United States
Germany
Japan
United Kingdom
China
AFF2 (NM_001169125) Human Tagged ORF Clone
CAT＃: RC230688
- TrueORF®
AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 5
ORF Plasmid: tGFP
"NM_001169125" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 12,730.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230688 representing NM_001169125
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTATTCGACTTTTTCAGAGACTGGGACTTGGAGCAGCAGTGTCACTATGAACAAGACCGTAGTG CACTTAAAAAAAGGGAATGGGAGCGGAGGAATCAAGAAGTCCAGCAAGAAGACGATCTCTTTTCTTCAGG CTTTGATCTTTTTGGGGAGCCATACAAGACAAACAAAGGTGATGCACTTGCCAACCGAGTCCAGAACACG CTTGGAAACTATGATGAAATGAAGAATTTGCTAACTAACCATTCTAATCAGAATCACCTAGTGGGAATTC CAAAGAATTCTGTGCCCCAGAATCCCAACAACAAAAATGAACCAAGCTTTTTTCCAGAACAAAAGAACAG AATAATTCCACCTCACCAGGATAATACCCATCCTTCAGCACCAATGCCTCCACCTTCTGTTGTGATACTG AATTCAACTCTAATACACAGCAACAGAAAATCAAAACCTGAGTGGTCACGTGATAGTCATAACCCTAGCA CTGTACTGGCAAGCCAGGCCAGTGGTCAGCCAAACAAGATGCAGACTTTGACACAGGACCAGTCTCAAGC CAAACTGGAAGACTTCTTTGTCTACCCAGCTGAACAGCCCCAGATTGGAGAAGTTGAAGAGTCAAACCCA TCTGCAAAGGAAGACAGTAACCCTAATTCTAGTGGAGAAGATGCTTTCAAAGAAATCTTTCAATCCAATT CACCGGAAGAATCTGAATTCGCCGTGCAAGCGCCTGGGTCTCCCCTAGTGGCTTCCTCTTTATTAGCTCC TAGCAGTGGCCTTTCAGTTCAAAACTTCCCACCAGGGCTTTACTGCAAAACAAGCATGGGGCAGCAAAAG CCAACTGCATACGTCAGACCCATGGATGGCCAGGACCAGGCACCGGACATCTCACCAACACTGAAACCTT CAATTGAATTTGAGAACAGCTTTGGGAATCTGTCATTTGGAACACTCTTGGATGGAAAACCCAGTGCAGC CAGTTCAAAGACTAAACTGCCAAAGTTCACCATCCTCCAAACAAGTGAAGTAAGCCTTCCCAGTGATCCA AGCTGTGTTGAAGAAATCTTGCGGGAATCGCAGCATCTGACCCCAGGATTCACCTTACAAAAGTGGAATG ACCCAACCACCAGAGCTTCTACAAAGATGCTTGAGGATGACCTGAAGCTGAGCAGTGATGAAGATGACCT TGAGCCTGTGAAGACCTTGACCACTCAGTGCACTGCCACTGAGCTCTACCAGGCTGTTGAAAAGGCAAAA CCTAGGAATAATCCTGTGAACCCACCCTTGGCCACTCCCCAGCCCCCACCTGCAGTGCAAGCCAGCGGGG GTTCTGGCAGCTCCAGCGAATCGGAGAGCAGCTCTGAGTCGGATTCAGACACTGAAAGTAGCACCACTGA CAGCGAATCTAATGAGGCACCTCGTGTGGCAACTCCAGAGCCTGAGCCACCCTCAACCAACAAGTGGCAA CTGGATAAATGGCTTAACAAAGTGACATCCCAGAACAAGTCTTTTATTTGTGGCCAAAATGAAACACCCA TGGAGACTATTTCTCTGCCTCCTCCAATCATCCAACCAATGGAAGTCCAGATGAAAGTGAAGACGAATGC CAGTCAGGTCCCAGCTGAACCCAAAGAAAGGCCTCTCCTCAGTCTCATTAGGGAGAAAGCCCGTCCACGG CCCACTCAGAAAATTCCAGAAACAAAGGCTTTGAAGCATAAGTTGTCAACAACTAGTGAGACAGTGTCTC AAAGGACAATTGGGAAAAAACAGCCCAAAAAAGTTGAGAAGAACACCAGCACTGACGAGTTTACCTGGCC CAAACCAAATATTACCAGCAGCACTCCCAAAGAAAAAGAAAGTGTGGAGCTTCATGACCCACCAAGAGGC