THADA (NM_001271643) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARMC13; GITA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233182 representing NM_001271643 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTAAAGAAGAAGAAAGAAATGCAAGTTGCTGCGCTGACCATTTGCCATCAGGACCTTGAAACTT TGAAATCTTTTGCTGATGTGGAAGGGAAAAATCTAGCTTCTTTGCTGTTACATTGTGTGCAACTCACGGA TGGAGTGTCACAAATCCATTATATTAAACAGATTGTGCCTCTGCTGGAGAAAGCAGATAAAAATGGCATG TGTGATCCCACTATTCAAAGTTGTTTGGATATCTTAGCAGGCATTTATCTTTCTTTGAGTCTAAAGAATC CCTTGAAGAAAGTATTGGCAAGCTCACTAAATAGCCTGCCTGATTTTTTTCTACCTGAGGCTATGCACCG TTTTACTTCTCGTCTTCAGGAAGAATTGAATACTACTGACTTATACTCTTACAGGAAAGTTACTGACAAT ATTTCTTCCTGTATGGAGAACTTTAACTTGGGTAGAGCAAGTGTTAATAATCTGCTTAAAAATGTGCTTC ATTTTCTGCAGAAGAGTTTAATTGAAATCCTGGAAGAAAATAGAAAATGTGCTGGAAATCATATTATTCA AACACAGTTGATGAATGACTTACTGGTAGGCATTAGAGTTTCAATGATGTTAGTACAGAAAGTACAAGAT TTCCAGGGAAATCTTTGGAAGACTTCCGATTCTCCCATATGGCAAAATATGTGTGGATTGCTGAGTATTT TTACCAAGGTTTTAAGCGATGATGATCTGTTACAGACTGTACAGAGCACATCTGGATTAGCTATTATTCT TTTTATTAAGACTATGTTTCACCCGTCTGAAAAGATTCCTCATTTGATTAGCAGTGTGCTGCTTCGTTCA GTGGACTGCACCAGTGTCCCCGAGTGGTTTATGAGCAGCTGCAGGAGCCTCTGTTGTGGTGACATCTCTC AGTCAGCTGTCTTATTCCTCTGTCAGGGGACACTTGCCATGTTGGACTGGCAGAACGGAAGCATGGGTCG GAGTGGGGAGGCCCTGCTCTTGGATACTGCACATGTTTTGTTCACCTTGAGTTCACAGATTAAAGAGCCA ACGCTGGAAATGTTTCTGTCTAGAATCTTAGCATCCTGGACTAATTCAGCCATACAAGTCCTTGAATCAA GTTCCCCGAGCCTAACGGACAGCCTGAATGGGAATTCAAGTATAGTTGGGAGACTTTTGGAATATGTCTA TACCCATTGGGAACATCCATTGGATGCTCTGAGACACCAAACCAAAATCATGTTCAAAAACCTTCTCCAA ATGCACCGGCTCACTGTGGAAGGTGCAGATTTCGTCCCTGATCCTTTCTTTGTGGAATTGACTGAGAGTC TTTTACGATTGGAATGGCATATTAAAGGAAAGTACACGTGCCTTGGTTGTTTGGTAGAGTGCATAGGAGT TGAACATATTTTGGCTATAGATAAAACTATTCCATCTCAAATCTTAGAGGTGATGGGAGACCAGTCATTG GTACCTTATGCAAGTGACCTCTTGGAAACCATGTTTAGAAATCATAAGAGTCATTTGAAATCCCAGACTG CTGAGAGTTCTTGGATTGACCAGTGGCATGAGACTTGGGTTTCTCCTCTCCTTTTTATATTGTGTGAAGG AAACTTGGATCAAAAATCTTACGTGATTGATTATTACTTGCCAAAATTATTAAGTTACAGCCCTGAAAGC TTACAGTACATGGTAAAGATTCTTCAGACTTCTATTGATGCTAAAACTGGACAAGAGCAATCTTTCCCAT CCTTAGGGTCTTGTAATAGCAGGGGGGCTCTGGGAGCTTTGATGGCATGTCTGCGAATAGCTAGAGCTCA TGGACATCTTCAGTCTGCAACTGATACCTGGGAGAACCTCGTGTCTGATGCAAGAATAAAGCAAGGCTTA ATTCATCAGCATTGCCAAGTAAGGATAGATACATTAGGCTTGCTTTGTGAAAGTAATCGGAGCACAGAAA TTGTTTCCATGGAAGAAATGCAGTGGATTCAGTTCTTTATTACATACAATCTTAACAGCCAGTCTCCAGG AGTGCGGCAACAGATCTGTTCTCTTCTTAAAAAGTTGTTTTGTAGGATACAGGAAAGTTCTCAGGTACTT