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ZFX (NM_001178086) Human Tagged ORF Clone
CAT＃: RC230300
ZFX (Myc-DDK-tagged)-Human zinc finger protein, X-linked (ZFX), transcript variant 4
ORF Plasmid: tGFP
"NM_001178086" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ZNF926 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230300 representing NM_001178086
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGACACAGAGTCGGAAATTGATCCTTGTAAAGTGGATGGCACTTGCCCTGAGGTCATCAAGG TGTACATTTTTAAAGCTGACCCTGGAGAAGATGACTTAGGTGGAACTGTAGACATTGTGGAGAGTGAGCC TGAGAATGATCATGGAGTTGAACTGCTTGATCAGAACAGCAGTATTCGTGTTCCCAGGGAAAAGATGGTT TATATGACTGTCAATGACTCTCAGCCAGAAGATGAAGATTTAAATGTTGCTGAAATCGCTGACGAAGTTT ATATGGAAGTGATCGTAGGAGAGGAGGATGCTGCAGCAGCAGCGGCAGCCGCCGCCGTGCACGAGCAGCA AATGGATGACAATGAAATCAAAACCTTCATGCCGATTGCATGGGCAGCAGCTTATGGTAATAATTCTGAT GGAATTGAAAACCGGAATGGCACTGCAAGTGCCCTCTTGCACATAGATGAGTCTGCTGGCCTCGGCAGAC TGGCTAAACAAAAACCAAAGAAAAGGAGAAGACCTGATTCCAGGCAGTACCAAACAGCAATAATTATTGG CCCTGATGGACATCCTTTGACTGTCTATCCTTGCATGATTTGTGGGAAGAAGTTTAAGTCGAGAGGTTTT TTGAAAAGGCACATGAAAAACCATCCCGAACACCTTGCCAAGAAGAAATACCGCTGTACTGACTGTGATT ACACTACCAACAAGAAGATAAGTTTACACAACCACCTGGAGAGCCACAAGCTGACCAGCAAGGCAGAGAA GGCCATTGAATGCGATGAGTGTGGGAAGCATTTCTCTCATGCAGGGGCTTTGTTTACTCACAAAATGGTG CATAAGGAAAAAGGAGCCAACAAAATGCACAAGTGTAAATTCTGTGAATACGAGACAGCTGAACAAGGGT TATTGAATCGCCACCTCTTGGCAGTCCACAGCAAGAACTTTCCTCATATTTGTGTGGAGTGTGGTAAGGG TTTTCGTCACCCGTCAGAGCTCAAAAAGCACATGAGAATCCATACTGGGGAGAAGCCGTACCAATGCCAG TACTGCGAATATAGGTCTGCAGACTCTTCTAACTTGAAAACGCATGTCAAAACTAAGCATAGTAAAGAGA TGCCATTCAAGTGTGACATTTGTCTTCTGACTTTCTCGGATACCAAAGAGGTGCAGCAACATGCTCTTAT CCACCAAGAAAGCAAAACACACCAGTGTTTGCATTGCGACCACAAGAGTTCGAACTCAAGTGATTTGAAA CGACACATAATTTCAGTTCACACGAAAGACTACCCCCATAAGTGTGACATGTGTGATAAAGGCTTTCACA GGCCTTCAGAACTCAAGAAACACGTGGCTGCCCACAAGGGCAAAAAAATGCACCAGTGTAGACATTGTGA CTTTAAGATTGCAGATCCATTTGTTCTAAGTCGCCATATTCTCTCAGTTCACACAAAGGATCTTCCATTT AGGTGCAAGAGATGTAGAAAGGGATTTAGGCAACAGAGTGAGCTTAAAAAGCATATGAAGACACACAGTG GCAGGAAAGTGTATCAGTGTGAGTACTGTGAGTATAGCACTACAGATGCCTCAGGCTTTAAACGGCACGT TATTTCCATTCACACGAAAGACTATCCTCACCGGTGTGAGTACTGCAAGAAAGGCTTCCGAAGACCTTCA GAAAAGAACCAGCACATAATGCGACATCATAAAGAAGTTGGCCTGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230300 representing NM_001178086
Red=Cloning site Green=Tags(s) MTMDTESEIDPCKVDGTCPEVIKVYIFKADPGEDDLGGTVDIVESEPENDHGVELLDQNSSIRVPREKMV YMTVNDSQPEDEDLNVAEIADEVYMEVIVGEEDAAAAAAAAAVHEQQMDDNEIKTFMPIAWAAAYGNNSD GIENRNGTASALLHIDESAGLGRLAKQKPKKRRRPDSRQYQTAIIIGPDGHPLTVYPCMICGKKFKSRGF LKRHMKNHPEHLAKKKYRCTDCDYTTNKKISLHNHLESHKLTSKAEKAIECDECGKHFSHAGALFTHKMV HKEKGANKMHKCKFCEYETAEQGLLNRHLLAVHSKNFPHICVECGKGFRHPSELKKHMRIHTGEKPYQCQ YCEYRSADSSNLKTHVKTKHSKEMPFKCDICLLTFSDTKEVQQHALIHQESKTHQCLHCDHKSSNSSDLK RHIISVHTKDYPHKCDMCDKGFHRPSELKKHVAAHKGKKMHQCRHCDFKIADPFVLSRHILSVHTKDLPF RCKRCRKGFRQQSELKKHMKTHSGRKVYQCEYCEYSTTDASGFKRHVISIHTKDYPHRCEYCKKGFRRPS EKNQHIMRHHKEVGLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001178086 |
| ORF Size | 1728 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001178086.1, NP_001171557.1 |
| RefSeq ORF | 1731 bp |
| Locus ID | 7543 |
| UniProt ID | P17010 |
| Protein Families | Transcription Factors |
| MW | 66.5 kDa |
| Gene Summary | This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230300L3 | Lenti-ORF clone of ZFX (Myc-DDK-tagged)-Human zinc finger protein, X-linked (ZFX), transcript variant 4 |
CNY 5,890.00 |
|
| RC230300L4 | Lenti-ORF clone of ZFX (mGFP-tagged)-Human zinc finger protein, X-linked (ZFX), transcript variant 4 |
CNY 5,890.00 |
|
| RG230300 | ZFX (tGFP-tagged) - Human zinc finger protein, X-linked (ZFX), transcript variant 4 |
CNY 4,370.00 |
|
| SC328938 | ZFX (untagged)-Human zinc finger protein X-linked (ZFX) transcript variant 4 |
CNY 7,220.00 |
