SP110 (NM_001185015) Human Tagged ORF Clone

CAT#: RC230264

SP110 (Myc-DDK-tagged)-Human SP110 nuclear body protein (SP110), transcript variant d

ORF Plasmid: DDK tGFP



  "NM_001185015" in other vectors (4)

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CNY 4,136.00

CNY 4,750.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SP110 mouse monoclonal antibody, clone OTI4C1 (formerly 4C1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms IFI41; IFI75; IPR1; VODI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC230264 representing NM_001185015
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGGGGCTTCAGGATGTTCACCATGACAAGAGCCATGGAAGAGGCTCTTTTTCAGCACTTCATGC
ACCAGAAGCTGGGGATCGCCTATGCCATACACAAGCCATTTCCCTTCTTTGAAGGCCTCCTAGACAACTC
CATCATCACTAAGAGAATGTACATGGAATCTCTGGAAGCCTGTAGAAATTTGATCCCTGTATCCAGAGTG
GTGCACAACATTCTCACCCAACTGGAGAGGACTTTTAACCTGTCTCTTCTGGTGACATTGTTCAGTCAAA
TTAACCTGCGTGAATATCCCAATCTGGTGACGATTTACAGAAGCTTCAAACGTGTTGGTGCTTCCTATGA
ATGGCAGAGCAGAGACACACCAATCCTACTTGAAGCCCCAACTGGCCTAGCAGAAGGAAGCTCCCTCCAT
ACCCCACTGGCGCTGCCCCCACCACAACCCCCTCAACCAAGCTGTTCACCCTGTGCGCCAAGAGTCAGTG
AGCCTGGAACATCCTCCCAGCAAAGCGATGAGATCCTGAGTGAGTCGCCCAGCCCATCTGACCCTGTCCT
GCCTCTCCCTGCACTCATCCAGGAAGGAAGAAGCACTTCAGTGACCAATGACAAGTTAACATCCAAAATG
AATGCGGAAGAAGACTCAGAAGAGATGCCCAGCCTCCTCACTAGCACTGTGCAAGTGGCCAGTGACAACC
TGATCCCCCAAATAAGAGATAAAGAAGACCCTCAAGAGATGCCCCACTCTCCCTTGGGCTCTATGCCAGA
GATAAGAGATAATTCTCCAGAACCAAATGACCCAGAAGAGCCCCAGGAGGTGTCCAGCACACCTTCAGAC
AAGAAAGGAAAGAAAAGAAAAAGATGTATCTGGTCAACTCCAAAAAGGAGACATAAGAAAAAAAGCCTCC
CAGGAGGGACAGCCTCATCTAGACACGGAATCCAAAAGAAGCTCAAAAGGGTGGATCAGGTTCCTCAAAA
GAAAGATGACTCAACTTGTAACTCCACGGTAGAGACAAGGGCCCAAAAGGCGAGAACTGAATGTGCCCGA
AAGTCGAGATCAGAGGAGATCATTGATGGCACTTCAGAAATGAATGAAGGAAAGAGGTCCCAGAAGACGC
CTAGTACACCACGAAGGGTCACACAAGGGGCAGCCTCACCTGGGCATGGCATCCAAGAGAAGCTCCAAGT
GGTGGATAAGGTGACTCAAAGGAAAGACGACTCAACCTGGAACTCAGAGGTCATGATGAGGGTCCAAAAG
GCAAGAACTAAATGTGCCCGAAAGTCCAGATTGAAAGAAAAGAAAAAGGAGAAAGATATCTGTTCAAGCT
CAAAAAGGAGATTTCAGAAAAATATTCACCGAAGAGGAAAACCCAAAAGTGACACTGTGGATTTTCACTG
TTCTAAGCTCCCCGTGACCTGTGGTGAGGCGAAAGGGATTTTATATAAGAAGAAAATGAAACACGGATCC
TCAGTGAAGTGCATTCGGAATGAGGATGGAACTTGGTTAACACCAAATGAATTTGAAGTCGAAGGAAAAG
GAAGGAACGCAAAGAACTGGAAACGGAATATACGTTGTGAAGGAATGACCCTAGGAGAGCTGCTGAAGAG
TGGACTTTTGCTCTGTCCTCCAAGAATAAATCTCAAGAGAGAGTTAAATAGCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC230264 representing NM_001185015
Red=Cloning site Green=Tags(s)

MGRGFRMFTMTRAMEEALFQHFMHQKLGIAYAIHKPFPFFEGLLDNSIITKRMYMESLEACRNLIPVSRV
VHNILTQLERTFNLSLLVTLFSQINLREYPNLVTIYRSFKRVGASYEWQSRDTPILLEAPTGLAEGSSLH
TPLALPPPQPPQPSCSPCAPRVSEPGTSSQQSDEILSESPSPSDPVLPLPALIQEGRSTSVTNDKLTSKM
NAEEDSEEMPSLLTSTVQVASDNLIPQIRDKEDPQEMPHSPLGSMPEIRDNSPEPNDPEEPQEVSSTPSD
KKGKKRKRCIWSTPKRRHKKKSLPGGTASSRHGIQKKLKRVDQVPQKKDDSTCNSTVETRAQKARTECAR
KSRSEEIIDGTSEMNEGKRSQKTPSTPRRVTQGAASPGHGIQEKLQVVDKVTQRKDDSTWNSEVMMRVQK
ARTKCARKSRLKEKKKEKDICSSSKRRFQKNIHRRGKPKSDTVDFHCSKLPVTCGEAKGILYKKKMKHGS
SVKCIRNEDGTWLTPNEFEVEGKGRNAKNWKRNIRCEGMTLGELLKSGLLLCPPRINLKRELNSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001185015
ORF Size 1665 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001185015.2
RefSeq ORF 1668 bp
Locus ID 3431
UniProt ID Q9HB58
Protein Families Druggable Genome, Transcription Factors
MW 63.1 kDa
Gene Summary The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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