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BCAR1 (NM_001170720) Human Tagged ORF Clone
CAT＃: RC230457
- TrueORF®
BCAR1 (Myc-DDK-tagged)-Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 8
ORF Plasmid: tGFP
"NM_001170720" in other vectors (4)
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CNY 7,600.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAS; CAS1; CASS1; CRKAS; P130Cas |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230457 representing NM_001170720
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGGGAAGAACGTGCTGGCCAAAGCGCTCTATGACAATGTGGCCGAGTCCCCGGATGAGCTCTCCT TCCGCAAGGGTGACATCATGACGGTGCTGGAGCAGGACACGCAGGGCCTGGACGGCTGGTGGCTCTGCTC GCTGCATGGGCGCCAGGGCATCGTGCCTGGGAACCGCCTCAAGATCTTGGTGGTGGTGCCCACCCGCGTG GGGCAGGGCTATGTATACGAGGCCGCCCAGCCGGAGCAGGACGAGTACGACATCCCGCGACACCTGCTGG CCCCGGGGCCACAGGACATCTATGATGTGCCCCCGGTTCGGGGGCTGCTTCCCAGCCAGTATGGCCAGGA GGTGTATGACACACCCCCCATGGCTGTCAAGGGTCCCAATGGCCGAGACCCGTTGCTGGAGGTGTATGAC GTGCCCCCCAGTGTGGAGAAGGGCCTGCCACCGTCCAACCACCACGCAGTCTACGACGTTCCTCCATCGG TGAGCAAGGATGTGCCCGATGGCCCACTGCTGCGTGAGGAGACCTACGATGTGCCCCCCGCCTTCGCCAA GGCCAAGCCCTTTGACCCGGCCCGCACCCCACTGGTACTGGCTGCGCCCCCTCCAGACTCCCCGCCGGCC GAGGACGTGTATGACGTGCCGCCCCCGGCTCCTGACCTCTACGACGTGCCCCCTGGCTTGCGGCGGCCTG GCCCGGGCACCCTGTACGATGTGCCCCGTGAACGGGTGCTTCCTCCTGAGGTGGCTGATGGTGGCGTGGT CGACAGTGGTGTGTATGCGGTGCCTCCCCCAGCTGAACGTGAAGCCCCGGCAGAGGGCAAGCGCCTGTCG GCCTCCAGCACCGGCAGCACACGCAGCAGCCAGTCTGCGTCCTCCTTGGAGGTGGCAGGGCCGGGCCGGG AACCCCTGGAGCTGGAAGTTGCTGTGGAGGCCCTGGCACGGCTGCAGCAGGGTGTGAGCGCCACCGTTGC CCACCTTCTGGACCTGGCAGGCAGCGCCGGTGCGACTGGGAGCTGGCGTAGCCCCTCTGAGCCACAGGAG CCGCTGGTGCAGGACCTGCAGGCTGCTGTGGCCGCTGTCCAGAGTGCCGTCCACGAGCTGTTGGAGTTTG CCCGCAGCGCGGTGGGCAATGCTGCCCACACATCTGACCGTGCCCTGCATGCCAAGCTTAGCCGGCAGCT GCAGAAGATGGAGGACGTGCACCAGACGCTGGTGGCACATGGTCAGGCCCTCGACGCTGGCCGGGGAGGC TCTGGAGCCACCCTTGAGGACCTGGACCGGCTGGTGGCCTGCTCGCGGGCTGTGCCCGAGGACGCCAAGC AGCTGGCCTCCTTCCTGCACGGCAATGCCTCACTGCTCTTCAGACGGACCAAGGCCACTGCCCCGGGGCC TGAGGGGGGTGGCACCCTGCACCCCAACCCCACTGACAAGACCAGCAGCATCCAGTCACGACCCCTGCCC TCACCCCCTAAGTTCACCTCCCAGGACTCGCCAGATGGGCAGTACGAGAACAGCGAGGGGGGCTGGATGG AGGACTATGACTACGTCCACCTACAGGGGAAGGAGGAGTTTGAGAAGACCCAGAAGGAGCTGCTGGAAAA GGGCAGCATCACGCGGCAGGGCAAGAGCCAGCTGGAGTTGCAGCAGCTGAAGCAGTTTGAACGACTGGAA CAGGAGGTGTCACGGCCCATAGACCACGACCTGGCCAACTGGACGCCAGCCCAACCCCTGGCCCCGGGGC GAACAGGCGGCCTGGGGCCCTCGGACCGGCAGCTGCTGCTCTTCTACCTGGAGCAGTGTGAGGCCAACCT GACCACACTGACCAACGCCGTGGACGCCTTCTTTACCGCCGTGGCCACCAACCAGCCGCCCAAGATCTTT GTGGCGCACAGCAAGTTCGTCATCCTCAGCGCCCACAAGCTGGTGTTCATCGGGGACACACTGTCACGGC AGGCCAAGGCTGCTGACGTGCGCAGCCAGGTGACCCACTACAGCAACCTGCTGTGCGACCTCCTGCGCGG CATCGTGGCCACCACCAAGGCCGCTGCCTTGCAGTACCCATCGCCTTCCGCGGCCCAGGACATGGTGGAG AGGGTCAAGGAGCTGGGCCACAGCACCCAGCAGTTCCGCCGCGTCCTAGGCCAGCTGGCAGCCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230457 representing NM_001170720
Red=Cloning site Green=Tags(s) MQGKNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVVVPTRV GQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYD VPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPA EDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLS ASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQE PLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGG SGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLP SPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLE QEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIF VAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVE RVKELGHSTQQFRRVLGQLAAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001170720 |
| ORF Size | 2166 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001170720.3 |
| RefSeq ORF | 2169 bp |
| Locus ID | 9564 |
| UniProt ID | P56945 |
| Protein Families | Druggable Genome |
| Protein Pathways | Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton |
| MW | 78.2 kDa |
| Gene Summary | The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230457L3 | Lenti-ORF clone of BCAR1 (Myc-DDK-tagged)-Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 8 |
CNY 9,500.00 |
|
| RC230457L4 | Lenti-ORF clone of BCAR1 (mGFP-tagged)-Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 8 |
CNY 9,500.00 |
|
| RG230457 | BCAR1 (tGFP-tagged) - Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 8 |
CNY 8,360.00 |
|
| SC329095 | BCAR1 (untagged)-Human breast cancer anti-estrogen resistance 1 (BCAR1) transcript variant 8 |
CNY 13,870.00 |