CGCAACAAAGCCACTGCCCACAAACCAGCCCCTAGGAAAGAACCAAGACCTAACATCCCTTTGGCTCCCG AGAAGAAGAAGTACAGAGGGCCTGGCAAGATTGTGCCAAAGTCTCGGGAATTCATTGAAACAGATTCATC TACATCTGACTCCAACACAGATCAGGAAGAGACCCTGCAAATCAAAGTCCTGCCTCCGTGCATTATTTCT GGAGGTAATACTGCCAAATCCAAGGAAATCTGTGGTGCCAGCCTGACCCTCAGCACCTTAATGAGTAGCA GTGGCAGCAACAACAACTTATCCATCAGTAATGAAGAGCCAACATTTTCACCTATTCCTGTCATGCAAAC TGAAATCCTGTCCCCTCTGCGAGATCATGAGAACCTGAAAAACCTCTGGGTGAAGATTGACCTTGACTTA CTCTCTAGAGTACCTGGCCACAGCTCACTCCATGCAGCACCTGCCAAGCCAGACCACAAGGAGACTGCCA CAAAACCCAAGCGTCAGACAGCTGTCACAGCTGTGGAGAAACCAGCCCCTAAGGGCAAACGTAAGCACAA GCCAATAGAAGTTGCAGAGAAGATCCCTGAGAAGAAGCAGCGCCTGGAGGAGGCCACAACTATCTGCTTG CTCCCTCCTTGCATCTCACCAGCCCCACCCCACAAGCCTCCCAACACTAGAGAAAATAATTCATCCAGGA GAGCAAATAGAAGAAAGGAAGAAAAACTATTTCCTCCTCCACTTTCCCCACTGCCAGAGGACCCTCCACG CCGCAGAAATGTCAGTGGCAATAATGGTCCCTTTGGTCAAGACAAAAACATCGCCATGACTGGACAAATC ACATCTACCAAACCTAAGAGAACTGAAGGCAAATTCTGTGCTACTTTCAAAGGGATATCGGTAAATGAGG GAGACACTCCAAAAAAGGCATCCTCTGCCACCATCACTGTCACCAATACTGCTATTGCCACTGCTACTGT CACTGCTACTGCCATTGTCACCACCACTGTCACAGCTACTGCCACCGCCACGGCCACCACCACAACTACT ACCACTACCATTTCCACCATCACCTCTACCATCACTACTGGCCTCATGGATAGCAGTCACCTGGAGATGA CGTCCTGGGCGGCTCTGCCCCTTCTATCCAGCAGCAGCACTAATGTCCGGAGACCCAAGCTCACTTTTGA TGACTCGGTTCACAATGCTGATTATTACATGCAAGAAGCTAAGAAGCTGAAGCACAAAGCTGATGCACTG TTCGAGAAATTTGGCAAAGCTGTGAATTATGCTGATGCCGCCCTCTCCTTCACTGAATGTGGCAATGCCA TGGAACGCGACCCTCTGGAAGCAAAGTCCCCATACACCATGTACTCTGAGACTGTGGAGCTCCTCAGGTA TGCAATGAGGCTGAAGAACTTTGCAAGTCCCTTGGCTTCGGATGGGGACAAAAAGCTAGCAGTACTATGC TACCGATGTTTATCACTCCTCTATTTGAGAATGTTTAAGCTGAAGAAGGACCATGCTATGAAGTACTCCA GATCACTGATGGAATATTTTAAGCAAAATGCTTCAAAAGTCGCACAGATACCCTCTCCATGGGTAAGCAA TGGAAAGAACACTCCATCCCCAGTGTCTCTCAACAACGTCTCCCCCATCAACGCAATGGGGAACTGTAAC AATGGCCCAGTCACCATTCCCCAGCGCATTCACCACATGGCTGCCAGCCACGTCAACATCACTAGCAATG TGTTACGGGGCTATGAACACTGGGATATGGCCGACAAACTGACAAGAGAAAACAAAGAATTCTTTGGTGA TCTGGACACGCTGATGGGGCCTCTGACCCAGCACAGCAGCATGACCAATCTTGTCCGCTACGTTCGCCAA GGACTGTGTTGGCTGCGCATCGATGCCCACTTGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230688 representing NM_001169125
