ZNF266 (NM_001271314) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HZF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234417 representing NM_001271314 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGAGAACTACAAGAATTTGGCCACAGTAGGATATCAGCTCTTCAAACCCAGTCTGATCTCTTGGC TGGAACAAGAAGAGTCTAGGACAGTGCAGAGAGGTGATTTCCAAGCTTCAGAATGGAAAGTGCAACTTAA AACCAAAGAGTTAGCCCTTCAGCAGGATGTTTTGGGGGAGCCAACCTCCAGTGGGATTCAAATGATAGGA AGCCACAACGGAGGGGAGGTCAGTGATGTTAAGCAATGTGGAGATGTCTCCAGTGAACACTCATGCCTTA AGACACATGTGAGAACTCAAAATAGTGAGAACACATTTGAGTGTTATCTGTATGGAGTAGACTTCCTTAC TCTGCACAAGAAAACCTCTACTGGAGAGCAACGTTCTGTATTTAGTCAGTGTGGAAAAGCCTTCAGCCTG AACCCAGATGTTGTTTGCCAGAGAACGTGCACAGGAGAGAAAGCTTTTGATTGCAGTGACTCTGGGAAAT CCTTCATTAATCATTCACACCTTCAGGGACATTTAAGAACTCACAATGGAGAAAGTCTCCATGAATGGAA GGAATGTGGGAGAGGCTTTATTCACTCCACAGACCTTGCTGTGCGTATACAAACTCACAGGTCAGAAAAA CCCTACAAATGTAAGGAATGTGGAAAAGGATTTAGATATTCTGCATACCTTAATATTCACATGGGAACCC ACACTGGAGACAATCCCTATGAGTGTAAGGAGTGTGGGAAAGCCTTCACCAGGTCTTGTCAACTTACTCA GCACAGAAAAACTCACACTGGAGAGAAACCTTATAAATGTAAGGATTGTGGGAGAGCCTTCACTGTTTCC TCTTGCTTAAGTCAACATATGAAAATCCATGTGGGTGAGAAGCCTTATGAATGCAAGGAATGTGGGATAG CCTTCACTAGATCTTCTCAACTTACTGAACATTTAAAAACTCACACTGCAAAGGATCCCTTTGAATGTAA GATATGTGGAAAATCCTTTAGAAATTCCTCATGCCTCAGTGATCACTTTCGAATTCACACTGGAATAAAA CCCTATAAATGTAAGGATTGTGGGAAAGCCTTCACTCAGAACTCAGACCTTACTAAGCATGCACGAACTC ACAGTGGAGAGAGGCCCTATGAATGTAAGGAATGTGGAAAGGCCTTTGCCAGATCCTCTCGCCTTAGTGA ACATACAAGAACTCACACTGGAGAGAAGCCTTTTGAATGTGTCAAATGTGGGAAAGCCTTTGCTATTTCT TCAAATCTTAGTGGACATTTGAGAATTCACACTGGAGAGAAGCCCTTTGAGTGCCTGGAATGTGGTAAAG CATTTACGCATTCCTCCAGTCTTAATAATCACATGCGGACCCACAGCGCCAAAAAACCATTCACGTGTAT GGAATGTGGCAAAGCTTTTAAGTTTCCCACGTGTGTTAACCTTCACATGCGGATCCACACTGGAGAAAAA CCCTACAAATGTAAACAGTGTGGGAAATCCTTCAGTTACTCCAATTCGTTTCAGTTACATGAACGAACTC ACACTGGAGAGAAACCCTATGAATGTAAGGAGTGCGGGAAAGCCTTCAGTTCTTCCAGTTCCTTTCGAAA TCATGAAAGAAGGCATGCGGATGAGAGACTGTCAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234417 representing NM_001271314 Red=Cloning site Green=Tags(s) MLENYKNLATVGYQLFKPSLISWLEQEESRTVQRGDFQASEWKVQLKTKELALQQDVLGEPTSSGIQMIG SHNGGEVSDVKQCGDVSSEHSCLKTHVRTQNSENTFECYLYGVDFLTLHKKTSTGEQRSVFSQCGKAFSL NPDVVCQRTCTGEKAFDCSDSGKSFINHSHLQGHLRTHNGESLHEWKECGRGFIHSTDLAVRIQTHRSEK PYKCKECGKGFRYSAYLNIHMGTHTGDNPYECKECGKAFTRSCQLTQHRKTHTGEKPYKCKDCGRAFTVS SCLSQHMKIHVGEKPYECKECGIAFTRSSQLTEHLKTHTAKDPFECKICGKSFRNSSCLSDHFRIHTGIK PYKCKDCGKAFTQNSDLTKHARTHSGERPYECKECGKAFARSSRLSEHTRTHTGEKPFECVKCGKAFAIS SNLSGHLRIHTGEKPFECLECGKAFTHSSSLNNHMRTHSAKKPFTCMECGKAFKFPTCVNLHMRIHTGEK PYKCKQCGKSFSYSNSFQLHERTHTGEKPYECKECGKAFSSSSSFRNHERRHADERLSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271314
ORF Size	1647 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271314.1, NP_001258243.1
RefSeq Size	3739 bp
RefSeq ORF	1650 bp
Locus ID	10781
UniProt ID	Q14584
Protein Families	Transcription Factors
MW	62.1 kDa
Gene Summary	This gene encodes a protein containing many tandem zinc-finger motifs. Zinc fingers are protein or nucleic acid-binding domains, and may be involved in a variety of functions, including regulation of transcription. This gene is located in a cluster of similar genes encoding zinc finger proteins on chromosome 19. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Sep 2012]

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