NrCAM (NM_001193584) Human Tagged ORF Clone
CAT#: RC230893
- TrueORF®
NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 6
ORF Plasmid: tGFP
"NM_001193584" in other vectors (3)
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CNY 11,780.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC230893 representing NM_001193584
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCTTAAAATAATGCCGAAAAAGAAGCGCTTATCTGCGGGCAGAGTGCCCCTGATTCTCTTCCTGT GCCAGATGATTAGTGCACTGGAAGTACCTCTTGATCCAAAACTTCTTGAAGACTTGGTACAGCCTCCAAC CATCACCCAACAGTCTCCAAAAGATTACATTATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAAGCC AAAGGGAAACCGCCCCCAAGCTTTTCCTGGACCCGTAATGGGACTCATTTTGACATCGATAAAGACCCTC TGGTCACCATGAAGCCTGGCACAGGAACGCTCATAATTAACATCATGAGCGAAGGGAAAGCTGAGACCTA TGAAGGAGTCTATCAGTGTACAGCAAGGAACGAACGCGGAGCTGCAGTTTCTAATAACATTGTTGTCCGC CCATCCAGATCACCATTGTGGACCAAAGAAAAACTTGAACCAATCACACTTCAAAGTGGTCAGTCTTTAG TACTTCCCTGCAGACCCCCAATTGGATTACCACCACCTATAATATTTTGGATGGATAATTCCTTTCAAAG ACTTCCACAAAGTGAGAGAGTTTCTCAAGGTTTGAATGGGGACCTTTATTTTTCCAATGTCCTCCCAGAG GACACCCGCGAAGACTATATCTGTTATGCTAGATTTAATCATACTCAAACCATACAGCAGAAGCAACCTA TTTCTGTGAAGGTGATTTCAGCTAAATCAAGTAGAGAGAGGCCACCAACATTTTTAACTCCAGAAGGCAA TGCAAGTAACAAAGAGGAATTAAGAGGAAATGTGCTTTCACTGGAGTGCATTGCAGAAGGACTGCCTACC CCAATTATTTACTGGGCAAAGGAAGATGGAATGCTACCCAAAAACAGGACAGTTTATAAGAACTTTGAGA AAACCTTGCAGATCATTCATGTTTCAGAAGCAGACTCTGGAAATTACCAATGTATAGCAAAAAATGCATT AGGAGCCATCCACCATACCATTTCTGTTAGAGTTAAAGCGGCTCCATACTGGATCACAGCCCCTCAAAAT CTTGTGCTGTCCCCAGGAGAGGATGGGACCTTGATCTGCAGAGCTAATGGCAACCCCAAACCCAGAATTA GCTGGTTAACAAATGGAGTCCCAATAGAAATTGCCCCTGATGACCCCAGCAGAAAAATAGATGGCGATAC CATTATTTTTTCAAATGTTCAAGAAAGATCAAGTGCAGTATATCAGTGCAATGCCTCTAATGAATATGGA TATTTACTGGCAAACGCATTTGTAAATGTGCTGGCTGAGCCACCACGAATCCTCACACCTGCAAACACAC TCTACCAGGTCATTGCAAACAGGCCTGCTTTACTAGACTGTGCCTTCTTTGGGTCTCCTCTCCCAACCAT CGAGTGGTTTAAAGGAGCTAAAGGAAGTGCTCTTCATGAAGATATTTATGTTTTACATGAAAATGGAACT TTGGAAATTCCTGTGGCCCAAAAGGACAGTACAGGAACTTATACGTGTGTTGCAAGGAATAAATTAGGGA TGGCAAAGAATGAAGTTCACTTAGAAATCAAAGATGCTACATGGATCGTTAAACAGCCCGAATATGCAGT TGTGCAAAGAGGGAGCATGGTGTCCTTTGAATGCAAAGTGAAACATGATCACACCTTATCCCTCACTGTC CTGTGGCTGAAGGACAACAGGGAACTGCCCAGTGATGAAAGGTTCACTGTTGACAAGGATCATCTAGTGG TAGCTGATGTCAGTGACGATGACAGCGGGACCTACACGTGTGTGGCCAACACCACTCTGGACAGCGTCTC CGCCAGCGCTGTGCTTAGCGTTGTTGCTCCTACTCCAACTCCAGCTCCCGTTTACGATGTCCCAAATCCT CCCTTTGACTTAGAACTGACAGATCAACTTGACAAAAGTGTTCAGCTGTCATGGACCCCAGGCGATGACA ACAATAGCCCCATTACAAAATTCATCATCGAATATGAAGATGCAATGCACAAGCCAGGGCTGTGGCACCA CCAAACTGAAGTTTCTGGAACACAGACCACAGCCCAGCTGAAGCTGTCTCCTTACGTGAACTACTCCTTC CGCGTGATGGCAGTGAACAGCATTGGGAAGAGCTTGCCCAGCGAGGCCTCTGAGCAGTATTTGACGAAAG CCTCAGAACCAGATAAAAACCCCACAGCTGTGGAAGGACTGGGATCAGAGCCTGATAATTTGGTGATTAC GTGGAAGCCCTTGAATGGTTTCGAATCTAATGGGCCAGGCCTTCAGTACAAAGTTAGCTGGCGCCAGAAA GATGGTGATGATGAATGGACATCTGTGGTTGTGGCAAATGTATCCAAATATATTGTCTCAGGCACGCCAA CCTTTGTTCCATACCTGATCAAAGTTCAGGCCCTGAATGACATGGGGTTTGCCCCCGAGCCAGCTGTAGT CATGGGACATTCTGGAGAAGACCTCCCAATGGTGGCTCCTGGGAACGTGCGTGTGAATGTGGTGAACAGT ACCTTAGCCGAGGTGCACTGGGACCCAGTACCTCTGAAAAGCATCCGAGGACACCTACAAGGCTATCGGA