MPP1 (NM_001166462) Human Tagged ORF Clone

CAT#: RC230084

  • TrueORF®

MPP1 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 4

ORF Plasmid: DDK tGFP



  "NM_001166462" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


MPP1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AAG12; DXS552E; EMP55; MRG1; PEMP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC230084 representing NM_001166462
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCTGGGCTGTATCGCATCCATTGAATACTGTGACCGAGGACATGTACACCAACGGGTCTCCTG
CCCCAGGTAGCCCTGCCCAGGTCAAGGGACAGGAGGTGCGGAAAGTGCGACTCATACAGTTTGAGAAGGT
CACAGAAGAGCCCATGGGAATCACGCTGAAGCTGAATGAAAAACAGTCCTGTACGGTGGCCAGAATTCTT
CATGGTGGCATGATCCATAGACAAGGCTCCCTTCACGTGGGGGATGAGATCCTAGAAATCAATGGCACAA
ATGTGACAAATCATTCAGTGGATCAGCTGCAGAAGGCGATGAAAGAAACCAAAGGAATGATCTCATTAAA
AGTAATTCCCAACCAGCAAAGCCGTCTTCCTGCACTACAGATGTTCATGAGAGCGCAGTTTGACTATGAT
CCCAAAAAGGACAATCTGATCCCTTGCAAGGAGGCGGGACTGAAGTTTGCTACTGGGGACATTATCCAGA
TTATCAACAAGGATGACAGCAATTGGTGGCAGGGACGGGTGGAAGGCTCCTCCAAGGAGTCAGCAGGATT
GATCCCTTCCCCTGAGCTGCAGGAATGGCGAGTGGCAAGTATGGCTCAGTCAGCTCCTAGCGAAGCCCCG
AGCTGCAGTCCCTTTGGGAAGAAGAAGAAGTACAAAGACAAATATCTGGCCAAGCACAGCTCGATTTTTG
ATCAGTTGGATGTTGTTTCCTACGAGGAAGTCGTTCGGCTCCCTGCATTCAAGAGGAAGACCCTGGTGCT
GATCGGAGCCAGTGGGGTGGGTCGCAGCCACATTAAGAATGCCCTGCTCAGCCAGAATCCGGAGAAGTTT
GTGTACCCTGTCCCATATACAACACGGCCGCCAAGGAAGAGTGAGGAAGATGGGAAGGAGTACCACTTTA
TCTCAACGGAGGAGATGACGAGGAACATCTCTGCCAATGAGTTCTTGGAGTTTGGCAGCTACCAAGGCAA
CATGTTTGGCACCAAATTTGAAACAGTGCACCAGATCCATAAGCAGAACAAGATTGCCATCCTTGACATT
GAGCCCCAGACCCTGAAAATTGTTCGGACAGCAGAACTTTCGCCTTTCATTGTGTTCATTGCACCTACTG
ACCAGGGCACTCAGACAGAAGCCCTGCAGCAGCTGCAGAAGGACTCTGAGGCCATCCGCAGCCAGTACGC
TCACTACTTTGACCTCTCACTGGTCAATAATGGTGTTGATGAAACCCTTAAGAAATTACAAGAAGCCTTC
GACCAAGCGTGCAGTTCTCCACAGTGGGTGCCTGTCTCCTGGGTTTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC230084 representing NM_001166462
Red=Cloning site Green=Tags(s)

MESWAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARIL
HGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYD
PKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAP
SCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKF
VYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDI
EPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAF
DQACSSPQWVPVSWVY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001166462
ORF Size 1308 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001166462.1, NP_001159934.1
RefSeq ORF 1311 bp
Locus ID 4354
UniProt ID Q00013
Protein Families Druggable Genome
MW 49.5 kDa
Gene Summary This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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