TATAAATTGGAGCAGAGTAAATCCAAACGTGAACCAGAGAATGAGTTAACCAAACAGCACCCTTCTGTTT CTTTACAGCAGTATAAGAATTTCATGTCATCCATTTGTAACAGTCTTTTTGAAGCATTGTTTCCTGGATC TTCCTACTCGACTAGATTTTCAGCTTTAACCATTTTAGGTTCAATAGCTGAAGTTTTTCATGTCCCAGAA GGCAGAATTTATACAGTATATCAGCTGAGTCATGATATTGATGTTGGTCGTTTCCAAACACTAATGGAAT GTTTTACCAGCACTTTTGAAGACGTGAAAATTTTAGCATTTGATCTTCTGATGAAGTTATCAAAAACAGC TGTACATTTTCAGGATTCGGGGAAACTGCAAGGCTTATTTCAGGCAGCATTGGAGCTCAGCACAAGCACC AAACCATACGACTGTGTGACAGCTTCCTACCTGCTGAACTTCTTAATCTGGCAGGATGCTCTACCGTCAT CCTTGTCTGCCTACTTAACTCAGCAAGTTGCATGTGATAATGGAGATAGGCCTGCTGCTGTGGTGGAAAG GAACACATTAATGGTTATCAAATGCTTGATGGAAAATCTTGAGGAAGAAGTATCTCAGGCTGAAAATTCT CTGCTTCAGGCAGCAGCAGCATTTCCAATGTATGGGCGAGTCCACTGTATAACAGGAGCTTTGCAGAAGT TATCTCTAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233182 representing NM_001271643 Red=Cloning site Green=Tags(s) MGVKKKKEMQVAALTICHQDLETLKSFADVEGKNLASLLLHCVQLTDGVSQIHYIKQIVPLLEKADKNGM CDPTIQSCLDILAGIYLSLSLKNPLKKVLASSLNSLPDFFLPEAMHRFTSRLQEELNTTDLYSYRKVTDN ISSCMENFNLGRASVNNLLKNVLHFLQKSLIEILEENRKCAGNHIIQTQLMNDLLVGIRVSMMLVQKVQD FQGNLWKTSDSPIWQNMCGLLSIFTKVLSDDDLLQTVQSTSGLAIILFIKTMFHPSEKIPHLISSVLLRS VDCTSVPEWFMSSCRSLCCGDISQSAVLFLCQGTLAMLDWQNGSMGRSGEALLLDTAHVLFTLSSQIKEP TLEMFLSRILASWTNSAIQVLESSSPSLTDSLNGNSSIVGRLLEYVYTHWEHPLDALRHQTKIMFKNLLQ MHRLTVEGADFVPDPFFVELTESLLRLEWHIKGKYTCLGCLVECIGVEHILAIDKTIPSQILEVMGDQSL VPYASDLLETMFRNHKSHLKSQTAESSWIDQWHETWVSPLLFILCEGNLDQKSYVIDYYLPKLLSYSPES LQYMVKILQTSIDAKTGQEQSFPSLGSCNSRGALGALMACLRIARAHGHLQSATDTWENLVSDARIKQGL IHQHCQVRIDTLGLLCESNRSTEIVSMEEMQWIQFFITYNLNSQSPGVRQQICSLLKKLFCRIQESSQVL YKLEQSKSKREPENELTKQHPSVSLQQYKNFMSSICNSLFEALFPGSSYSTRFSALTILGSIAEVFHVPE GRIYTVYQLSHDIDVGRFQTLMECFTSTFEDVKILAFDLLMKLSKTAVHFQDSGKLQGLFQAALELSTST KPYDCVTASYLLNFLIWQDALPSSLSAYLTQQVACDNGDRPAAVVERNTLMVIKCLMENLEEEVSQAENS LLQAAAAFPMYGRVHCITGALQKLSLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271643
ORF Size	2811 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271643.2
RefSeq Size	3313 bp
RefSeq ORF	2814 bp
Locus ID	63892
UniProt ID	Q6YHU6
MW	105.8 kDa
Gene Summary	This gene is the target of 2p21 choromosomal aberrations in benign thyroid adenomas. Single nucleotide polymorphisms (SNPs) in this gene may be associated with type 2 diabetes and polycystic ovary syndrome. The encoded protein is likely involved in the death receptor pathway and apoptosis. [provided by RefSeq, Sep 2016]

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