ZNF347 (NM_001172674) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ZNF1111
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC230532 ORF sequence, codon optimized. Due to the complexity of NM_001172674, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTGACCCAAGGCCAGGTGACATTTCGCGATGTTGCAATCGAGTTTTCACAAGAGGAGTGGACAT GCCTGGACCCCGCTCAGCGAACACTGTATCGCGACGTGATGCTGGAGAATTACCGCAACCTGGCCAGTCT GGCCGGGATCAGCTGTTTTGACCTGTCTATAATCAGCATGCTGGAGCAGGGCAAAGAGCCATTCACTCTG GAATCCCAGGTGCAAATCGCTGGAAACCCCGACGGCTGGGAATGGATCAAGGCGGTGATTACAGCCCTGT CATCTGAGTTTGTGATGAAGGACCTCTTGCACAAGGGCAAGAGTAATACAGGGGAAGTATTCCAGACTGT TATGCTGGAGAGGCAGGAAAGCCAAGATATCGAGGGATGTAGCTTCAGGGAGGTTCAGAAAAATACACAT GGTCTGGAGTATCAGTGCCGCGATGCTGAAGGTAATTACAAAGGCGTATTGCTGACCCAGGAGGGCAACC TGACCCACGGGCGCGATGAGCACGATAAGCGAGATGCCAGAAATAAACTTATCAAGAACCAGCTCGGCCT GTCTCTTCAGTCACACCTGCCAGAACTGCAGCTCTTTCAATACGAAGGTAAAATTTACGAATGCAATCAG GTCGAGAAGAGTTTCAATAATAACTCCAGCGTGAGCCCCCCTCAACAAATGCCTTATAACGTAAAAACCC ACATTTCCAAGAAGTATCTCAAGGATTTCATCTCTAGTCTCCTGCTCACCCAAGGCCAAAAGGCAAACAA TTGGGGAAGTCCTTACAAAAGCAACGGGTGTGGAATGGTCTTCCCTCAAAATTCTCATCTGGCATCTCAC CAGAGGAGTCACACCAAAGAAAAGCCCTACAAATGCTATGAATGCGGGAAGGCTTTCCGAACACGGAGCA ACCTGACAACCCACCAAGTGATCCACACTGGCGAGAAAAGATATAAATGCAATGAGTGCGGAAAGGTGTT CTCAAGGAATTCCCAGCTGTCTCAACATCAGAAAATCCACACTGGGGAAAAACCTTACAAGTGCAATGAG TGTGGTAAGGTGTTCACCCAGAACTCACACTTGGTTCGCCATAGGGGTATTCACACTGGGGAGAAGCCGT ACAAGTGCAATGAATGTGGGAAAGCCTTCCGCGCCCGGTCCTCTCTGGCGATACACCAGGCTACACACTC TGGCGAAAAACCCTATAAGTGCAATGAGTGTGGAAAAGTCTTTACTCAGAACTCCCACTTGACTAACCAC TGGCGCATCCACACCGGCGAGAAACCATACAAGTGCAATGAATGCGGCAAGGCATTCGGTGTGCGGAGTA GCCTGGCTATCCACTTGGTGATCCATACGGGTGAAAAGCCATATAAATGCCACGAGTGTGGTAAGGTCTT CCGCCGGAATTCCCACCTCGCTCGACATCAGCTGATTCACACAGGGGAGAAGCCCTATAAGTGCAACGAG TGCGGCAAAGCCTTCCGCGCTCACTCCAATCTGACAACTCACCAAGTTATACACACCGGGGAGAAGCCTT ATAAGTGTAATGAATGTGGCAAAGTCTTCACCCAGAATTCACACCTGGCTAACCACCAGCGGATCCACAC AGGTGTAAAACCATACATGTGCAATGAGTGCGGTAAGGCCTTCAGTGTTTACTCCTCTCTGACCACTCAC CAGGTGATCCATACAGGGGAGAAACCTTACAAATGCAACGAGTGTGGCAAAGTTTTTACTCAAAATTCAC ACCTGGCTCGGCACCGAGGCATTCACACAGGCGAGAAACCGTACAAGTGTAATGAGTGTGGGAAGGTATT