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ARHGEF9 (NM_001173479) Human Tagged ORF Clone
CAT＃: RC230127
ARHGEF9 (Myc-DDK-tagged)-Human Cdc42 guanine nucleotide exchange factor (GEF) 9 (ARHGEF9), transcript variant 2
ORF Plasmid: tGFP
"NM_001173479" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | COLLYBISTIN; DEE8; EIEE8; HPEM-2; PEM-2; PEM2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230127 representing NM_001173479
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGGATAAGGGGCGGATCGGGAATGCTCTGGGTGAACCAGGAGGATGAGGTGGAGGAGGGGCCCA GCGATGTGCAGAACGGACACCTGGACCCCAATTCAGACTGCCTCTGTCTGGGGCGGCCACTACAGAACCG GGACCAGATGCGGGCCAATGTCATCAATGAGATAATGAGCACTGAGCGTCACTACATCAAGCACCTCAAG GATATTTGTGAGGGCTATCTGAAGCAGTGCCGGAAGAGAAGGGACATGTTCAGTGACGAGCAACTGAAGG TAATCTTTGGGAACATTGAAGATATCTACAGATTTCAGATGGGCTTTGTGAGAGACCTGGAGAAACAGTA TAACAATGATGACCCCCACCTCAGCGAGATAGGACCCTGCTTCCTAGAGCACCAAGATGGATTCTGGATA TACTCTGAGTATTGTAACAACCACCTGGATGCTTGCATGGAGCTCTCCAAACTGATGAAGGACAGCCGCT ACCAGCACTTCTTTGAGGCCTGTCGCCTCTTGCAGCAGATGATTGACATTGCTATCGATGGTTTCCTTTT GACTCCAGTGCAGAAGATCTGCAAGTATCCCTTACAGTTGGCTGAGCTCCTAAAGTATACTGCCCAAGAC CACAGTGACTACAGGTATGTGGCAGCTGCTTTGGCTGTCATGAGAAATGTGACTCAGCAGATCAACGAAC GCAAGCGACGTTTAGAGAATATTGACAAGATTGCTCAGTGGCAGGCTTCTGTCCTAGACTGGGAGGGCGA GGACATCCTAGACAGGAGCTCGGAGCTGATCTACACTGGGGAGATGGCCTGGATCTACCAGCCCTACGGC CGCAACCAGCAGCGGGTCTTCTTCCTGTTTGACCACCAGATGGTCCTCTGCAAGAAGGACCTAATCCGGA GAGACATCCTGTACTACAAAGGCCGCATTGACATGGATAAATATGAGGTAGTTGACATTGAGGATGGCAG AGATGATGACTTCAATGTCAGCATGAAGAATGCCTTTAAGCTTCACAACAAGGAGACTGAGGAGATACAT CTGTTCTTTGCCAAGAAGCTGGAGGAAAAAATACGCTGGCTCAGGGCTTTCAGAGAAGAGAGGAAAATGG TACAGGAAGATGAAAAAATTGGCTTTGAAATTTCTGAAAACCAGAAGAGGCAGGCTGCAATGACTGTGAG AAAAGTCCCTAAGCAAAAAGGTGTCAACTCTGCCCGCTCAGTTCCTCCTTCCTACCCACCACCGCAGGAC CCGTTAAACCACGGCCAGTACCTGGTCCCCGACGGCATCGCTCAGTCGCAGGTCTTTGAGTTCACCGAAC CCAAGCGCAGCCAGTCACCATTCTGGCAAAACTTCAGCAGGTTAACCCCCTTCAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230127 representing NM_001173479
Red=Cloning site Green=Tags(s) MQWIRGGSGMLWVNQEDEVEEGPSDVQNGHLDPNSDCLCLGRPLQNRDQMRANVINEIMSTERHYIKHLK DICEGYLKQCRKRRDMFSDEQLKVIFGNIEDIYRFQMGFVRDLEKQYNNDDPHLSEIGPCFLEHQDGFWI YSEYCNNHLDACMELSKLMKDSRYQHFFEACRLLQQMIDIAIDGFLLTPVQKICKYPLQLAELLKYTAQD HSDYRYVAAALAVMRNVTQQINERKRRLENIDKIAQWQASVLDWEGEDILDRSSELIYTGEMAWIYQPYG RNQQRVFFLFDHQMVLCKKDLIRRDILYYKGRIDMDKYEVVDIEDGRDDDFNVSMKNAFKLHNKETEEIH LFFAKKLEEKIRWLRAFREERKMVQEDEKIGFEISENQKRQAAMTVRKVPKQKGVNSARSVPPSYPPPQD PLNHGQYLVPDGIAQSQVFEFTEPKRSQSPFWQNFSRLTPFKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001173479 |
| ORF Size | 1389 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001173479.2 |
| RefSeq ORF | 1392 bp |
| Locus ID | 23229 |
| UniProt ID | O43307 |
| MW | 55.4 kDa |
| Gene Summary | The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. This brain-specific protein also acts as an adaptor protein for the recruitment of gephyrin and together these proteins facilitate receceptor recruitement in GABAnergic and glycinergic synapses. Defects in this gene are the cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy, as well as several other types of cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230127L3 | Lenti-ORF clone of ARHGEF9 (Myc-DDK-tagged)-Human Cdc42 guanine nucleotide exchange factor (GEF) 9 (ARHGEF9), transcript variant 2 |
CNY 5,890.00 |
|
| RC230127L4 | Lenti-ORF clone of ARHGEF9 (mGFP-tagged)-Human Cdc42 guanine nucleotide exchange factor (GEF) 9 (ARHGEF9), transcript variant 2 |
CNY 5,890.00 |
|
| RG230127 | ARHGEF9 (tGFP-tagged) - Human Cdc42 guanine nucleotide exchange factor (GEF) 9 (ARHGEF9), transcript variant 2 |
CNY 5,256.00 |
|
| SC328765 | ARHGEF9 (untagged)-Human Cdc42 guanine nucleotide exchange factor (GEF) 9 (ARHGEF9) transcript variant 2 |
CNY 7,410.00 |
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