGVGLSFLFAAPLIL LVFATFLVGGNVQTLVCRSWENGELFEFADTPGNLPPSMNLSQLLGLRKNISIHQAYQQCKEGAALWTVL QLNDSYDLEEHLDINQYTNKLRQELQSLKVDTQSLDLLSSAARRDLEALQSSGLQRIHYPDFLVQIQRPV VKTSMEQLAQELQGLAQAQDNSVLGQRLQEEAQGLRNLHQEKVVPQQSLVAKLNLSVRALESSAPNLQLE TSDVLANVTYLKGELPAWAARILRNVSECFLAREMGYFSQYVAWVREEVTQRIATCQPLSGALDNSRVIL CDMMADPWNAFWFCLAWCTFFLIPSIIFAVKTSKYFRPIRKRLSSTSSEETQLFHIPRVTSLKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001165978 |
| ORF Size | 2502 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001165978.3 |
| RefSeq Size | 4739 bp |
| RefSeq ORF | 2505 bp |
| Locus ID | 150696 |
| UniProt ID | Q8N271 |
| Protein Families | Transmembrane |
| MW | 91.9 kDa |
| Gene Summary | This gene encodes a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229025L1 | Lenti ORF clone of Human prominin 2 (PROM2), transcript variant 1, Myc-DDK-tagged |
CNY 11,560.00 |
|
| RC229025L2 | Lenti ORF clone of Human prominin 2 (PROM2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC229025L3 | Lenti ORF clone of Human prominin 2 (PROM2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC229025L4 | Lenti ORF clone of Human prominin 2 (PROM2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG229025 | PROM2 (tGFP-tagged) - Human prominin 2 (PROM2), transcript variant 1 |
CNY 10,760.00 |
|
| SC327660 | PROM2 (untagged)-Human prominin 2 (PROM2) transcript variant 1 |
CNY 7,220.00 |
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