ARHGAP20 (NM_001258415) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RARHOGAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235203 representing NM_001258415 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCCCGGGAGCGGCAGCCGGCGCTGAAGAAGAAAATGAAAACACTAGCAGAAAGGAGGAGGAGCG CTCCATCTCTTATCCTGGATAAAGCCCTACAAAAACGGCCTACTACCAGGGACAGTCCTTCTGCTAGTGT TGACACATGCACATTTCTGTCATCATTAGTGTGCTCCAATAGGACTCTGCTGATTGATGGCCGGGCAGAA CTCAAAAGAGGCCTCCAGAGGCAGGAGCGGCATCTTTTCCTATTCAATGATCTGTTTGTTGTGGCCAAAA TCAAATATAACAATAACTTTAAGATAAAAAATAAAATTAAATTAACTGATATGTGGACAGCAAGCTGTGT GGATGAAGTGGGAGAAGGCAACACCAATGCCATGAAATCCTTTGTTTTGGGCTGGCCCACAGTGAACTTT GTGGCCACTTTCAGTTCTCCAGAACAAAAGGACAAATGGCTCTCTCTCCTTCAGAGATACATCAATCTAG AGAAAGAAAAGGACTACCCGAAGAGCATTCCCCTCAAAATCTTCGCCAAGGACATTGGGAATTGTGCCTA CTCTAAAACTATAACAGTAATGAATTCAGATACAGCGAATGAAGTTATCAACATGTCATTACCAATGCTA GGGATAACTGGCTCTGAGAGAGATTACCAGTTGTGGGTCAATTCTGGCAAAGAAGAGGCTCCATACCCAC TCATTGGGCATGAATATCCATATGGAATTAAAATGAGCCATCTTCGAGACTCTGCACTCCTGACACCGGG ATCAAAGGACTCTACCACCCCTTTCAACCTCCAGGAGCCCTTCCTTATGGAACAGCTCCCCCGAGAGATG CAGTGCCAGTTCATCCTGAAGCCCAGCCGCCTGGCTGCAGCCCAGCAACTGAGTGATTCAGGTCATAAGA CATTTAAAAGGAGAAGATCTATCATAAACTGGGCCTTCTGGCGAGGTTCTAGCACTCACCTGGACAACTT GCCCTCATCGCCAACATCACCTATGCCAGGACAGCTCTTTGGAATTTCTCTGCCAAATATTTGTGAGAAT GACAATCTGCCCAAACCTGTCTTGGATATGCTTTTCTTTCTTAATCAAAAAGGACCTCTCACCAAAGGTA TCTTCAGGCAATCAGCCAATGTGAAATCCTGCAGAGAACTAAAAGAGAAATTGAATTCTGGAGTCGAAGT ACACCTAGACTGTGAATCTATTTTTGTGATAGCATCTGTCTTAAAGGATTTTCTGCGAAATATTCCAGGA AGTATTTTTTCATCAGATCTCTATGATCACTGGGTCTCTGTAATGGATCAAGGAAATGATGAAGAGAAAA TAAATACTGTTCAAAGGCTATTAGACCAGCTTCCGAGAGCCAATGTTGTTCTCCTAAGGTATCTTTTTGG GGTGTTACACAACATTGAGCAACATTCCTCATCCAATCAGATGACTGCATTTAATTTAGCTGTGTGTGTC GCTCCAAGTATTCTTTGGCCTCCTGCTTCCTCCAGCCCAGAACTAGAAAACGAATTTACAAAAAAGGTTT CCCTGCTTATACAATTTCTGATTGAGAATTGCCTTAGGATATTTGGAGAAGAAATCACTTCCCTCTTCAG AGAGGTTTCAGTGAGATGTGACACTAGAGAGAATGCCTCAGATATTTCTTGCTTTCAACTGAATGACTCC TCCTATGACAGCTTGGAAAATGAGCTAAATGAGGATGTTGATGCACCATGCAGTGACTTGGTAAAGAAAC TTGGCCAGGGGAGCAGAAGCATGGACTCTGTCTTAACCCTCAGTGACTATGATCTTGACCAGCCCGAGGT GGAAGGCCTTTTAACCCTAAGCGACTTTGACTTGGCCCATTCTAAAGATGAAGATGTTCAAATGAAACGG CCTCTTGAATCCAAGCCGGTGAACATTTTAGTGTACACAAAGATCCCACTGCGGGATCATGCCAGGGCCC CATCTGCCATGTGCACACCCAGCTACCTGTCCACAGCTGCAGCAAATGCTGCAAAAAGCCTGAGGCGACA CCGGCGTTGCTCAGAGCCCAGCATCGACTATCTGGATTCAAAGCTTTCCTACCTCAGGGAGTTTTATCAG AAAAAGCTACGCAAGTCCAGCTGTGATGCAATTCTTTCTCAAAAAGATGAAGACTATCTGAAGCAGAATC AACCCCTCCAGGAGGAAGGAAAGACATGTTTTAAACAGAGTTTAGTCACAGGCACTGATGTCAGCAAGAA AAATGCCACTACTCAAAACACTAAGAAGAAAAGCTTGTCTGGTAGTGAAGGAAATCACGTGAAACTTTTC CCTAAGTCTAAGCCAGTGGCCATTTCTGTGGCATCTTATAGTCCTATGTCCTCACAGGATCATTCCAAGA ACCAGCCCTTTGATGTGAATACATCTGGATACTCCCCACCACACACAGCAGATGCCCTCAAGGGTCCAAG