DLGAP1 (NM_001242762) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; DLGAP1A; DLGAP1B; GKAP; SAPAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234665 representing NM_001242762 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTGAAGACATTAAAACTGTTTAATTCTCAGTTGAGATGTGGTTGGCTCTTATACATTTGGAATA AAGCATTTATGGCACATCTCAGGGTTCCACAAGATGAATGGACAGGGTACACCCCACGAGGTAAAGATGA TGAAATTCCATGCCGAAGAATGCGGAGTGGCAGTTATATCAAGGCCATGGGGGATGAAGACAGTGGAGAC TCAGACACGAGTCCTAAGCCTTCTCCAAAAGTTGCTGCGCGGAGAGAAAGCTATCTCAAGGCTACTCAGC CATCCCTTACAGAACTCACCACACTCAAAATCTCCAATGAACACTCACCCAAACTCCAGATCCGGAGTCA TAGTTACCTGAGGGCAGTGAGTGAAGTCTCCATCAACCGGAGCCTGGACAGCCTGGACCCTGCAGGCTTG CTCACATCACCAAAGTTCCGCTCCAGGAATGAGAGCTACATGCGAGCCATGAGCACCATCAGCCAGGTGA GCGAGATGGAAGTGAACGGGCAGTTCGAGTCCGTGTGCGAGTCCGTGTTCAGCGAGCTGGAGTCGCAGGC CGTGGAAGCGCTGGACCTGCCCATGCCCGGCTGCTTCCGCATGCGGAGCCACAGCTATGTGCGGGCCATT GAGAAAGGCTGCTCCCAGGACGACGAGTGCGTGTCCCTGAGGTCGTCCTCGCCGCCGCGCACCACCACCA CCGTTAGGACCATCCAGAGCAGCACGGCCCAGAGTAGCACAGAGTCAGCCCAGGATGCCTACATGGACGG ACAGGGCCAGCGAGGAGATATTATCAGCCAGTCTGGACTCAGCAACTCCACCGAGAGCCTGGACAGTATG AAGGCTCTGACAGCCGCCATCGAAGCTGCAAACGCCCAGATCCATGGCCCTGCCAGTCAACACATGGGCA ATAACACTGCCACCGTCACCACCACGACTACCATAGCCACCGTCACCACGGAGGACAGGAAGAAGGACCA CTTTAAGAAAAATCGATGCCTGTCTATCGGGATACAGGTGGATGATGCTGAAGAACCTGACAAAACAGGG GAGAATAAAGCACCCAGTAAGTTCCAGTCCGTGGGAGTGCAAGTAGAAGAAGAGAAGTGCTTCCGCAGGT TCACTCGATCCAACAGTGTGACGACAGCAGTACAGGCCGACCTGGACTTCCATGATAATCTGGAAAATTC TCTGGAATCTATAGAGGACAATTCGTGTCCTGGCCCCATGGCCAGACAGTTCTCCCGCGATGCCAGCACC TCCACAGTCAGCATTCAGGGCTCAGGAAACCATTACCATGCCTGTGCCGCCGATGATGACTTTGACACGG ATTTTGACCCCTCTATTCTGCCTCCTCCGGACCCCTGGATTGACTCTATCACTGAAGACCCTCTGGAGGC CGTGCAAAGGTCAGTGTGCCACCGGGATGGCCACTGGTTCCTGAAGCTTCTCCAGGCAGAGCGAGACCGC ATGGAGGGGTGGTGTCAACAGATGGAGCGGGAAGAACGGGAAAACAACCTGCCCGAAGACATTCTAGGAA AAATCCGAACCGCAGTGGGCAGTGCCCAACTTCTCATGGCCCAGAAATTCTACCAGTTCAGAGAACTGTG TGAAGAAAACCTGAATCCTAATGCTCATCCAAGACCCACCTCCCAGGATTTGGCGGGGTTTTGGGACATG CTGCAGTTGTCCATAGAAAATATTAGTATGAAATTTGATGAACTTCATCAGTTAAAGGCCAATAATTGGA AACAGATGGATCCTCTTGACAAGAAGGAGAGAAGGGCCCCTCCTCCAGTGCCAAAGAAGCCGGCGAAGGG CCCCGCGCCGCTGATCCGGGAGCGCTCGCTGGAGAGCTCGCAGCGCCAGGAGGCCCGCAAGCGCCTGATG GCCGCCAAGCGCGCCGCGTCCGTCCGCCAGAACTCGGCCACCGAGAGCGCCGAGAGCATCGAGATCTACA TCCCCGAGGCGCAGACCCGGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234665 representing NM_001242762 Red=Cloning site Green=Tags(s) MDLKTLKLFNSQLRCGWLLYIWNKAFMAHLRVPQDEWTGYTPRGKDDEIPCRRMRSGSYIKAMGDEDSGD SDTSPKPSPKVAARRESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEVSINRSLDSLDPAGL LTSPKFRSRNESYMRAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPMPGCFRMRSHSYVRAI EKGCSQDDECVSLRSSSPPRTTTTVRTIQSSTAQSSTESAQDAYMDGQGQRGDIISQSGLSNSTESLDSM KALTAAIEAANAQIHGPASQHMGNNTATVTTTTTIATVTTEDRKKDHFKKNRCLSIGIQVDDAEEPDKTG ENKAPSKFQSVGVQVEEEKCFRRFTRSNSVTTAVQADLDFHDNLENSLESIEDNSCPGPMARQFSRDAST STVSIQGSGNHYHACAADDDFDTDFDPSILPPPDPWIDSITEDPLEAVQRSVCHRDGHWFLKLLQAERDR MEGWCQQMEREERENNLPEDILGKIRTAVGSAQLLMAQKFYQFRELCEENLNPNAHPRPTSQDLAGFWDM LQLSIENISMKFDELHQLKANNWKQMDPLDKKERRAPPPVPKKPAKGPAPLIRERSLESSQRQEARKRLM AAKRAASVRQNSATESAESIEIYIPEAQTRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242762
ORF Size	1983 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242762.2
RefSeq Size	5214 bp
RefSeq ORF	1986 bp
Locus ID	9229
UniProt ID	O14490
MW	74.5 kDa
Gene Summary	Part of the postsynaptic scaffold in neuronal cells.[UniProtKB/Swiss-Prot Function]

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