CAGGCATAACAGCTACCTTTCCCGCCACCAACGGATCCACACTGGTGAGAAGCCATATAAATACAACGAA TACGGGAAGGCGTTCAGCGAGCACTCTAACCTGACGACCCACCAGGTTATTCACACTGGCGAGAAGCCCT ATAAGTGTAACGAGTGTGGGAAAGTTTTCACCCAAAACTCACACCTGGCAAGACACCGCCGAGTTCATAC AGGGGGGAAACCATATCAGTGTAACGAATGTGGCAAAGCGTTTTCTCAGACTTCAAAATTGGCGAGGCAC CAAAGAGTCCATACAGGGGAGAAGCCATACGAGTGCAACCAATGCGGGAAGGCCTTCTCCGTGAGATCTA GCCTCACAACACATCAGGCTATTCACACCGGTAAGAAACCCTACAAGTGTAACGAGTGTGGCAAAGTTTT TACTCAAAACAGCCATCTGGCAAGGCACCGCGGGATACACACCGGCGAGAAGCCATACAAGTGCAATGAA TGCGGGAAGGCCTTCTCCCAAACCTCCAAATTGGCGAGACATCAGAGAATCCATACAGGGGAAAAACCAT ACGAATGCGGTAAGCCTTTCTCAATTTGCAGTTCCCTGACCACCCATCAGACCATACACACCGGAGGAAA GCCCTACAAGTGTAACGTGTGGAAGGTGCTGAAATCTGAGTTCAAGCCCTGTAAGCCCTCACAAAATAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC230532 representing NM_001172674 Red=Cloning site Green=Tags(s) MALTQGQVTFRDVAIEFSQEEWTCLDPAQRTLYRDVMLENYRNLASLAGISCFDLSIISMLEQGKEPFTL ESQVQIAGNPDGWEWIKAVITALSSEFVMKDLLHKGKSNTGEVFQTVMLERQESQDIEGCSFREVQKNTH GLEYQCRDAEGNYKGVLLTQEGNLTHGRDEHDKRDARNKLIKNQLGLSLQSHLPELQLFQYEGKIYECNQ VEKSFNNNSSVSPPQQMPYNVKTHISKKYLKDFISSLLLTQGQKANNWGSPYKSNGCGMVFPQNSHLASH QRSHTKEKPYKCYECGKAFRTRSNLTTHQVIHTGEKRYKCNECGKVFSRNSQLSQHQKIHTGEKPYKCNE CGKVFTQNSHLVRHRGIHTGEKPYKCNECGKAFRARSSLAIHQATHSGEKPYKCNECGKVFTQNSHLTNH WRIHTGEKPYKCNECGKAFGVRSSLAIHLVIHTGEKPYKCHECGKVFRRNSHLARHQLIHTGEKPYKCNE CGKAFRAHSNLTTHQVIHTGEKPYKCNECGKVFTQNSHLANHQRIHTGVKPYMCNECGKAFSVYSSLTTH QVIHTGEKPYKCNECGKVFTQNSHLARHRGIHTGEKPYKCNECGKVFRHNSYLSRHQRIHTGEKPYKYNE YGKAFSEHSNLTTHQVIHTGEKPYKCNECGKVFTQNSHLARHRRVHTGGKPYQCNECGKAFSQTSKLARH QRVHTGEKPYECNQCGKAFSVRSSLTTHQAIHTGKKPYKCNECGKVFTQNSHLARHRGIHTGEKPYKCNE CGKAFSQTSKLARHQRIHTGEKPYECGKPFSICSSLTTHQTIHTGGKPYKCNVWKVLKSEFKPCKPSQNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001172674
ORF Size	2520 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001172674.1, NP_001166145.1
RefSeq Size	4565 bp
RefSeq ORF	2523 bp
Locus ID	84671
UniProt ID	Q96SE7
Protein Families	Transcription Factors
MW	95.8 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]

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