GACACATCGGCGCTGCTCAGAGCCCAACATAGAAGACCAGAACCGCAAGCTGACCTATCTCAGGGGAATT TATTCAAAGAAACAACATAAAACCAGCTGTGAAGCTGGTCTCTTGCATGGAGAGGAGGATTATCTCAAAC GGCATAAGTCTTTGCAAATGGAGGGGCAGAAGCTCATTAATCAGAGTTTAGTCATGGGGATTGAGGTGGG CAAGAGTAGTGCCACAAACCAAAACACTGAGAAGGTTTTACCCCCAAGATTAAACCTTTGCCCAAGGACC AGCTATTCCAGCTTATCCTCCCCAGGCACTTCCCCATCCGGCTCATCAGTAAGCTCCCAAGACAGTGCTT TTTCTCAGATTTCTGAACACTCTGTGTTTACACCCACTGAGACTTCCTCTCCAATAGATTGCACTTTTCA GGCTCAGAGAAAACGGGAAGACCTTTCTCCTGACTTTAGCAATGCCAGCCATGTTTCCGGAATGCCCGGT CCCTCATCAGGGCAGGCTTGCAGCCGCCCAGCCTATACAAAGAAGGACACCATGGAGTGGCATTCACAAA TGCATTCTGTAACTCTTCATCCCAGCACATGGTTGAGAAATGGTGTGGCCAGTTTGAAAAACTGGTCCCT CAAAAAGAAAGCAAAGGCAGCCAGACCAGAGGAAGAGAAAATAGCTTCTCCAAAAGGACCCTTAGAGCCA CCCCCACATGCTTCTGGTGTTCCAGAAGCCAACTCACTGCAAGAGGAACAAAAAGACTTGCCCTTAAGGG CAGCTGAAGGACTGTCCCCTGTGCAGTCAGCCCAAAGGTGTAGTTCTTCTCCCTTCCAGGACTCAGAGAG ACACTGTAGCTCTCCATTCAGCCTGGTGGAGAGCAGACTTAAGCTGTGCATGAAGTCACATGAGGAAATA GAGCCTGGTAGTCAGAGCTCTTCTGGTTCTCTGCCTTGGGAAAGAGCCTCAGCCAGCTCTTGGACTCTAG AGGATGCGACCAGCCCAGACTCAGGGCCTACAGTGGTCTGCGACATTGAGGACAGGTATTTAACCAAAGA CATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235203 representing NM_001258415 Red=Cloning site Green=Tags(s) MSARERQPALKKKMKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAE LKRGLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNF VATFSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPML GITGSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREM QCQFILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICEN DNLPKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPG SIFSSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCV APSILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDS SYDSLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKR PLESKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQ KKLRKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLF PKSKPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGI YSKKQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRT SYSSLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPG PSSGQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEP PPHASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEI EPGSQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258415
ORF Size	3504 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258415.2
RefSeq Size	6199 bp
RefSeq ORF	3507 bp
Locus ID	57569
UniProt ID	Q9P2F6
MW	130.9 kDa
Gene Summary	The protein encoded by this gene is an activator of RHO-type GTPases, transducing a signal from RAP1 to RHO and impacting neurite outgrowth. [provided by RefSeq, Sep 2016]

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