TTTACTATTGGAAGACCCAGAGTTCATCTAAAAGAAACAGACGTCACATTGAGAAAAAGATCCTCACCTT CCAAGGCAGCAAGACTCATGGCATGTTGCCGGGGCTAGAGCCCTTTAGCCACTACACACTGAATGTCCGA GTGGTCAATGGGAAAGGGGAGGGCCCAGCCAGCCCTGACAGAGTCTTTAATACTCCAGAAGGAGTCCCCA GTGCTCCCTCGTCTTTGAAGATTGTGAATCCAACACTGGACTCTCTCACTTTGGAATGGGATCCACCGAG CCACCCGAATGGCATTTTGACAGAGTACACCTTAAAGTATCAGCCAATTAACAGCACACATGAATTAGGC CCTCTGGTAGATTTGAAAATTCCTGCCAACAAGACACGGTGGACTTTAAAAAATTTAAATTTCAGCACTC GATATAAGTTTTATTTCTATGCACAAACATCAGCAGGATCAGGAAGTCAAATTACAGAGGAAGCAGTAAC AACTGTGGATGAAGCGATGGCAAGCCGGCAGGTGGATATTGCAACTCAGGGCTGGTTCATTGGTCTGATG TGTGCTGTTGCTCTCCTTATCTTAATTTTGCTGATTGTTTGCTTCATCAGAAGAAACAAGGGTGGTAAAT ATCCAGTTAAAGAAAAGGAAGATGCCCATGCTGACCCTGAAATCCAGCCTATGAAGGAAGATGATGGGAC ATTTGGAGAATACAGTGATGCAGAAGACCACAAGCCTTTGAAAAAAGGAAGTCGAACTCCTTCAGACAGG ACTGTGAAAAAAGAAGATAGTGACGACAGCCTAGTTGACTATGGAGAAGGGGTTAATGGCCAGTTCAATG AGGATGGCTCCTTTATTGGACAATACAGTGGTAAGAAAGAGAAAGAGCCGGCTGAAGGAAACGAAAGCTC AGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230893 representing NM_001193584
Red=Cloning site Green=Tags(s) MQLKIMPKKKRLSAGRVPLILFLCQMISALEVPLDPKLLEDLVQPPTITQQSPKDYIIDPRENIVIQCEA KGKPPPSFSWTRNGTHFDIDKDPLVTMKPGTGTLIINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVR PSRSPLWTKEKLEPITLQSGQSLVLPCRPPIGLPPPIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPE DTREDYICYARFNHTQTIQQKQPISVKVISAKSSRERPPTFLTPEGNASNKEELRGNVLSLECIAEGLPT PIIYWAKEDGMLPKNRTVYKNFEKTLQIIHVSEADSGNYQCIAKNALGAIHHTISVRVKAAPYWITAPQN LVLSPGEDGTLICRANGNPKPRISWLTNGVPIEIAPDDPSRKIDGDTIIFSNVQERSSAVYQCNASNEYG YLLANAFVNVLAEPPRILTPANTLYQVIANRPALLDCAFFGSPLPTIEWFKGAKGSALHEDIYVLHENGT LEIPVAQKDSTGTYTCVARNKLGMAKNEVHLEIKDATWIVKQPEYAVVQRGSMVSFECKVKHDHTLSLTV LWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVANTTLDSVSASAVLSVVAPTPTPAPVYDVPNP PFDLELTDQLDKSVQLSWTPGDDNNSPITKFIIEYEDAMHKPGLWHHQTEVSGTQTTAQLKLSPYVNYSF RVMAVNSIGKSLPSEASEQYLTKASEPDKNPTAVEGLGSEPDNLVITWKPLNGFESNGPGLQYKVSWRQK DGDDEWTSVVVANVSKYIVSGTPTFVPYLIKVQALNDMGFAPEPAVVMGHSGEDLPMVAPGNVRVNVVNS TLAEVHWDPVPLKSIRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGSKTHGMLPGLEPFSHYTLNVR VVNGKGEGPASPDRVFNTPEGVPSAPSSLKIVNPTLDSLTLEWDPPSHPNGILTEYTLKYQPINSTHELG PLVDLKIPANKTRWTLKNLNFSTRYKFYFYAQTSAGSGSQITEEAVTTVDEAMASRQVDIATQGWFIGLM CAVALLILILLIVCFIRRNKGGKYPVKEKEDAHADPEIQPMKEDDGTFGEYSDAEDHKPLKKGSRTPSDR TVKKEDSDDSLVDYGEGVNGQFNEDGSFIGQYSGKKEKEPAEGNESSEAPSPVNAMNSFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001193584 |
ORF Size | 3540 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001193584.1, NP_001180513.1 |
RefSeq ORF | 3543 bp |
Locus ID | 4897 |
UniProt ID | Q92823 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Cell adhesion molecules (CAMs) |
MW | 131.1 kDa |
Gene Summary | Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230893L3 | Lenti-ORF clone of NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 6 |
CNY 13,680.00 |
|
RC230893L4 | Lenti-ORF clone of NRCAM (mGFP-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 6 |
CNY 13,680.00 |
|
RG230893 | NRCAM (tGFP-tagged) - Human neuronal cell adhesion molecule (NRCAM), transcript variant 6 |
CNY 12,920.00 |