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Meckelin (TMEM67) (NM_153704) Human Tagged ORF Clone
CAT＃: RC229402
- TrueORF®
TMEM67 (Myc-DDK-tagged)-Human transmembrane protein 67 (TMEM67), transcript variant 1
ORF Plasmid: tGFP
"NM_153704" in other vectors (5)
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CNY 10,928.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JBTS6; MECKELIN; MKS3; NPHP11; TNEM67 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229402 representing NM_153704
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGCGCGGTGGGGCTGGGGTGGCAATGGCGGTTTGGTCCCTCTTATCCGCCCGGGCCGTGACCG CGTTCCTTCTGTTGTTCCTCCCTCGCTTCTTACAGGCCCAGACCTTCTCTTTCCCTTTCCAGCAGCCGGA GAAGTGCGACAACAACCAGTACTTTGATATCTCCGCCCTCTCGTGTGTTCCTTGTGGAGCTAACCAGAGG CAAGATGCCCGAGGAACTTCATGTGTATGTCTACCAGGATTTCAGATGATCTCTAATAATGGAGGACCTG CTATTATTTGTAAAAAGTGCCCAGAAAACATGAAAGGTGTTACAGAAGATGGCTGGAACTGCATTTCTTG CCCTAGTGACTTAACTGCCGAAGGAAAATGTCACTGTCCCATTGGCCATATTTTAGTGGAAAGAGACATT AATGGAACATTGTTGTCTCAAGCAACTTGTGAGCTCTGTGATGGAAATGAAAACTCTTTTATGGTAGTAA ATGCTTTAGGAGACAGGTGCGTCCGATGTGAGCCAACATTTGTTAATACCAGCAGGTCCTGTGCATGTTC AGAACCTAACATTTTAACAGGGGGATTATGTTTCAGCAGCACAGGGAATTTTCCTCTACGTAGAATTTCA GCTGCACGTTATGGAGAAGTTGGCATGTCTTTAACTTCAGAATGGTTTGCAAAGTATTTGCAATCATCAG CAGCTGCATGTTGGGTATATGCCAATCTAACATCTTGTCAAGCTCTTGGAAATATGTGTGTGATGAACAT GAATTCTTACGACTTTGCCACATTTGATGCATGTGGACTATTTCAGTTTATCTTTGAAAATACTGCTGGA CTGAGCACTGTTCATTCTATTTCATTTTGGAGACAGAATCTTCCTTGGCTGTTTTATGGAGACCAGTTAG GATTAGCACCTCAAGTGCTCAGTTCTACCTCTCTTCCTACAAATTTCAGTTTTAAAGGAGAAAACCAGAA TACAAAACTGAAGTTTGTTGCTGCTTCCTATGATATAAGAGGAAATTTTCTCAAGTGGCAAACTTTAGAA GGAGGTGTTTTACAGCTTTGTCCAGACACAGAGACAAGGCTAAATGCTGCTTATTCATTTGGAACAACCT ACCAACAAAATTGTGAGATTCCTATCTCTAAGATCTTAATTGACTTTCCCACTCCTATATTTTATGATGT GTACCTTGAATATACTGATGAAAATCAACATCAATATATTTTGGCTGTGCCTGTGTTAAACCTAAATCTT CAACATAATAAGATATTTGTGAACCAAGACAGCAACTCTGGAAAGTGGCTTCTAACTCGGCGCATTTTCT TAGTGGATGCAGTAAGTGGACGAGAAAATGACTTAGGAACTCAGCCAAGAGTAATTCGAGTTGCTACTCA AATATCACTGAGTGTCCACCTTGTACCCAACACAATAAATGGAAACATCTACCCTCCCTTAATCACCATT GCCTACAGTGACATTGATATCAAAGATGCCAACAGCCAGTCTGTGAAGGTTTCTTTCTCAGTCACATATG AAATGGATCATGGAGAAGCACATGTCCAGACAGATATTGCTTTGGGTGTATTGGGTGGGCTAGCTGTTTT AGCATCTCTTTTGAAGACAGCAGGATGGAAGAGGCGCATTGGGAGTCCCATGATTGATTTACAGACAGTT GTGAAATTCTTGGTGTACTATGCTGGTGATCTGGCCAATGTTTTCTTTATCATCACAGTGGGAACAGGTC TTTACTGGCTTATTTTCTTCAAAGCACAGAAGTCTGTGTCTGTTTTGCTGCCAATGCCAATTCAGGAAGA ACGTTTTGTCACTTATGTTGGATGTGCCTTTGCTCTGAAGGCACTACAATTTTTGCATAAGCTCATATCC CAGATTACAATAGATGTATTCTTTATTGATTGGGAGCGACCTAAAGGAAAGGTTCTTAAAGCTGTTGAAG GTGAGGGTGGTGTACGAAGTGCCACTGTTCCTGTAAGCATATGGAGAACATATTTTGTAGCAAATGAATG GAATGAAATTCAGACTGTGAGAAAAATTAATTCACTCTTTCAAGTACTTACTGTCCTCTTCTTTTTGGAG GTTGTGGGATTCAAGAACTTAGCATTAATGGACTCATCTTCTAGTCTTTCTAGAAACCCACCTAGCTACA TAGCTCCTTATAGCTGCATTTTGAGATATGCAGTGTCTGCTGCTCTTTGGCTAGCCATTGGAATTATACA