PIWIL3 (NM_001255975) Human Tagged ORF Clone
CAT#: RC234982
- TrueORF®
PIWIL3 (Myc-DDK tagged) - Homo sapiens piwi-like RNA-mediated gene silencing 3 (PIWIL3), transcript variant 2
ORF Plasmid: tGFP
"NM_001255975" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HIWI3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234982 representing NM_001255975
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGTAGGGCAAGGACTCGCGCCCGAGGCAGAGCCCGCCGCAGGGAGAGCTACCAACAAGAGGCAC CTGGGGGACCCAGAGCACCTGGATCAGCTACAACCCAGGAGCCCCCTCAGTTGCAGTCGACACCCCGGCC GCTGCAGGAGGAAGTCCCAGTGGTTAGACCTCTGCAGCCAAGAGCAGCAAGAGGAGGAGCAGGAGGAGGA GCACAGTCTCAAGGGGTGAAGGAACCTGGACCTGAGGCTGGGTTGCATACAGCGCCCTTGCAGGAGAGAA GGATTGGTGGAGTTTTTCAAGACCTGGTGGTGAACACCAGGCAAGATATGAAGCATGTTAAAGACTCAAA AACAGGTTCAGAGGGTACAGTGGTACAGCTACTCGCCAACCACTTCCGAGTGATATCTCGTCCTCAGTGG GTTGCATATAAATACAACGTTGACTACAAACCAGACATAGAAGATGGAAATCTCCGTACAATTTTACTTG ATCAACATAGAAGGAAATTTGGAGAGCGCCATATATTTGATGGAAACTCTTTATTATTATCTCGGCCACT AAAAGAGCGGAGAGTGGAATGGTTGAGCACAACCAAAGACAAAAACATCGTGAAGATTACAGTTGAGTTT TCCAAAGAACTCACGCCCACGTCGCCAGATTGCCTACGCTATTACAACATTCTCTTTAGAAGAACTTTCA AGCTGCTGGATTTTGAACAAGTTGGTCGCAACTATTATACCAAAAAGAAGGCCATTCAGTTATACCGTCA TGGTACCAGTTTGGAAATCTGGCTTGGTTATGTTACTTCTGTTCTTCAATACGAAAACAGCATTACCCTC TGTGCCGATGTGAGCCACAAACTGCTCCGAATAGAAACTGCTTATGATTTCATAAAGAGAACATCTGCCC AGGCCCAGACAGGAAACATCCGAGAGGAAGTAACTAATAAATTAATTGGATCAATTGTTCTGACAAAATA CAACAACAAAACCTACAGAGTAGATGATATTGATTGGAAGCAGAATCCTGAAGACACATTTAACAAATCA GATGGCAGCAAAATCACCTATATAGACTACTACAGGCAGCAACATAAAGAAATTGTCACAGTGAAGAAAC AGCCACTTTTGGTCAGCCAGGGCAGATGGAAAAAGGGCCTAACGGGTACACAACGTGAACCTATCCTGCT GATTCCTCAGCTGTGCCACATGACAGGTCTAACAGATGAAATATGTAAAGATTATAGCATTGTGAAAGAA TTGGCTAAACATACAAGATTGAGTCCAAGAAGAAGGCATCATACATTAAAAGAATTCATCAATACTCTAC AAGATAATAAAAAAGTACGAGAGTTACTTCAACTCTGGGATTTGAAATTTGATACCAATTTTTTGTCCGT CCCGGGAAGAGTTTTGAAAAACGCAAACATCGTGCAAGGCAGAAGAATGGTTAAAGCCAATTCACAAGGA GACTGGTCAAGAGAAATAAGAGAATTACCCTTACTTAATGCAATGCCACTACATAGTTGGCTCATACTCT ATAGCAGGAGCAGTCACAGAGAAGCCATGTCCTTAAAGGGTCATCTACAGAGTGTCACAGCCCCCATGGG CATAACTATGAAACCAGCAGAAATGATTGAAGTAGATGGTGATGCTAACTCCTATATAGACACATTACGG AAATATACTAGACCAACACTGCAGATGGTGATTTGTATCCTGCCCAATGATGACAAACGTAGATATGACA GCATAAAAAGATACCTATGTACCAAATGCCCAATTCCAAGCCAGTGTGTGGTGAAAAAGACCTTAGAAAA AGTCCAGGCAAGGACCATCGTCACCAAGATTGCCCAGCAGATGAATTGCAAGATGGGAGGAGCCCTCTGG AAGGTGGAGACAGACGTACAAAGAACAATGTTCGTTGGCATTGATTGTTTCCACGATATCGTAAATCGAC