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GPR124 (ADGRA2) (NM_032777) Human Tagged ORF Clone
CAT＃: RC229431
GPR124 (Myc-DDK-tagged)-Human G protein-coupled receptor 124 (GPR124)
ORF Plasmid: tGFP
"NM_032777" in other vectors (5)
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CNY 13,728.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPR124; TEM5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229431 representing NM_032777
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCCGGGGGACGCAGGATGCGGGGGGCGCCCGCGCGCCTGCTGCTGCCGCTGCTGCCGTGGCTCC TGCTGCTCCTGGCGCCCGAGGCTCGGGGCGCGCCCGGCTGCCCGCTATCCATCCGCAGCTGCAAGTGCTC GGGGGAGCGGCCCAAGGGGCTGAGCGGCGGCGTCCCTGGCCCGGCTCGGCGGAGGGTGGTGTGCAGCGGC GGGGACCTCCCGGAGCCTCCCGAGCCCGGCCTTCTGCCTAACGGCACCGTTACCCTGCTCTTGAGCAATA ACAAGATCACGGGGCTCCGCAATGGCTCCTTCCTGGGACTGTCACTGCTGGAGAAGCTGGACCTGAGGAA CAACATCATCAGCACAGTGCAGCCGGGCGCCTTCCTGGGCCTGGGGGAGCTGAAGCGTTTAGATCTCTCC AACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATAT CTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTT GGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTG CAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCAGGCCCTGGGCAGCCTCCAGG AGGCCCAGCTCTGCTGCGAGGGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGT GGTGTTCCAGGGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGC TGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCC ACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTGTGGGCCTCAGGCGAGTGGGA GTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAGGTGGAGATCGTGGTGCTGGAGACCTCTGCC TCCTACTGCCCCGCCGAGCGTGTTGCCAACAACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCA TCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCAC CCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACC AACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGG CTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTAGTCTATGTGGC TCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGAGGTGATGGTGGACATG GCCAGCAACCTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCC GCATCGTGGGTGCCCTGGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAA TGCGAGGAACGTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCC TTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGC CCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTC CTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCGAGTCTATTCTCATCCCTTCCG GCTGCCCTGGCTCCCCCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCT TCCACAGCCACAGCAACACCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCC CGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGG CACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCT GGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGG CAATGTGGCCGTGCTCATGGAGCTGAGCGCCTTTCCCAGGGAGGTGGGGGGCGCCGGGGCAGGGCTGCAC CCCGTGGTATACCCCTGCACGGCCTTGCTGCTGCTCTGCCTCTTCGCCACCATCATCACCTACATCCTCA ACCACAGCTCCATCCGTGTGTCCCGGAAAGGCTGGCACATGCTGCTGAACTTGTGCTTCCACATAGCCAT GACCTCTGCTGTCTTTGCGGGGGGCATCACACTCACCAACTACCAGATGGTCTGCCAGGCGGTGGGCATC ACCCTGCACTACTCCTCCCTATCCACGCTGCTCTGGATGGGCGTGAAGGCGCGAGTGCTCCATAAGGAGC TCACCTGGAGGGCACCCCCTCCGCAAGAAGGGGACCCCGCTCTGCCTACTCCCAGTCCTATGCTCCGGTT CTATTTGATCGCTGGAGGGATTCCACTCATTATCTGTGGCATCACAGCTGCAGTCAACATCCACAACTAC CGGGACCACAGCCCCTACTGCTGGCTGGTGTGGCGTCCAAGCCTTGGCGCCTTCTACATCCCTGTGGCTT TGATTCTGCTCATCACCTGGATCTATTTCCTGTGCGCCGGGCTACGCTTACGGGGTCCTCTGGCACAGAA CCCCAAGGCGGGCAACAGCAGGGCCTCCCTGGAGGCAGGGGAGGAGCTGAGGGGTTCCACCAGGCTCAGG GGCAGCGGCCCCCTCCTGAGTGACTCAGGTTCCCTTCTTGCTACTGGGAGCGCGCGAGTGGGGACGCCCG