SCMH1 (NM_001172220) Human Tagged ORF Clone

CAT#: RC230303

SCMH1 (Myc-DDK-tagged)-Human sex comb on midleg homolog 1 (Drosophila) (SCMH1), transcript variant 5

ORF Plasmid: DDK tGFP



  "NM_001172220" in other vectors (4)

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CNY 4,296.00

CNY 5,130.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SCMH1 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms Scml3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC230303 representing NM_001172220
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAATTGGAAGCACAGGACCCCAGGAACACCACATCCACCTGTATTGCCACAGTAGTTGGACTGACAG
GTGCCCGCCTTCGCCTGCGCCTTGATGGGAGCGACAACAAAAATGACTTCTGGCGGCTGGTTGACTCAGC
TGAAATCCAGCCTATTGGGAACTGTGAAAAGAATGGGGGTATGCTACAGCCACCTCTTGGATTTCGGCTG
AATGCGTCTTCTTGGCCCATGTTCCTTTTGAAGACGCTAAATGGAGCAGAGATGGCTCCCATCAGGATTT
TCCACAAGGAGCCACCATCGCCTTCCCACAACTTCTTCAAAATGGGAATGAAGCTAGAAGCTGTGGACAG
GAAGAACCCTCATTTCATTTGCCCAGCCACTATTGGGGAGGTTCGGGGCTCAGAGGTGCTTGTCACTTTT
GATGGGTGGCGAGGGGCCTTTGACTACTGGTGCCGCTTCGACTCCCGAGACATCTTCCCTGTGGGCTGGT
GTTCCTTGACTGGAGACAACCTGCAGCCTCCTGGCACCAAAGTTGTGATTCCAAAGAATCCCTATCCTGC
CTCCGATGTGAATACTGAGAAGCCCAGCATCCACAGCAGCACCAAAACTGTCTTGGAACATCAACCAGGG
CAGAGGGGGCGTAAACCAGGAAAGAAGCGGGGCCGGACACCCAAGACCCTAATTTCCCATCCCATCTCTG
CCCCATCCAAGACAGCTGAACCTTTGAAATTCCCAAAGAAGAGAGGTCCCAAACCTGGCAGCAAGAGGAA
ACCTCGGACTTTGCTGAACCCACCACCTGCCTCACCAACAACCAGCACTCCTGAACCGGATACCAGCACT
GTACCCCAGGATGCTGCCACCATCCCCAGCTCAGCCATGCAGGCCCCAACAGTTTGTATCTACTTGAACA
AGAATGGCAGCACAGGCCCCCACTTAGATAAGAAGAAGGTCCAGCAACTCCCTGACCATTTTGGACCAGC
CCGTGCCTCTGTGGTGTTGCAGCAGGCTGTCCAGGCCTGTATCGACTGTGCTTATCACCAGAAAACCGTC
TTCAGCTTCCTCAAGCAAGGCCATGGTGGTGAGGTTATCTCAGCCGTGTTTGACCGGGAACAGCATACCC
TCAACCTCCCAGCAGTCAACAGCATCACCTACGTCCTCCGCTTCCTGGAGAAACTCTGCCACAACCTTCG
TAGTGACAATCTGTTTGGCAACCAGCCCTTTACACAGACTCACTTGTCACTCACTGCCATAGAGTACAGC
CACAGCCACGACAGGTACCTACCAGGTGAAACCTTTGTCCTGGGGAATAGTCTGGCCCGCTCCTTGGAAC
CACACTCAGACTCAATGGACTCTGCCTCAAATCCCACCAACCTTGTCAGCACCTCCCAAAGGCACCGGCC
CTTGCTTTCATCCTGTGGCCTCCCACCAAGCACTGCCTCAGCTGTGCGCAGGCTATGCTCCAGGGGGTCG
GACCGATACCTGGAGAGCCGCGATGCCTCTCGACTGAGTGGCCGGGACCCCTCCTCATGGACAGTCGAGG
ATGTGATGCAGTTTGTCCGGGAAGCTGATCCTCAGCTTGGACCCCACGCTGACCTGTTTCGCAAACACGA
GATCGATGGCAAGGCCCTGCTGCTGCTGCGCAGTGACATGATGATGAAGTACATGGGCCTGAAGCTGGGG
CCTGCACTCAAGCTCTCCTACCACATTGACCGGCTGAAGCAGGGCAAGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC230303 representing NM_001172220
Red=Cloning site Green=Tags(s)

MKLEAQDPRNTTSTCIATVVGLTGARLRLRLDGSDNKNDFWRLVDSAEIQPIGNCEKNGGMLQPPLGFRL
NASSWPMFLLKTLNGAEMAPIRIFHKEPPSPSHNFFKMGMKLEAVDRKNPHFICPATIGEVRGSEVLVTF
DGWRGAFDYWCRFDSRDIFPVGWCSLTGDNLQPPGTKVVIPKNPYPASDVNTEKPSIHSSTKTVLEHQPG
QRGRKPGKKRGRTPKTLISHPISAPSKTAEPLKFPKKRGPKPGSKRKPRTLLNPPPASPTTSTPEPDTST
VPQDAATIPSSAMQAPTVCIYLNKNGSTGPHLDKKKVQQLPDHFGPARASVVLQQAVQACIDCAYHQKTV
FSFLKQGHGGEVISAVFDREQHTLNLPAVNSITYVLRFLEKLCHNLRSDNLFGNQPFTQTHLSLTAIEYS
HSHDRYLPGETFVLGNSLARSLEPHSDSMDSASNPTNLVSTSQRHRPLLSSCGLPPSTASAVRRLCSRGS
DRYLESRDASRLSGRDPSSWTVEDVMQFVREADPQLGPHADLFRKHEIDGKALLLLRSDMMMKYMGLKLG
PALKLSYHIDRLKQGKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001172220
ORF Size 1731 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001172220.2
RefSeq ORF 1734 bp
Locus ID 22955
UniProt ID Q96GD3
Protein Families Transcription Factors
MW 64.3 kDa
Gene Summary Associates with Polycomb group (PcG) multiprotein complexes; the complex class is required to maintain the transcriptionally repressive state of some genes.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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