Red=Cloning site Green=Tags(s) MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLFSSGFDLFGEPYKTNKGDALANRVQNT LGNYDEMKNLLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVIL NSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNP SAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYCKTSMGQQK PTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSKTKLPKFTILQTSEVSLPSDP SCVEEILRESQHLTPGFTLQKWNDPTTRASTKMLEDDLKLSSDEDDLEPVKTLTTQCTATELYQAVEKAK PRNNPVNPPLATPQPPPAVQASGGSGSSSESESSSESDSDTESSTTDSESNEAPRVATPEPEPPSTNKWQ LDKWLNKVTSQNKSFICGQNETPMETISLPPPIIQPMEVQMKVKTNASQVPAEPKERPLLSLIREKARPR PTQKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTSTDEFTWPKPNITSSTPKEKESVELHDPPRG RNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSREFIETDSSTSDSNTDQEETLQIKVLPPCIIS GGNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEEPTFSPIPVMQTEILSPLRDHENLKNLWVKIDLDL LSRVPGHSSLHAAPAKPDHKETATKPKRQTAVTAVEKPAPKGKRKHKPIEVAEKIPEKKQRLEEATTICL LPPCISPAPPHKPPNTRENNSSRRANRRKEEKLFPPPLSPLPEDPPRRRNVSGNNGPFGQDKNIAMTGQI TSTKPKRTEGKFCATFKGISVNEGDTPKKASSATITVTNTAIATATVTATAIVTTTVTATATATATTTTT TTTISTITSTITTGLMDSSHLEMTSWAALPLLSSSSTNVRRPKLTFDDSVHNADYYMQEAKKLKHKADAL FEKFGKAVNYADAALSFTECGNAMERDPLEAKSPYTMYSETVELLRYAMRLKNFASPLASDGDKKLAVLC YRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKQNASKVAQIPSPWVSNGKNTPSPVSLNNVSPINAMGNCN NGPVTIPQRIHHMAASHVNITSNVLRGYEHWDMADKLTRENKEFFGDLDTLMGPLTQHSSMTNLVRYVRQ GLCWLRIDAHLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001169125 |
| ORF Size | 3816 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001169125.2 |
| RefSeq ORF | 3819 bp |
| Locus ID | 2334 |
| UniProt ID | P51816 |
| Protein Families | Druggable Genome |
| MW | 141 kDa |
| Gene Summary | This gene encodes a putative transcriptional activator that is a member of the AF4\FMR2 gene family. This gene is associated with the folate-sensitive fragile X E locus on chromosome X. A repeat polymorphism in the fragile X E locus results in silencing of this gene causing Fragile X E syndrome. Fragile X E syndrome is a form of nonsyndromic X-linked cognitive disability. In addition, this gene contains 6-25 GCC repeats that are expanded to >200 repeats in the disease state. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230688L3 | Lenti-ORF clone of AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 5 |
CNY 14,630.00 |
|
| RC230688L4 | Lenti-ORF clone of AFF2 (mGFP-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 5 |
CNY 14,630.00 |
|
| RG230688 | AFF2 (tGFP-tagged) - Human AF4/FMR2 family, member 2 (AFF2), transcript variant 5 |
CNY 13,970.00 |
|
| SC329326 | AFF2 (untagged)-Human AF4/FMR2 family member 2 (AFF2) transcript variant 5 |
CNY 10,450.00 |