GGCCCCCGGAGGATGGTGACAGCCTCTATTCTCCGGGAGTCCAGCTAGGGGCGCTGGTGACCACGCACTT CCTGTACTTGGCCATGTGGGCCTGCGGGGCTCTGGCAGTGTCCCAGCGCTGGCTGCCCCGGGTGGTGTGC AGCTGCTTGTACGGGGTGGCAGCCTCCGCCCTGGGCCTCTTCGTCTTCACTCACCACTGTGCCAGGCGGA GGGACGTGAGAGCCTCGTGGCGCGCCTGCTGCCCCCCTGCCTCTCCCGCGGCCCCCCATGCCCCGCCCCG GGCCCTGCCCGCCGCCGCAGAGGACGGTTCCCCGGTGTTCGGGGAGGGCCCCCCCTCCCTCAAGTCCTCC CCAAGCGGCAGCAGCGGCCATCCGCTGGCTCTGGGCCCCTGCAAGCTCACCAACCTGCAGCTGGCCCAGA GTCAGGTGTGCGAGGCGGGGGCGGCGGCCGGCGGGGAAGGAGAGCCGGAGCCGGCGGGCACCCGGGGAAA CCTCGCCCACCGCCACCCCAACAACGTGCACCACGGGCGTCGGGCGCACAAGAGCCGGGCCAAGGGACAC CGCGCGGGGGAGGCCTGCGGCAAGAACCGGCTCAAGGCCCTGCGCGGGGGCGCGGCGGGGGCGCTGGAGC TGCTGTCCAGCGAGAGCGGCAGTCTGCACAACAGCCCCACCGACAGCTACCTGGGCAGCAGCCGCAACAG CCCGGGCGCCGGCCTGCAGCTGGAAGGCGAGCCCATGCTCACGCCGTCCGAGGGCAGCGACACCAGCGCC GCGCCGCTTTCTGAGGCGGGCCGGGCAGGCCAGCGCCGCAGCGCCAGCCGCGACAGTCTCAAGGGCGGCG GCGCGCTGGAGAAGGAGAGCCATCGCCGCTCGTACCCGCTCAACGCCGCCAGCCTAAACGGCGCCCCCAA GGGGGGCAAGTACGACGACGTCACCCTGATGGGCGCGGAGGTAGCCAGCGGCGGCTGCATGAAGACCGGA CTCTGGAAGAGCGAAACTACCGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229431 representing NM_032777
Red=Cloning site Green=Tags(s) MGAGGRRMRGAPARLLLPLLPWLLLLLAPEARGAPGCPLSIRSCKCSGERPKGLSGGVPGPARRRVVCSG GDLPEPPEPGLLPNGTVTLLLSNNKITGLRNGSFLGLSLLEKLDLRNNIISTVQPGAFLGLGELKRLDLS NNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSL QLSEHTLCAYPSALHAQALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIR WYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLETSA SYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDRAGRWEPGDYSHCLYT NDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDM ASNLMLVDEHLLWLAQREDKACSRIVGALERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTA FQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLP AALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLR HWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSAFPREVGGAGAGLH PVVYPCTALLLLCLFATIITYILNHSSIRVSRKGWHMLLNLCFHIAMTSAVFAGGITLTNYQMVCQAVGI TLHYSSLSTLLWMGVKARVLHKELTWRAPPPQEGDPALPTPSPMLRFYLIAGGIPLIICGITAAVNIHNY RDHSPYCWLVWRPSLGAFYIPVALILLITWIYFLCAGLRLRGPLAQNPKAGNSRASLEAGEELRGSTRLR GSGPLLSDSGSLLATGSARVGTPGPPEDGDSLYSPGVQLGALVTTHFLYLAMWACGALAVSQRWLPRVVC SCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPAAPHAPPRALPAAAEDGSPVFGEGPPSLKSS PSGSSGHPLALGPCKLTNLQLAQSQVCEAGAAAGGEGEPEPAGTRGNLAHRHPNNVHHGRRAHKSRAKGH RAGEACGKNRLKALRGGAAGALELLSSESGSLHNSPTDSYLGSSRNSPGAGLQLEGEPMLTPSEGSDTSA APLSEAGRAGQRRSASRDSLKGGGALEKESHRRSYPLNAASLNGAPKGGKYDDVTLMGAEVASGGCMKTG LWKSETTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032777 |
| ORF Size | 4014 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032777.9 |
| RefSeq ORF | 4017 bp |
| Locus ID | 25960 |
| UniProt ID | Q96PE1 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 142.5 kDa |
| Gene Summary | Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (PubMed:30026314). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229431L3 | Lenti ORF clone of Human G protein-coupled receptor 124 (GPR124), Myc-DDK-tagged |
CNY 15,200.00 |
|
| RC229431L4 | Lenti ORF clone of Human G protein-coupled receptor 124 (GPR124), mGFP tagged |
CNY 16,128.00 |
|
| RG229431 | GPR124 (tGFP-tagged) - Human G protein-coupled receptor 124 (GPR124) |
CNY 15,328.00 |
|
| SC305501 | GPR124 (untagged)-Human G protein-coupled receptor 124 (GPR124) |
CNY 25,650.00 |
|
| SC328066 | GPR124 (untagged)-Human G protein-coupled receptor 124 (GPR124) |
CNY 13,744.00 |
