A1CF (NM_001198819) Human Tagged Lenti ORF Clone
CAT#: RC231331L3
- LentiORF®
Lenti-ORF clone of A1CF (Myc-DDK-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 5
"NM_001198819" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 6,460.00
Product images
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ACF; ACF64; ACF65; APOBEC1CF; ASP |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC231331).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001198819 |
ORF Size | 1806 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001198819.1, NP_001185748.1 |
RefSeq ORF | 1809 bp |
Locus ID | 29974 |
UniProt ID | Q9NQ94 |
MW | 66.4 kDa |
Gene Summary | Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231331 | A1CF (Myc-DDK-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 5 |
CNY 4,480.00 |
|
RC231331L4 | Lenti-ORF clone of A1CF (mGFP-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 5 |
CNY 6,460.00 |
|
RG231331 | A1CF (tGFP-tagged) - Human APOBEC1 complementation factor (A1CF), transcript variant 5 |
CNY 5,040.00 |