Kinesin Heavy Chain 2 (KIF2A) (NM_001243952) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDCBM3; HK2; KIF2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233079 representing NM_001243952 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGAATACATCAAGCAATGGTAACATCTTTAAATGAAGATAATGAAAGTGTAACTGTTGAATGGA TAGAAAATGGAGATACAAAAGGCAAAGAGATTGACCTGGAGAGCATCTTTTCACTTAACCCTGACCTTGT TCCTGATGAAGAAATTGAACCCAGTCCAGAAACACCTCCACCTCCAGCATCCTCAGCCAAAGTAAACAAA ATTGTAAAGAATCGACGGACTGTAGCTTCTATTAAGAATGACCCTCCTTCAAGAGATAATAGAGTGGTTG GTTCAGCACGTGCACGGCCCAGTCAATTTCCTGAACAGTCTTCCTCTGCACAACAGAATGGTAGTGTTTC AGATATATCTCCAGTTCAAGCTGCAAAAAAGGAATTTGGACCCCCTTCACGTAGAAAATCTAATTGTGTG AAAGAAGTAGAAAAACTGCAAGAAAAACGAGAGAAAAGGAGATTGCAACAGCAAGAACTTAGAGAAAAAA GAGCCCAGGACGTTGATGCTACAAACCCAAATTATGAAATTATGTGTATGATCAGAGACTTTAGAGGAAG TTTGGATTATAGACCATTAACAACAGCAGATCCTATTGATGAACATAGGATATGTGTGTGTGTAAGAAAA CGACCACTCAATAAAAAAGAAACTCAAATGAAAGATCTTGATGTAATCACAATTCCTAGTAAAGATGTTG TGATGGTACATGAACCAAAACAAAAAGTAGATTTAACAAGGTACCTAGAAAACCAAACATTTCGTTTTGA TTATGCCTTTGATGACTCAGCTCCTAATGAAATGGTTTACAGGTTTACTGCTAGACCACTAGTGGAAACT ATATTTGAAAGGGGAATGGCTACATGCTTTGCTTATGGGCAGACTGGAAGTGGAAAAACTCATACTATGG GTGGTGACTTTTCAGGAAAGAACCAAGATTGTTCTAAAGGAATTTATGCATTAGCAGCTCGAGATGTCTT TTTAATGCTAAAGAAGCCAAACTATAAGAAGCTAGAACTTCAAGTATATGCAACCTTCTTTGAAATTTAT AGTGGAAAGGTGTTTGACTTGCTAAACAGGAAAACAAAATTAAGAGTTCTAGAAGATGGAAAACAGCAGG TTCAAGTGGTGGGATTACAGGAACGGGAGGTCAAATGTGTTGAAGATGTACTGAAACTCATTGACATAGG CAACAGTTGCAGAACATCCGGTCAAACATCTGCAAATGCACATTCATCTCGGAGCCATGCAGTGTTTCAG ATTATTCTTAGAAGGAAAGGAAAACTACATGGCAAATTTTCTCTCATTGATTTGGCTGGAAATGAAAGAG GAGCTGATACTTCCAGTGCGGACAGGCAAACTAGGCTTGAAGGTGCTGAAATTAATAAAAGCCTTTTAGC ACTCAAGGAGTGCATCAGAGCCTTAGGTAGAAATAAACCTCATACTCCTTTCCGTGCAAGTAAACTCACT CAGGTGTTAAGAGATTCTTTCATAGGTGAAAACTCTCGTACCTGCATGATTGCCACAATCTCTCCAGGAA TGGCATCCTGTGAAAATACTCTTAATACATTAAGATATGCAAATAGGGTCAAAGAATTGACTGTAGATCC AACTGCTGCTGGTGATGTTCGTCCAATAATGCACCATCCACCAAACCAGATTGATGACTTAGAGACACAG TGGGGTGTGGGGAGTTCCCCTCAGAGAGATGATCTAAAACTTCTTTGTGAACAAAATGAAGAAGAAGTCT CTCCACAGTTGTTTACTTTCCACGAAGCTGTTTCACAAATGGTAGAAATGGAAGAACAAGTTGTAGAAGA TCACAGGGCAGTGTTCCAGGAATCTATTCGGTGGTTAGAAGATGAAAAGGCCCTCTTAGAGATGACTGAA GAAGTAGATTATGATGTCGATTCATATGCTACACAACTTGAAGCTATTCTTGAGCAAAAAATAGACATTT TAACTGAACTGCGGGATAAAGTGAAATCTTTCCGTGCAGCTCTACAAGAGGAGGAACAAGCCAGCAAGCA AATCAACCCGAAGAGACCCCGTGCCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233079 representing NM_001243952 Red=Cloning site Green=Tags(s) MGRIHQAMVTSLNEDNESVTVEWIENGDTKGKEIDLESIFSLNPDLVPDEEIEPSPETPPPPASSAKVNK IVKNRRTVASIKNDPPSRDNRVVGSARARPSQFPEQSSSAQQNGSVSDISPVQAAKKEFGPPSRRKSNCV KEVEKLQEKREKRRLQQQELREKRAQDVDATNPNYEIMCMIRDFRGSLDYRPLTTADPIDEHRICVCVRK RPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVDLTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVET IFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIY SGKVFDLLNRKTKLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQ IILRRKGKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLT QVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELTVDPTAAGDVRPIMHHPPNQIDDLETQ WGVGSSPQRDDLKLLCEQNEEEVSPQLFTFHEAVSQMVEMEEQVVEDHRAVFQESIRWLEDEKALLEMTE EVDYDVDSYATQLEAILEQKIDILTELRDKVKSFRAALQEEEQASKQINPKRPRAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243952
ORF Size	2058 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243952.1, NP_001230881.1
RefSeq Size	4261 bp
RefSeq ORF	2040 bp
Locus ID	3796
UniProt ID	O00139
Protein Families	Druggable Genome
MW	78.2 kDa
Gene Summary	The protein encoded by this gene is a plus end-directed motor required for normal mitotic progression. The encoded protein is required for normal spindle activity during mitosis and is necessary for normal brain development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

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