STK35 (NM_080836) Human Tagged ORF Clone

CAT#: RC229271

STK35 (Myc-DDK-tagged)-Human serine/threonine kinase 35 (STK35)

ORF Plasmid: DDK tGFP



  "NM_080836" in other vectors (7)

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CNY 3,976.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-STK35 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CLIK1; STK35L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC229271 representing NM_080836
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCACCAGGAGTCTCCGCTGGCCCGGGCGCCGGCGGGAGGTGCAGCTTATGTAAAGAGGTTATGTA
AAGGGCTCAGCTGGCGCGAACACGTGGAAAGCCACGGGAGCCTAGGAGCCCAGGCTTCCCCAGCGAGCGC
CGCGGCAGCAGAAGGATCCGCTACACGCCGGGCTCGGGCCGCCACCTCCCGCGCTGCTCGGTCCCGGAGG
CAGCCCGGGCCCGGAGCGGACCATCCCCAGGCAGGGGCTCCAGGGGGGAAACGGGCCGCCCGGAAGTGGA
GGTGCGCGGGCCAGGTCACAATCCAAGGTCCGGCTCCTCCGCGTCCCAGGGCCGGACGGAGGGATGAGGC
AGGGGGGGCCCGGGCAGCGCCGTTGCTGCTCCCCCCGCCGCCCGCAGCCATGGAAACGGGGAAGGACGGC
GCCCGCAGAGGTACACAAAGCCCGGAGCGGAAAAGGCGAAGCCCAGTGCCGCGGGCGCCCAGCACGAAGC
TGAGGCCGGCGGCGGCGGCCCGGGCCATGGATCCGGTGGCGGCCGAGGCCCCGGGCGAGGCCTTCCTGGC
GCGGCGACGGCCTGAGGGCGGTGGCGGGTCCGCGCGGCCGCGTTACAGCCTGTTGGCGGAGATCGGGCGC
GGCAGCTACGGCGTGGTTTATGAGGCAGTGGCCGGGCGCAGCGGGGCCCGGGTGGCGGTCAAGAAGATCC
GCTGCGACGCCCCCGAGAACGTGGAGCTGGCGCTGGCTGAATTCTGGGCCCTCACCAGCCTCAAGCGGCG
CCACCAGAACGTCGTGCAGTTTGAGGAGTGCGTCCTGCAGCGCAATGGGTTAGCCCAGCGCATGAGTCAC
GGCAACAAGAGCTCGCAGCTTTACCTGCGCCTGGTGGAGACCTCGCTGAAAGGAGAAAGGATCCTGGGTT
ATGCTGAGGAGCCCTGCTATCTCTGGTTTGTCATGGAGTTCTGTGAAGGTGGAGACCTGAATCAGTATGT
CCTGTCCCGGAGGCCAGACCCAGCCACCAACAAAAGTTTCATGCTACAGCTGACGAGCGCCATTGCCTTC
CTGCACAAAAACCATATTGTGCACAGGGACCTGAAGCCAGACAACATCCTCATCACAGAGCGGTCTGGCA
CCCCCATCCTCAAAGTGGCCGACTTTGGACTAAGCAAGGTCTGTGCTGGGCTGGCACCCCGAGGCAAAGA
GGGCAATCAAGACAACAAAAATGTGAATGTGAATAAGTACTGGCTGTCCTCAGCCTGCGGTTCGGACTTC
TACATGGCTCCTGAAGTCTGGGAGGGACACTACACAGCCAAGGCGGACATCTTTGCCCTGGGCATTATCA
TCTGGGCAATGATAGAAAGAATCACTTTTATTGACTCTGAGACCAAGAAGGAGCTCCTGGGGACCTACAT
TAAACAGGGGACTGAGATCGTCCCTGTTGGTGAGGCGCTGCTAGAAAACCCAAAGATGGAGTTGCACATC
CCCCAAAAACGCAGGACTTCCATGTCTGAGGGGATCAAGCAGCTCTTGAAAGATATGTTAGCTGCTAACC
CACAGGACCGGCCTGATGCCTTTGAACTTGAAACCAGAATGGACCAGGTCACATGTGCTGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC229271 representing NM_080836
Red=Cloning site Green=Tags(s)

MGHQESPLARAPAGGAAYVKRLCKGLSWREHVESHGSLGAQASPASAAAAEGSATRRARAATSRAARSRR
QPGPGADHPQAGAPGGKRAARKWRCAGQVTIQGPAPPRPRAGRRDEAGGARAAPLLLPPPPAAMETGKDG
ARRGTQSPERKRRSPVPRAPSTKLRPAAAARAMDPVAAEAPGEAFLARRRPEGGGGSARPRYSLLAEIGR
GSYGVVYEAVAGRSGARVAVKKIRCDAPENVELALAEFWALTSLKRRHQNVVQFEECVLQRNGLAQRMSH
GNKSSQLYLRLVETSLKGERILGYAEEPCYLWFVMEFCEGGDLNQYVLSRRPDPATNKSFMLQLTSAIAF
LHKNHIVHRDLKPDNILITERSGTPILKVADFGLSKVCAGLAPRGKEGNQDNKNVNVNKYWLSSACGSDF
YMAPEVWEGHYTAKADIFALGIIIWAMIERITFIDSETKKELLGTYIKQGTEIVPVGEALLENPKMELHI
PQKRRTSMSEGIKQLLKDMLAANPQDRPDAFELETRMDQVTCAA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080836
ORF Size 1602 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_080836.4
RefSeq ORF 1605 bp
Locus ID 140901
UniProt ID Q8TDR2
Protein Families Druggable Genome, Protein Kinase
MW 57.9 kDa
Gene Summary The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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