GGTCGTGTTCTTTGCTGTCTTTTATGAGAGATTTATAGAAGATAAAATTCGACAGTTCGTTGATTTATGC TCTATGAGTAATATATCAGTGTTTCTGTTATCCCACAAATGTTTTGGATATTACATTCATGGTAGATCAG TACATGGGCATGCAGATACTAATATGGAAGAAATGAATATGAACCTTAAAAGAGAAGCGGAAAATTTGTG TAGCCAGAGAGGTTTGGTACCCAACACAGATGGTCAGACTTTTGAGATTGCAATTTCTAACCAGATGAGA CAACATTATGACAGAATTCATGAGACACTAATAAGGAAAAATGGTCCTGCTAGACTACTGAGTTCATCAG CAAGTACTTTTGAGCAGAGTATAAAAGCATATCATATGATGAATAAATTTCTTGGCTCCTTCATTGACCA TGTTCATAAGGAAATGGATTACTTTATAAAAGATAAGTTGCTTCTTGAAAGAATTCTTGGAATGGAATTC ATGGAACCAATGGAAAAAAGCATCTTTTACAATGATGAAGGTTATTCTTTCAGCAGTGTCCTGTATTATG GAAATGAAGCTACTCTTCTTATTTTTGATCTGCTGTTCTTCTGTGTTGTGGATTTGGCTTGCCAAAATTT TATTTTAGCATCCTTCCTTACATATCTACAACAAGAGATTTTTAGATATATCCGTAATACAGTAGGACAA AAGAATTTGGCATCCAAAACATTGGTGGATCAAAGATTTTTGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229402 representing NM_153704
Red=Cloning site Green=Tags(s) MATRGGAGVAMAVWSLLSARAVTAFLLLFLPRFLQAQTFSFPFQQPEKCDNNQYFDISALSCVPCGANQR QDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDI NGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTFVNTSRSCACSEPNILTGGLCFSSTGNFPLRRIS AARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFENTAG LSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQTLE GGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTPIFYDVYLEYTDENQHQYILAVPVLNLNL QHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITI AYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTV VKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLIS QITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLE VVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLC SMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMR QHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEF MEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQ KNLASKTLVDQRFLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153704 |
| ORF Size | 2985 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153704.6 |
| RefSeq ORF | 2988 bp |
| Locus ID | 91147 |
| UniProt ID | Q5HYA8 |
| Protein Families | Transmembrane |
| MW | 111.6 kDa |
| Gene Summary | The protein encoded by this gene localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). [provided by RefSeq, Nov 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229402L3 | Lenti ORF clone of Human transmembrane protein 67 (TMEM67), transcript variant 1, Myc-DDK-tagged |
CNY 11,690.00 |
|
| RC229402L4 | Lenti ORF clone of Human transmembrane protein 67 (TMEM67), transcript variant 1, mGFP tagged |
CNY 11,690.00 |
|
| RG229402 | TMEM67 (tGFP-tagged) - Human transmembrane protein 67 (TMEM67), transcript variant 1 |
CNY 10,740.00 |
|
| SC100509 | TMEM67 (untagged)-Human transmembrane protein 67 (TMEM67), transcript variant 1 |
CNY 10,944.00 |
|
| SC328037 | TMEM67 (untagged)-Human transmembrane protein 67 (TMEM67) transcript variant 1 |
CNY 19,100.00 |
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