POM121 (NM_001257190) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	P145; POM121A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235113 representing NM_001257190 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTGTAGCCCAGTGACTGTGAGGATCGCCCCTCCTGACAGAAGATTTTCGCGTTCTGCGATACCAG AGCAGATAATCAGCTCAACACTGTCCTCACCATCAAGTAACGCCCCAGACCCATGTGCAAAGGAGACAGT ACTGAGTGCCCTCAAAGAGAAGGAGAAGAAAAGGACAGTGGAGGAAGAAGACCAAATATTCCTTGATGGC CAGGAAAATAAAAGAAGGCGCCATGATAGCAGTGGCAGTGGACATTCAGCATTTGAGCCCCTGGTGGCCA ATGGAGTCCCCGCTTCTTTTGTGCCTAAGCCTGGGTCTCTGAAGAGAGGCCTCAATTCTCAGAGCTCAGA TGACCACTTGAATAAGAGATCCCGAAGCTCTTCCATGAGCTCCTTGACAGGCGCTTACGCAAGTGGCATC CCTAGCTCCAGCCGCAATGCCATTACCAGTTCCTACAGCTCCACTCGAGGCATCTCACAGCTCTGGAAGA GAAATGGCCCCAGTTCATCACCCTTCTCTAGCCCAGCCTCCTCCCGCTCCCAGACACCGGAGAGGCCAGC AAAGAAAATAAGAGAAGAGGAGCTGTGTCATCATTCCAGTTCTTCAACTCCATTGGCAGCAGACAGGGAG TCCCAGGGAGAAAAGGCTGCAGATACAACCCCAAGGAAGAAACAAAACTCGAATTCTCAGTCTACACCTG GCAGCTCTGGGCAGCGTAAGCGGAAAGTTCAGCTGCTGCCTTCTCGGCGAGGGGAACAGCTGACCTTGCC TCCACCTCCCCAGCTTGGCTATTCGATCACTGCCGAGGACCTAGACTTAGAGAAGAAGGCTTCATTACAG TGGTTCAACCAGGCCTTGGAGGACAAGAGCGATGCTGCCTCGAACTCTGTCACTGAGACCCCACCTATCA CTCAGCCTTCATTTACCTTTACCCTGCCTGCTGCTGCACCTGCCTCCCCACCCACCTCCCTCCTGGCCCC AAGCACCAACCCACTGTTAGAGAGCTTGAAGAAGATGCAGACTCCCCCGAGCCTGCCACCCTGCCCAGAA TCTGCTGGAGCAGCAACCACTGAGGCCCTCTCACCTCCAAAGACACCCAGCCTCCTACCCCCGCTGGGTT TATCACAGTCAGGGCCGCCAGGGCTGCTCCCCAGCCCCTCCTTTGACTCCAAACCCCCGACCACTTTGCT GGGGCTGATCCCTGCTCCATCCATGGTACCAGCCACTGACACCAAGGCACCTCCAACCCTTCAGGCAGAG ACGGCTACCAAACCCCAAGCCACATCTGCCCCGTCCCCCGCCCCCAAGCAAAGCTTCCTGTTTGGAACAC AGAACACCTCACCTTCCAGCCCTGCCGCCCCTGCTGCATCTTCAGCACCTCCCATGTTCAAGCCCATTTT CACGGCTCCACCCAAGAGTGAGAAGGAAGGCCCCACACCGCCTGGCCCTTCAGTCACAGCCACAGCGCCC TCCAGCTCCTCCCTCCCCACGACCACCAGCACCACAGCCCCGACCTTCCAGCCTGTCTTTAGCAGCATGG GGCCACCTGCATCTGTGCCCTTGCCTGCTCCCTTCTTCAAGCAGACAACTACTCCCGCCACTGCTCCCAC CACAACTGCCCCGCTCTTCACTGGCCTGGCCAGCGCCACCTCTGCTGTGGCTCCCATCACCTCTGCCAGT CCATCCACAGACTCTGCTTCGAAGCCTGCGTTTGGCTTTGGCATAAACAGTGTGAGCAGCAGCAGTGTGA GTACCACGACCAGCACCGCCACTGCCGCCTCACAGCCTTTCCTCTTCGGGGCGCCCCAGGCCTCTGCTGC CAGCTTCACCCCGGCCATGGGCTCCATATTCCAGTTTGGCAAACCTCCTGCCTTGCCCACAACCACCACA GTCACCACCTTCAGCCAGTCCCTGCACACTGCCGTGCCAACGGCCACCAGCAGCAGCGCTGCCGACTTTA GTGGTTTTGGCAGCACCCTCGCCACCTCCGCCCCGGCCACCAGCAGCCAGCCCACTCTGACGTTCAGTAA CACGAGCACCCCCACGTTCAACATTCCCTTTGGCTCAAGCGCCAAGTCCCCGCTCCCATCATATCCGGGA GCCAACCCCCAGCCCGCATTTGGGGCCGCTGAGGGGCAGCCACCGGGGGCCGCCAAGCCGGCCCTTGCCC CCAGCTTTGGCAGCTCTTTCACTTTTGGAAACTCTGCAGCCCCGGCTGCTGCACCCACACCTGCACCTCC