BVES (NM_001199563) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CARICK; HBVES; LGMD2X; LGMDR25; POP1; POPDC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232575 representing NM_001199563 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTATACAGAGTCCAGCCCATTGAGAGAATCAACTGCCATAGGTTTTACACCTGAGTTAGAAAGTA TCATACCTGTGCCTTCCAATAAGACCACTTGTGAAAACTGGAGAGAGATACATCATCTGGTTTTTCATGT AGCAAATATTTGTTTTGCAGTTGGGTTGGTTATTCCAACTACTCTTCACCTTCATATGATATTTCTTAGG GGAATGTTAACTCTAGGATGTACCCTTTATATCGTCTGGGCCACTCTCTACCGATGTGCCTTGGATATAA TGATCTGGAACTCTGTGTTCTTGGGTGTCAACATTTTGCATCTGTCGTATCTTTTATACAAGAAGAGACC GGTAAAGATTGAAAAGGAACTCAGTGGCATGTACCGGCGATTGTTTGAACCACTCCGTGTGCCTCCAGAT TTGTTCAGAAGACTAACTGGACAGTTTTGCATGATCCAAACCTTGAAAAAGGGCCAAACTTATGCTGCAG AGGATAAAACCTCAGTTGATGACCGTCTGAGTATTCTCTTGAAGGGAAAAATGAAGGTCTCCTATCGAGG ACATTTTCTGCATAACATTTACCCCTGTGCCTTTATAGATTCTCCTGAATTTAGATCAACTCAGATGCAC AAAGGTGAAAAATTCCAGGTCACCATTATTGCAGATGATAACTGCAGATTTTTATGCTGGTCAAGAGAAA GATTAACATACTTTCTGGAATCAGAACCTTTCTTGTATGAAATCTTTAGGTATCTTATTGGAAAAGACAT CACAAATAAGCTCTACTCATTGAATGATCCCACCTTAAATGATAAAAAAGCCAAAAAGCTGGAACATCAG CTCAGCCTCTGCACACAGATCTCCATGTTGGAAATGAGGAACAGTATAGCCAGCTCCAGTGACAGTGACG ACGGCTTGCACCAGTTTCTTCGGGGTACCTCCAGCATGTCCTCTCTTCATGTGTCATCCCCACACCAGCG AGCCTCTGCCAAGATGAAACCGATAGAAGAAGGAGCAGAAGATGATGATGACGTTTTTGAACCGGCATCT CCAAATACATTGAAAGTCCATCAGCTGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232575 representing NM_001199563 Red=Cloning site Green=Tags(s) MNYTESSPLRESTAIGFTPELESIIPVPSNKTTCENWREIHHLVFHVANICFAVGLVIPTTLHLHMIFLR GMLTLGCTLYIVWATLYRCALDIMIWNSVFLGVNILHLSYLLYKKRPVKIEKELSGMYRRLFEPLRVPPD LFRRLTGQFCMIQTLKKGQTYAAEDKTSVDDRLSILLKGKMKVSYRGHFLHNIYPCAFIDSPEFRSTQMH KGEKFQVTIIADDNCRFLCWSRERLTYFLESEPFLYEIFRYLIGKDITNKLYSLNDPTLNDKKAKKLEHQ LSLCTQISMLEMRNSIASSSDSDDGLHQFLRGTSSMSSLHVSSPHQRASAKMKPIEEGAEDDDDVFEPAS PNTLKVHQLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199563
ORF Size	1080 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199563.2
RefSeq Size	5599 bp
RefSeq ORF	1083 bp
Locus ID	11149
UniProt ID	Q8NE79
Protein Families	Transmembrane
MW	41.5 kDa
Gene Summary	This gene encodes a member of the POP family of proteins containing three putative transmembrane domains. This gene is expressed in cardiac and skeletal muscle and may play an important role in development of these tissues. The mouse ortholog may be involved in the regeneration of adult skeletal muscle and may act as a cell adhesion molecule in coronary vasculogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

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