AKAP1 (NM_001242903) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AKAP; AKAP84; AKAP121; AKAP149; D-AKAP1; PPP1R43; PRKA1; SAKAP84; TDRD17
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235013 representing NM_001242903. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAATCCAGTTCCGTTCGCTCTTCCCCTTGGCATTGCCTGGGATGCTGGCGCTCCTCGGCTGGTGG TGGTTTTTCTCTCGTAAAAAAGGCCATGTCAGCAGCCATGATGAGCAGCAGGTGGAGGCTGGTGCTGTG CAGCTGAGGGCTGACCCTGCCATCAAGGAACCTCTCCCCGTGGAAGACGTCTGTCCCAAAGTAGTGTCC ACACCCCCCAGTGTCACAGAGCCTCCAGAAAAGGAACTGTCCACCGTGAGCAAGCTGCCTGCAGAGCCC CCAGCATTGCTCCAGACACACCCACCTTGCCGAAGATCAGAGTCCTCGGGCATTCTTCCTAACACCACA GACATGAGATTGCGACCAGGAACACGCAGAGATGACAGTACAAAGCTGGAGCTAGCCCTGACAGGTGGT GAAGCCAAATCGATTCCTCTAGAGTGCCCCCTTTCATCCCCAAAGGGTGTACTATTCTCCAGCAAATCA GCTGAGGTGTGTAAGCAAGATTCCCCCTTCAGCAGGGTGCCAAGGAAGGTCCAGCCAGGCTACCCCGTA GTCCCCGCAGAGAAGCGTAGCTCTGGGGAGAGGGCAAGAGAGACAGGTGGGGCCGAAGGGACTGGTGAT GCCGTGTTGGGGGAAAAGGTGCTTGAAGAAGCTCTGTTGTCTCGGGAGCATGTCTTGGAATTGGAGAAC AGCAAGGGCCCCAGCCTGGCCTCTTTAGAGGGGGAAGAAGATAAGGGGAAGAGCAGCTCATCCCAGGTG GTGGGGCCAGTGCAGGAGGAAGAGTATGTAGCAGAGAAGTTGCCAAGTAGGTTCATCGAGTCGGCTCAC ACAGAGCTGGCAAAGGACGATGCGGCGCCAGCACCCCCAGTCGCAGACGCCAAAGCCCAGGATAGAGGT GTCGAGGGAGAACTGGGCAATGAGGAGAGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAG GGCTTGGATAGAAATGAGGAGAGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGATTAAG CGGGCTGCCTTCCAGATAATCTCCCAAGTGATCTCAGAAGCAACCGAACAGGTGCTGGCCACCACGGTT GGCAAGGTTGCAGGTCGTGTGTGTCAGGCCAGTCAGCTCCAAGGGCAGAAGGAAGAGAGCTGTGTCCCA GTTCACCAGAAAACTGTCTTGGGCCCAGACACTGCGGAGCCTGCCACAGCAGAGGCAGCTGTTGCCCCG CCGGATGCTGGCCTCCCCTTGCCAGGCCTACCAGCAGAGGGCTCACCACCACCAAAGACCTACGTGAGC TGCCTGAAGAGCCTTCTGTCCAGCCCCACCAAGGACAGTAAGCCAAATATCTCTGCACACCACATCTCC CTGGCCTCCTGCCTGGCACTGACCACCCCCAGTGAAGAGTTGCCGGACCGGGCAGGCATCCTGGTGGAA GATGCCACCTGTGTCACCTGCATGTCAGACAGCAGCCAAAGTGTCCCTTTGGTGGCTTCTCCAGGACAC TGCTCAGATTCTTTCAGCACTTCAGGGCTTGAAGACTCTTGCACAGAGACCAGCTCGAGCCCCAGGGAC AAGGCCATCACCCCGCCACTGCCAGAAAGTACTGTGCCCTTCAGCAATGGGGTGCTGAAGGGGGAGTTG TCAGACTTGGGGGCTGAGGATGGATGGACCATGGATGCGGAAGCAGATCATTCAGGAGGTTCTGACAGG AACAGCATGGATTCCGTGGATAGCTGTTGCAGTCTCAAGAAGACTGAGAGCTTCCAAAATGCCCAGGCA GGCTCCAACCCTAAGAAGGTCGACCTCATCATCTGGGAGATCGAGGTGCCAAAGCACTTAGTCGGTCGG CTAATTGGCAAGCAGGGGCGCTATGTGAGTTTTCTGAAGCAAACATCTGGTGCCAAGATCTACATTTCA ACCCTGCCTTACACCCAGAGCGTCCAGATCTGCCACATAGAAGGCTCTCAACATCATGTAGACAAAGCG CTGAACTTGATTGGGAAGAAGTTCAAAGAGCTGAACCTCACCAATATCTACGCTCCCCCATTGCCTTCA CTGGCACTGCCTTCTCTGCCGATGACATCCTGGCTCATGCTGCCTGATGGCATCACCGTGGAGGTCATT GTGGTCAACCAGGTCAATGCCGGGCACCTGTTCGTGCAGCAGCACACACACCCTACCTTCCACGCGCTG CGCAGCCTCGACCAGCAGATGTACCTCTGTTACTCTCAGCCTGGAATCCCCACCTTGCCCACCCCAGTG GAAATAACGGTCATCTGTGCCGCCCCTGGTGCGGACGGGGCCTGGTGGCGAGCCCAAGTGGTTGCCTCC TACGAGGAGACCAACGAAGTGGAGATTCGATACGTGCACTACGGCGGATATAAGAGGGTGAAAGTAGAC GTGCTCCGGCAAATCAGGTCTGACTTTGTCACCCTGCCGTTTCAGGGAGCAGAAGTCCTTCTGGACAGT GTGATGCCCCTGTCAGACGATGACCAGTTTTCACCGGAAGCAGATGCCGCCATGAGCGAGATGACGGGG AATACAGCACTGCTTGCTCAGGTGACAAGTTACAGTCCAACTGGTCTTCCTCTGATTCAGCTGTGGAGT GTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCCCTGGTGGAGCGAGGCCTTGCCCAGTGGGTAGAC AGCTACTACACAAGCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC235013 Blue=ORF Red=Cloning site Green=Tag(s) MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVS TPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGG EAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGD AVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAH TELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIK RAAFQIISQVISEATEQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAP PDAGLPLPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGILVE DATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPESTVPFSNGVLKGEL SDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGR LIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPS LALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPV EITVICAAPGADGAWWRAQVVASYEETNEVEIRYVHYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDS VMPLSDDDQFSPEADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVD SYYTSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242903
ORF Size	2709 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	4321 bp
RefSeq ORF	2712 bp
Locus ID	8165
UniProt ID	Q92667
Protein Families	Druggable Genome, Transmembrane
MW	97.4 kDa
Gene Summary	The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008]

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