GTCCATGATCAAGGTCGTGCCTGCGTACGTGCCTACGCCCATCCATCCTATCTTTGGCGGTGCCACGCAC TCGGCGTTTGGGTTGAAAGCCACGGCTTCGGCCTTCGGCGCTCCCGCCAGCTCACAGCCCGCCTTTGGCG GCTCCACTGCTGTCTTCTTCGGTGCAGCCACCAGCTCCGGCTTTGGAGCCACCACCCAGACCGCCAGCAG CGGGAGCAGCAGCTCGGTGTTTGGCAGCACAACACCATCACCCTTCACGTTTGGGGGTTCGGCAGCCCCC GCTGGCAGTGGGAGCTTTGGGATCAATGTGGCCACCCCAGGCTCCAGCACCACCACCGGAGCTTTCAGCT TTGGAGCAGGACAGAGTGGGAGCACAGCCACCTCCACCCCCTTCGCAGGGGGCTTAGGTCAGAACGCCCT GGGCACCACCGGCCAGAGCACACCGTTTGCCTTCAACGTGAGCAGCACAACTGAGAGCAAACCTGTGTTT GGAGGCACCGCCACCCCCACCTTTGGTCTGAACACCCCTGCGCCTGGAGTGGGCACATCAGGCAGCAGCC TCTCCTTTGGGGCATCCTCAGCACCCGCCCAAGGCTTTGTTGGTGTTGCACCTTTCGGCAACACTTTTGC TCACCAGCAAGAACACAGCCCGAGGAAGGGACCCAATAACCTTTCAAAACGCAAACTGCTGCCTGCGGTG AGGGCCCAGGGTCTTCCACGGAGAGGACAGGCATCTTCCTTTCCCACCAGGAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235113 representing NM_001257190 Red=Cloning site Green=Tags(s) MVCSPVTVRIAPPDRRFSRSAIPEQIISSTLSSPSSNAPDPCAKETVLSALKEKEKKRTVEEEDQIFLDG QENKRRRHDSSGSGHSAFEPLVANGVPASFVPKPGSLKRGLNSQSSDDHLNKRSRSSSMSSLTGAYASGI PSSSRNAITSSYSSTRGISQLWKRNGPSSSPFSSPASSRSQTPERPAKKIREEELCHHSSSSTPLAADRE SQGEKAADTTPRKKQNSNSQSTPGSSGQRKRKVQLLPSRRGEQLTLPPPPQLGYSITAEDLDLEKKASLQ WFNQALEDKSDAASNSVTETPPITQPSFTFTLPAAAPASPPTSLLAPSTNPLLESLKKMQTPPSLPPCPE SAGAATTEALSPPKTPSLLPPLGLSQSGPPGLLPSPSFDSKPPTTLLGLIPAPSMVPATDTKAPPTLQAE TATKPQATSAPSPAPKQSFLFGTQNTSPSSPAAPAASSAPPMFKPIFTAPPKSEKEGPTPPGPSVTATAP SSSSLPTTTSTTAPTFQPVFSSMGPPASVPLPAPFFKQTTTPATAPTTTAPLFTGLASATSAVAPITSAS PSTDSASKPAFGFGINSVSSSSVSTTTSTATAASQPFLFGAPQASAASFTPAMGSIFQFGKPPALPTTTT VTTFSQSLHTAVPTATSSSAADFSGFGSTLATSAPATSSQPTLTFSNTSTPTFNIPFGSSAKSPLPSYPG ANPQPAFGAAEGQPPGAAKPALAPSFGSSFTFGNSAAPAAAPTPAPPSMIKVVPAYVPTPIHPIFGGATH SAFGLKATASAFGAPASSQPAFGGSTAVFFGAATSSGFGATTQTASSGSSSSVFGSTTPSPFTFGGSAAP AGSGSFGINVATPGSSTTTGAFSFGAGQSGSTATSTPFAGGLGQNALGTTGQSTPFAFNVSSTTESKPVF GGTATPTFGLNTPAPGVGTSGSSLSFGASSAPAQGFVGVAPFGNTFAHQQEHSPRKGPNNLSKRKLLPAV RAQGLPRRGQASSFPTRKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001257190
ORF Size	2997 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257190.3
RefSeq Size	7041 bp
RefSeq ORF	3000 bp
Locus ID	9883
UniProt ID	Q96HA1
MW	101.3 kDa
Gene Summary	This gene encodes a transmembrane protein that localizes to the inner nuclear membrane and forms a core component of the nuclear pore complex, which mediates transport to and from the nucleus. The encoded protein may anchor this complex to the nuclear envelope. There are multiple related genes and pseudogenes for this gene on chromosomes 5, 7, 15, and 22. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.