RGS6 (NM_001204423) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GAP; HA117; S914
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234091 representing NM_001204423 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGATGACAAGACAGGGGGTGTGCCCATCAGAACAGTCAAGAGCTTTCTCTCCAAAATCCCCAGTG TCGTCACAGGTACTGACATTGTGCAGTGGCTTATGAAGAACCTTTCCATTGAGGACCCAGTTGAAGCAAT ACACTTGGGGAGCCTTATCGCTGCCCAGGGCTACATCTTTCCAATCTCAGACCATGTTCTCACCATGAAG GATGATGGCACCTTTTATCGTTTCCAGGCTCCGTACTTCTGGCCTTCGAACTGCTGGGAACCTGAAAACA CTGACTATGCCATCTATCTCTGTAAGAGGACAATGCAAAATAAAGCAAGGCTGGAACTGGCAGATTATGA AGCAGAAAACTTAGCAAGACTCCAGAGGGCCTTTGCGAGGAAGTGGGAATTCATCTTTATGCAAGCAGAA GCACAAGTAAAGATTGACCGGAAAAAAGACAAGACAGAAAGGAAAATTTTGGATAGTCAAGAACGAGCCT TTTGGGATGTCCACAGGCCTGTGCCAGGCTGTGTGAACACAACAGAAATGGATATCCGAAAATGTCGACG TTTGAAGAATCCACAAAAGGTTAAAAAGTCCGTGTATGGCGTGACTGAAGAGTCCCAGGCACAGAGCCCG GTGCATGTACTCAGCCAACCAATCAGGAAAACAACAAAAGAGGACATCCGGAAACAGATAACATTTTTGA ACGCACAGATCGACAGACATTGTTTGAAAATGTCCAAAGTGGCTGAAAGTTTAATTGCCTACACGGAACA ATATGTGGAATATGACCCTTTGATAACACCAGCTGAGCCATCCAACCCTTGGATCAGCGATGACGTTGCT TTGTGGGACATAGAGATGAGCAAAGAGCCCAGCCAACAGCGAGTAAAAAGATGGGGCTTCTCTTTCGATG AGATATTGAAGGACCAGGTGGGGCGGGACCAGTTTCTACGATTCCTGGAGTCCGAATTCAGTTCAGAAAA CCTCAGGTTCTGGCTGGCTGTCCAAGATCTTAAGAAACAACCCCTACAGGATGTGGCCAAGAGGGTAGAA GAAATCTGGCAAGAGTTTCTGGCTCCAGGGGCTCCAAGTGCAATCAACCTGGATTCTCACAGCTATGAGA TAACCAGTCAAAATGTCAAAGATGGAGGGAGATATACATTTGAAGACGCCCAGGAGCACATCTACAAGCT GATGAAGAGTGACAGCTATGCCCGCTTCCTCCGGTCAAATGCTTACCAGGATTTGCTGCTGGCCAAGAAG AAGGGAAAGTCGCTGGCGGGCAAGCGCCTCACGGGCCTGATGCAGTCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234091 representing NM_001204423 Red=Cloning site Green=Tags(s) MQDDKTGGVPIRTVKSFLSKIPSVVTGTDIVQWLMKNLSIEDPVEAIHLGSLIAAQGYIFPISDHVLTMK DDGTFYRFQAPYFWPSNCWEPENTDYAIYLCKRTMQNKARLELADYEAENLARLQRAFARKWEFIFMQAE AQVKIDRKKDKTERKILDSQERAFWDVHRPVPGCVNTTEMDIRKCRRLKNPQKVKKSVYGVTEESQAQSP VHVLSQPIRKTTKEDIRKQITFLNAQIDRHCLKMSKVAESLIAYTEQYVEYDPLITPAEPSNPWISDDVA LWDIEMSKEPSQQRVKRWGFSFDEILKDQVGRDQFLRFLESEFSSENLRFWLAVQDLKKQPLQDVAKRVE EIWQEFLAPGAPSAINLDSHSYEITSQNVKDGGRYTFEDAQEHIYKLMKSDSYARFLRSNAYQDLLLAKK KGKSLAGKRLTGLMQSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204423
ORF Size	1311 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204423.2
RefSeq Size	5546 bp
RefSeq ORF	1314 bp
Locus ID	9628
UniProt ID	P49758
Protein Families	Druggable Genome
MW	51.1 kDa
Gene Summary	This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP and GGL domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Many alternatively spliced transcript variants encoding different isoforms with long or short N-terminal domains, complete or incomplete GGL domains, and distinct C-terminal domains, have been described for this gene, however, the full-length nature of some of these variants is not known.[provided by RefSeq, Mar 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.