CNOT4 (NM_001190848) Human Tagged ORF Clone

CAT#: RC231320

CNOT4 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 4

ORF Plasmid: DDK tGFP



  "NM_001190848" in other vectors (3)

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CNY 4,280.00

CNY 5,040.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CNOT4 mouse monoclonal antibody, clone OTI1H2 (formerly 1H2)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CLONE243; NOT4; NOT4H
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC231320 representing NM_001190848
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCGCAGTCCTGATGCGAAGGAAGACCCTGTGGAGTGCCCTCTTTGCATGGAGCCCTTGGAGATAG
ATGATATCAACTTTTTCCCTTGCACCTGTGGCTACCAGATTTGCCGATTTTGTTGGCATCGAATTCGCAC
TGATGAAAATGGGCTTTGTCCTGCATGTAGAAAGCCATATCCAGAAGACCCAGCAGTTTATAAACCACTC
TCCCAGGAAGAGCTGCAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAGAGAAAACAGAAAATAT
CAGAAAATCGCAAACATTTGGCTAGTGTACGTGTCGTACAAAAAAACCTCGTCTTTGTTGTAGGTTTATC
TCAGCGCCTAGCAGACCCAGAGGTTTTAAAACGACCAGAATATTTTGGGAAGTTTGGTAAAATACATAAA
GTTGTCATCAATAATAGCACATCATATGCAGGCTCACAGGGTCCAAGTGCCAGTGCTTATGTAACCTATA
TCCGGTCAGAAGACGCTCTCAGAGCCATACAGTGTGTCAACAATGTGGTAGTAGATGGCAGAACACTTAA
GGCATCTCTAGGTACAACAAAATACTGCAGTTACTTCTTAAAGAATATGCAGTGTCCAAAACCTGACTGC
ATGTATCTTCATGAATTGGGGGATGAGGCGGCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAACACC
AAGAATATGAACAGAAGCTACTTCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGG
TTCAGTTGATAAAAATAAGAACAAAGTGACACCACTGCAGAGGTACGATACCCCCATTGACAAACCTTCA
GATTCTCTCAGTATAGGGAACGGTGATAATTCCCAGCAGATATCTAACAGTGATACGCCTTCACCACCAC
CTGGTTTGTCAAAATCCAATCCAGTCATCCCCATCAGTTCATCCAATCACAGTGCACGGTCCCCTTTTGA
AGGGGCAGTAACAGAGTCACAGTCGTTATTCTCAGACAATTTTCGCCATCCCAACCCTATCCCAAGTGGG
CTTCCTCCTTTCCCCAGCTCCCCACAGACATCCAGTGACTGGCCTACAGCACCAGAACCACAGAGCCTCT
TCACATCAGAAACAATCCCAGTATCATCCTCTACAGACTGGCAAGCAGCTTTTGGCTTTGGTTCTTCTAA
ACAACCAGAGGATGACTTGGGTTTTGATCCCTTCGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAG
AAGGAACTGTCCGTTCAAGACCAACCTTCCCTTTCGCCCACATCTCTTCAGAACTCCTCTTCACACACTA
CAACCGCCAAAGGTCCAGGCTCTGGATTCCTGCATCCTGCTGCAGCTACAAATGCCAATTCTCTCAATAG
TACCTTTTCAGTCTTGCCCCAGAGGTTCCCTCAATTTCAGCAGCACCGAGCGGTTTATAATTCATTCAGT
TTTCCAGGCCAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACA
CAGCAAACCCCACCTCAAATAGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGG
AGGGATCCCTGTAGCAGGGGAAGAAGAGGTGAAGGTTTCGACCATGCCACTGTCAACCTCTTCCCATTCA
TTACAACAAGGACAGCAGCCTACAAGTCTCCACACTACTGTGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC231320 representing NM_001190848
Red=Cloning site Green=Tags(s)

MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPL
SQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHK
VVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDC
MYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQRYDTPIDKPS
DSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSG
LPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIE
KELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFS
FPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVAGEEEVKVSTMPLSTSSHS
LQQGQQPTSLHTTVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001190848
ORF Size 1725 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001190848.1, NP_001177777.1
RefSeq ORF 1728 bp
Locus ID 4850
UniProt ID O95628
Protein Families Druggable Genome, Transcription Factors
Protein Pathways RNA degradation
MW 64 kDa
Gene Summary The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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