AGAAATCAATAGCAGGATTTGTTGCAAGTACCAATGCTGAATTAACAAAGTGGTACTCTCAATGTGTCAT CCAGAAAACAGGAGAAGAGCTTGTGAAAGAGCTGGAGATCTGCTTGAAAGCTGCCCTGGATGTCTGGTGT AAAAACGAATCATCGATGCCACATTCTGTTATTGTGTATCGGGATGGAGTGGGAGATGGTCAGCTTCAAG CATTGCTTGACCATGAAGCGAAAAAGATGTCGACCTACTTAAAAACCATCTCTCCTAACAATTTCACTCT AGCTTTCATTGTGGTGAAGAAACGAATAAACACTAGATTTTTTCTTAAACATGGAAGCAATTTTCAAAAT CCACCTCCAGGAACAGTTATTGATGTAGAGTTGACTAGGAATGAATGGTATGACTTTTTTATTGTGAGTC AGTCTGTGCAAGATGGGACTGTTACCCCCACTCATTATAACGTCATCTATGACACGATTGGCTTGAGCCC AGATACAGTACAGCGTTTAACATATTGTCTATGCCACATGTATTATAATTTGCCAGGCATCATCCGAGTT CCAGCGCCTTGCCACTATGCCCACAAGCTGGCTTACCTCGTGGGGCAGTCCATTCACCAGGAACCGAATC GTTCCTTGTCAACTCGTCTCTTTTACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234982 representing NM_001255975
Red=Cloning site Green=Tags(s) MPGRARTRARGRARRRESYQQEAPGGPRAPGSATTQEPPQLQSTPRPLQEEVPVVRPLQPRAARGGAGGG AQSQGVKEPGPEAGLHTAPLQERRIGGVFQDLVVNTRQDMKHVKDSKTGSEGTVVQLLANHFRVISRPQW VAYKYNVDYKPDIEDGNLRTILLDQHRRKFGERHIFDGNSLLLSRPLKERRVEWLSTTKDKNIVKITVEF SKELTPTSPDCLRYYNILFRRTFKLLDFEQVGRNYYTKKKAIQLYRHGTSLEIWLGYVTSVLQYENSITL CADVSHKLLRIETAYDFIKRTSAQAQTGNIREEVTNKLIGSIVLTKYNNKTYRVDDIDWKQNPEDTFNKS DGSKITYIDYYRQQHKEIVTVKKQPLLVSQGRWKKGLTGTQREPILLIPQLCHMTGLTDEICKDYSIVKE LAKHTRLSPRRRHHTLKEFINTLQDNKKVRELLQLWDLKFDTNFLSVPGRVLKNANIVQGRRMVKANSQG DWSREIRELPLLNAMPLHSWLILYSRSSHREAMSLKGHLQSVTAPMGITMKPAEMIEVDGDANSYIDTLR KYTRPTLQMVICILPNDDKRRYDSIKRYLCTKCPIPSQCVVKKTLEKVQARTIVTKIAQQMNCKMGGALW KVETDVQRTMFVGIDCFHDIVNRQKSIAGFVASTNAELTKWYSQCVIQKTGEELVKELEICLKAALDVWC KNESSMPHSVIVYRDGVGDGQLQALLDHEAKKMSTYLKTISPNNFTLAFIVVKKRINTRFFLKHGSNFQN PPPGTVIDVELTRNEWYDFFIVSQSVQDGTVTPTHYNVIYDTIGLSPDTVQRLTYCLCHMYYNLPGIIRV PAPCHYAHKLAYLVGQSIHQEPNRSLSTRLFYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001255975 |
ORF Size | 2619 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001255975.1, NP_001242904.1 |
RefSeq Size | 3481 bp |
RefSeq ORF | 2622 bp |
Locus ID | 440822 |
Protein Pathways | Dorso-ventral axis formation |
MW | 100.6 kDa |
Gene Summary | This gene encodes a member of the PIWI subfamily of Argonaute family proteins. This subfamily of proteins contains a PAZ domain, found in proteins involved in RNA-mediated gene silencing, and a C-terminal Piwi domain. The encoded protein is thought to function in maintenance of germline cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
Product Manuals |
FAQs |
SDS |