Liprin alpha 2 (PPFIA2) (NM_001220475) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235233 representing NM_001220475 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGTGAAGTGATGCCCACGATTAATGAGGACACCCCAATGAGCCAAAGGGGGTCCCAAAGCAGTG GCTCGGACTCAGACTCCCATTTTGAGCAGCTGATGGTGAATATGCTAGATGAAAGGGATCGTCTTCTAGA CACCCTTCGGGAGACCCAGGAAAGCCTCTCACTTGCCCAGCAAAGACTTCAGGATGTCATCTATGACCGA GACTCACTCCAGAGACAGCTCAATTCAGCCCTGCCACAGGATATCGAATCCCTAACAGGAGGGCTGGCTG GTTCTAAGGGGGCTGATCCACCGGAATTTGCTGCACTGACAAAAGAATTAAATGCCTGCAGGGAACAACT TCTAGAAAAGGAAGAAGAAATCTCTGAACTTAAAGCTGAAAGAAACAACACAAGACTATTACTGGAGCAT TTGGAGTGCCTTGTGTCACGACATGAAAGATCACTAAGAATGACGGTGGTAAAACGGCAAGCCCAGTCTC CCTCAGGAGTATCCAGTGAAGTTGAAGTTCTCAAGGCACTGAAATCTTTGTTTGAGCACCACAAGGCCTT GGATGAAAAGGTAAGGGAGCGACTGAGGGTTTCTTTAGAAAGAGTCTCTGCACTGGAAGAAGAACTAGCT GCTGCTAATCAGGAGATTGTTGCCTTGCGTGAACAAAATGTTCATATACAAAGAAAAATGGCATCAAGCG AGGGATCCACAGAGTCAGAACATCTTGAAGGGATGGAACCTGGACAGAAAGTCCATGAGAAGCGTTTGTC CAATGGTTCTATAGACTCAACCGATGAAACTAGTCAAATAGTTGAACTACAAGAATTGCTTGAAAAGCAA AACTATGAAATGGCCCAGATGAAAGAACGTTTAGCAGCCCTTTCTTCCCGAGTGGGAGAGGTGGAACAGG AAGCAGAGACAGCAAGAAAGGATCTCATTAAAACAGAAGAAATGAACACCAAGTATCAAAGGGACATTAG GGAGGCCATGGCACAAAAGGAAGATATGGAAGAAAGAATTACAACCCTTGAAAAGCGTTACCTCAGTGCT CAGAGAGAATCTACCTCCATACATGACATGAATGATAAACTAGAAAATGAGTTAGCAAATAAAGAAGCTA TCCTGCGGCAGATGGAAGAGAAAAACAGACAGTTACAAGAACGTCTTGAGCTAGCTGAACAAAAGTTGCA GCAGACCATGAGAAAGGCTGAAACCTTGCCTGAAGTAGAGGCTGAACTGGCTCAGAGAATTGCAGCCCTA ACCAAGGCTGAAGAGAGACATGGAAATATTGAAGAACGTATGAGACATTTAGAGGGTCAACTTGAAGAGA AGAATCAAGAACTTCAAAGAGCTAGGCAAAGAGAGAAAATGAATGAGGAGCATAACAAGAGATTATCGGA TACGGTTGATAGACTTCTGACTGAATCCAATGAACGCCTACAACTACACTTAAAGGAAAGAATGGCTGCT CTAGAAGAAAAGAATGTTTTAATTCAAGAATCAGAAACTTTCAGAAAGAATCTTGAAGAATCTTTACATG ATAAGGAAAGATTAGCAGAAGAAATTGAAAAGCTGAGATCTGAACTTGACCAATTGAAAATGAGAACTGG CTCTTTAATTGAACCCACAATACCAAGAACTCATCTAGACACCTCAGCTGAGTTGCGGTACTCAGTGGGA TCCCTAGTGGACAGCCAGTCTGATTACAGAACAACTAAAGTAATAAGAAGACCAAGGAGAGGCCGCATGG GTGTGCGAAGAGATGAGCCAAAGGTGAAATCTCTTGGGGATCACGAGTGGAATAGAACTCAACAGATTGG AGTACTAAGCAGCCACCCTTTTGAAAGTGACACTGAAATGTCTGATATTGATGATGATGACAGAGAAACA ATTTTTAGCTCAATGGATCTTCTCTCTCCAAGTGGTCATTCCGATGCCCAGACGCTAGCCATGATGCTTC AGGAACAATTGGATGCCATCAACAAAGAAATCAGGCTAATTCAGGAAGAAAAAGAATCTACAGAGTTGCG TGCTGAAGAAATTGAAAATAGAGTGGCTAGTGTGAGCCTCGAAGGCCTGAATTTGGCAAGGGTCCACCCA GGTACCTCCATTACTGCCTCTGTTACAGCTTCATCGCTGGCCAGTTCATCTCCCCCCAGTGGACACTCAA CTCCAAAGCTCACCCCTCGAAGCCCTGCCAGGGAAATGGATCGGATGGGAGTCATGACACTGCCAAGTGA TCTGAGGAAACATCGGAGAAAGATTGCAGTTGTGGAAGAAGATGGTCGAGAGGACAAAGCAACAATTAAA TGTGAAACTTCTCCTCCTCCTACCCCTAGAGCCCTCAGAATGACTCACACTCTCCCTTCTTCCTACCACA ATGATGCTCGAAGTAGTTTATCTGTCTCTCTTGAGCCAGAAAGCCTCGGGCTTGGTAGTGCCAACAGCAG CCAAGACTCTCTTCACAAAGCCCCCAAGAAGAAAGGAATCAAGTCTTCAATAGGACGTTTGTTTGGTAAA AAAGAAAAAGCTCGACTTGGGCAGCTCCGAGGCTTTATGGAGACTGAAGCTGCAGCTCAGGAGTCCCTGG GGTTAGGCAAACTCGGAACTCAAGCTGAGAAGGATCGAAGACTAAAGAAAAAGCATGAACTTCTTGAAGA AGCTCGGAGAAAGGGATTACCTTTTGCCCAGTGGGATGGGCCAACTGTGGTCGCATGGCTAGAGCTTTGG TTGGGAATGCCTGCGTGGTACGTGGCAGCCTGCCGAGCCAACGTGAAGAGTGGTGCCATCATGTCTGCTT