PDE4D (NM_001197220) Human Tagged ORF Clone
CAT#: RC231369
PDE4D (Myc-DDK-tagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 6
ORF Plasmid: tGFP
"NM_001197220" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 7,456.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ACRDYS2; DPDE3; HSPDE4D; PDE4DN2; PDE43; STRK1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC231369 representing NM_001197220
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATTATTATGAAGCCAAGATCCCGATCTACAAGTTCCCTAAGGACTGCAGAGGCAGTTTGTTTTG ATGTGGACAATGGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGATCCGGGCTAAT TCTCCAAGCAAATTTTGTCCACAGTCAACGACGGGAGTCCTTCCTGTATCGATCCGACAGCGATTATGAC CTCTCTCCAAAGTCTATGTCCCGGAACTCCTCCATTGCCAGTGATATACACGGAGATGACTTGATTGTGA CTCCATTTGCTCAGGTCTTGGCCAGTCTGCGAACTGTACGAAACAACTTTGCTGCATTAACTAATTTGCA AGATCGAGCACCTAGCAAAAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATAACAGAG GAGGCCTACCAGAAACTGGCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCC TACAGACCAGGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTTAAAAGGATGCTTAATCGGGAGCTCAC CCATCTCTCTGAAATGAGTCGGTCTGGAAATCAAGTGTCAGAGTTTATATCAAACACATTCTTAGATAAG CAACATGAAGTGGAAATTCCTTCTCCAACTCAGAAGGAAAAGGAGAAAAAGAAAAGACCAATGTCTCAGA TCAGTGGAGTCAAGAAATTGATGCACAGCTCTAGTCTGACTAATTCAAGTATCCCAAGGTTTGGAGTTAA AACTGAACAAGAAGATGTCCTTGCCAAGGAACTAGAAGATGTGAACAAATGGGGTCTTCATGTTTTCAGA ATAGCAGAGTTGTCTGGTAACCGGCCCTTGACTGTTATCATGCACACCATTTTTCAGGAACGGGATTTAT TAAAAACATTTAAAATTCCAGTAGATACTTTAATTACATATCTTATGACTCTCGAAGACCATTACCATGC TGATGTGGCCTATCACAACAATATCCATGCTGCAGATGTTGTCCAGTCTACTCATGTGCTATTATCTACA CCTGCTTTGGAGGCTGTGTTTACAGATTTGGAGATTCTTGCAGCAATTTTTGCCAGTGCAATACATGATG TAGATCATCCTGGTGTGTCCAATCAATTTCTGATCAATACAAACTCTGAACTTGCCTTGATGTACAATGA TTCCTCAGTCTTAGAGAACCATCATTTGGCTGTGGGCTTTAAATTGCTTCAGGAAGAAAACTGTGACATT TTCCAGAATTTGACCAAAAAACAAAGACAATCTTTAAGGAAAATGGTCATTGACATCGTACTTGCAACAG ATATGTCAAAACACATGAATCTACTGGCTGATTTGAAGACTATGGTTGAAACTAAGAAAGTGACAAGCTC TGGAGTTCTTCTTCTTGATAATTATTCCGATAGGATTCAGGTTCTTCAGAATATGGTGCACTGTGCAGAT CTGAGCAACCCAACAAAGCCTCTCCAGCTGTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCC GCCAAGGAGACCGAGAGAGGGAACGTGGCATGGAGATAAGCCCCATGTGTGACAAGCACAATGCTTCCGT GGAAAAATCACAGGTGGGCTTCATAGACTATATTGTTCATCCCCTCTGGGAGACATGGGCAGACCTCGTC CACCCTGACGCCCAGGATATTTTGGACACTTTGGAGGACAATCGTGAATGGTACCAGAGCACAATCCCTC AGAGCCCCTCTCCTGCACCTGATGACCCAGAGGAGGGCCGGCAGGGTCAAACTGAGAAATTCCAGTTTGA ACTAACTTTAGAGGAAGATGGTGAGTCAGACACGGAAAAGGACAGTGGCAGTCAAGTGGAAGAAGACACT AGCTGCAGTGACTCCAAGACTCTTTGTACTCAAGACTCAGAGTCTACTGAAATTCCCCTTGATGAACAGG TTGAAGAGGAGGCAGTAGGGGAAGAAGAGGAAAGCCAGCCTGAAGCCTGTGTCATAGATGATCGTTCTCC TGACACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231369 representing NM_001197220
Red=Cloning site Green=Tags(s) MSIIMKPRSRSTSSLRTAEAVCFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYD LSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITE EAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDK QHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFR IAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLST PALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDI FQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCAD LSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLV HPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDT SCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001197220 |
ORF Size | 2037 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001197220.2 |
RefSeq ORF | 2040 bp |
Locus ID | 5144 |
UniProt ID | Q08499 |
Protein Families | Druggable Genome |
Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
MW | 77.3 kDa |
Gene Summary | This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231369L3 | Lenti-ORF clone of PDE4D (Myc-DDK-tagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 6 |
CNY 9,120.00 |
|
RC231369L4 | Lenti-ORF clone of PDE4D (mGFP-tagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 6 |
CNY 9,120.00 |
|
RG231369 | PDE4D (tGFP-tagged) - Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 6 |
CNY 9,056.00 |