TATCTGACACTGAGATCCAGAGAGAAATTGGAATCAGCAATCCACTGCATCGCTTAAAACTTCGATTAGC AATCCAGGAGATGGTTTCCCTAACAAGTCCTTCAGCTCCTCCAACATCTCGAACTAAAGAATCTGAGGAA GGAAGCTGGGCCCAGTGTCCGGTTTTTCTACAGACCCTGGCTTATGGAGATATGAATCATGAGTGGATTG GAAATGAATGGCTTCCCAGCTTGGGGTTACCTCAGTACAGAAGTTACTTTATGGAATGCTTGGTAGATGC AAGAATGTTAGATCACCTAACAAAAAAAGATCTCCGTGTCCATTTAAAAATGGTGGATAGTTTCCATCGA ACAAGTTTACAATATGGAATTATGTGCTTAAAGAGGTTGAATTATGACAGAAAAGAACTAGAAAGAAGAC GGGAAGCAAGCCAACATGAAATAAAAGACGTGTTGGTGTGGAGCAATGACCGAGTTATTCGCTGGATACA AGCAATTGGACTTCGAGAATATGCAAATAATATACTTGAGAGCGGTGTGCATGGCTCACTTATAGCCCTG GATGAAAACTTTGACTACAGCAGCTTAGCTTTATTATTACAGATTCCAACACAGAACACCCAGGCAAGGC AGATTCTTGAAAGAGAATACAATAACCTCTTGGCCCTGGGAACTGAAAGGCGACTGGATGAAAGTGATGA CAAGAACTTCAGACGTGGATCAACCTGGAGAAGGCAGTTTCCTCCTCGTGAAGTACATGGAATCAGCATG ATGCCTGGGTCCTCAGAAACATTACCAGCTGGATTTAGGTTAACCACAACCTCTGGGCAGTCAAGAAAAA TGACAACAGATGTTGCTTCATCAAGACTGCAGAGGTTAGACAACTCCACTGTTCGCACATACTCATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235233 representing NM_001220475 Red=Cloning site Green=Tags(s) MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLSLAQQRLQDVIYDR DSLQRQLNSALPQDIESLTGGLAGSKGADPPEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEH LECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELA AANQEIVALREQNVHIQRKMASSEGSTESEHLEGMEPGQKVHEKRLSNGSIDSTDETSQIVELQELLEKQ NYEMAQMKERLAALSSRVGEVEQEAETARKDLIKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSA QRESTSIHDMNDKLENELANKEAILRQMEEKNRQLQERLELAEQKLQQTMRKAETLPEVEAELAQRIAAL TKAEERHGNIEERMRHLEGQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAA LEEKNVLIQESETFRKNLEESLHDKERLAEEIEKLRSELDQLKMRTGSLIEPTIPRTHLDTSAELRYSVG SLVDSQSDYRTTKVIRRPRRGRMGVRRDEPKVKSLGDHEWNRTQQIGVLSSHPFESDTEMSDIDDDDRET IFSSMDLLSPSGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARVHP GTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIK CETSPPPTPRALRMTHTLPSSYHNDARSSLSVSLEPESLGLGSANSSQDSLHKAPKKKGIKSSIGRLFGK KEKARLGQLRGFMETEAAAQESLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELW LGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTKESEE GSWAQCPVFLQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHR TSLQYGIMCLKRLNYDRKELERRREASQHEIKDVLVWSNDRVIRWIQAIGLREYANNILESGVHGSLIAL DENFDYSSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGSTWRRQFPPREVHGISM MPGSSETLPAGFRLTTTSGQSRKMTTDVASSRLQRLDNSTVRTYSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001220475
ORF Size	3708 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001220475.2, NP_001207404.1
RefSeq Size	5540 bp
RefSeq ORF	3711 bp
Locus ID	8499
UniProt ID	O75334
Protein Families	Druggable Genome
MW	141.5 kDa
Gene Summary	The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein has been shown to bind the calcium/calmodulin-dependent serine protein kinase (MAGUK family) protein (also known as CASK) and proposed to regulate higher-order brain functions in mammals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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