USP19 (NM_001199160) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ZMYND9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235299 representing NM_001199160 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCCAGGACTGGAGGACACCACTAGTAAGA AGAAGCAGAAGGATCGAGCAAACCAGGAGAGCAAGGATGGAGATCCTAGGAAAGAGACAGGGTCTCGATA TGTTGCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGATCCTTCTGCCTCAGCCTCCCATGCAGCTGGG ATCACAGGCTCACGCCACCGTACCCGGCTGTTCTTTCCTTCATCGTCAGGGTCAGCATCCACTCCTCAAG AGGAGCAGACCAAAGAGGGAGCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGAGTTGTT GCTCGATTGGAGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTCGTGTGGGAGTAGGTCCCCTGCAGCTG GAGGATGTAGATGCTGCTTTCACAGATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAGCAGTGGGGTG GTGTCTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGCAAGGGCAGTCTCCTGCACCT GACACTGCCCAAAAAGGTGCCTATGCTCACGTGGCCCTCCCTCCTGAAGAAACCTCTAGGGACCCAGGAG CTGGTGCCGGGGCTGCGGTGCCAGGAGAATGGGCAGGAACTGTCTCCCATTGCCCTGGAGCCAGGCCCTG AGCCCCACCGGGCTAAGCAGGAGGCCCGGAACCAGAAGCGGGCCCAGGGCCGTGGTGAGGTAGGCGCAGG GGCTGGCCCCGGGGCCCAGGCAGGGCCCAGCGCCAAGAGGGCTGTGCATCTCTGCAGAGGGCCAGAGGGG GACGGGTCCAGGGATGACCCTGGACCCCGGGGTGATGCCCCACCCTTCGTGGCTGACCCAGCCACCCAGG TTGAGGCTGATGAACAGCTTTGCATACCACCGCTGAACTCCCAAACCTGCCTCCTGGGCTCAGAGGAGAA TTTAGCCCCTTTGGCAGGAGAGAAAGCAGTGCCTCCCGGGAATGACCCAGTCTCTCCAGCCATGGTCCGG AGCAGAAACCCTGGGAAAGATGACTGTGCCAAGGAGGAGATGGCAGTGGCAGCAGATGCTGCAACCTTGG TGGATGAGCCCGAGTCGATGGTGAACCTGGCGTTTGTCAAGAATGACTCGTATGAGAAGGGCCCGGATTC AGTGGTGGTGCACGTGTACGTGAAGGAGATCTGCAGGGACACCTCAAGAGTACTTTTCCGTGAGCAGGAC TTCACGCTCATCTTCCAGACCAGGGATGGAAACTTCCTGAGGCTGCACCCGGGCTGTGGGCCCCACACCA CCTTCCGTTGGCAGGTGAAGCTCAGGAATCTGATTGAGCCAGAGCAGTGCACCTTCTGTTTCACGGCTTC TCGCATCGACATCTGCCTTCGTAAGAGGCAGAGTCAGCGCTGGGGGGGCCTGGAGGCCCCGGCTGCACGA GTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGTCCAACCCCTCTGGATTCAACCCCACCAGGAGGTGCTC CCCACCCCCTGACAGGCCAGGAGGAGGCCCGGGCTGTGGAGAAGGATAAATCCAAGGCACGATCTGAGGA CACAGGGCTAGACAGTGTGGCAACCCGCACACCCATGGAGCATGTAACCCCAAAGCCAGAGACACACCTG GCCTCGCCCAAGCCTACATGCATGGTGCCTCCCATGCCCCACAGCCCAGTTAGTGGAGACAGCGTGGAGG AGGAGGAAGAGGAAGAGAAGAAGGTGTGTCTGCCAGGCTTCACTGGCCTTGTCAATTTAGGCAACACCTG CTTCATGAACAGCGTCATTCAGTCTCTGTCCAACACTCGGGAACTCCGGGACTTCTTCCATGACCGCTCC TTTGAGGCTGAGATCAACTACAACAACCCACTAGGGACTGGTGGGCGTCTGGCCATTGGCTTTGCCGTGC TGCTTCGGGCGCTGTGGAAGGGCACCCACCATGCCTTCCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAG TAAGGCCAGCCAGTTCACAGGCTATGCACAGCATGATGCCCAGGAGTTCATGGCTTTCCTGCTGGATGGG CTGCACGAGGACCTGAATCGCATTCAGAACAAGCCCTACACAGAGACCGTGGATTCAGATGGGCGGCCCG ATGAGGTGGTAGCTGAGGAAGCATGGCAGCGGCACAAGATGAGGAATGACTCTTTCATCGTGGACCTATT TCAGGGGCAGTACAAGTCGAAGCTGGTGTGCCCTGTGTGTGCCAAGGTCTCCATCACTTTTGACCCGTTT CTTTATCTGCCGGTGCCCTTGCCACAAAAGCAAAAGGTTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCC ACAGCAAGCCCATCAAGTTCCTGGTGAGCGTCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGACTC CCTCTCTCAGAGTGTTCATGTGAAGCCTGAGAACCTGCGTTTGGCGGAGGTAATTAAGAATCGTTTTCAT CGTGTGTTCCTACCCTCCCACTCACTGGACACTGTGTCCCCATCTGATACGCTCCTCTGCTTTGAGCTGC TATCCTCAGAGTTGGCTAAGGAGCGGGTAGTGGTGCTAGAGGTGCAACAGCGCCCCCAGGTGCCCAGCGT CCCCATCTCCAAGTGTGCAGCCTGCCAGCGGAAGCAACAGTCGGAGGATGAAAAGCTGAAGCGCTGTACC CGGTGCTACCGTGTGGGCTACTGCAACCAGCTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCCTCT GCCGACCTGAGAACATTGGCTACCCCTTCCTGGTCAGTGTACCTGCCTCACGCCTCACTTATGCCCGCCT CGCTCAGTTGCTAGAGGGCTATGCCCGGTACTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGCCGC ATGGCCTTGGAGTCTCAGAGCCCTGGCTGCACCACACTGCTCTCCACAGGTTCCCTGGAGGCTGGGGACA GCGAGAGAGACCCCATTCAGCCACCTGAGCTCCAGCTGGTGACCCCTATGGCTGAGGGGGACACAGGGCT TCCCCGGGTGTGGGCAGCCCCTGACCGGGGTCCTGTGCCCAGCACCAGTGGAATTTCTTCTGAGATGCTG GCCAGTGGGCCCATTGAGGTTGGCTCCTTGCCAGCTGGCGAGAGGGTGTCCCGACCCGAAGCTGCTGTGC CTGGGTACCAGCATCCAAGTGAAGCTATGAATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTC ATCCAACCGAGAGCAGCGGCTAGAGGACAAAGGAGACACCCCACTGGAGCTGGGTGACGACTGTAGCCTG GCTCTCGTCTGGCGGAACAATGAGCGCTTGCAGGAGTTTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTG CTGAGGATCCAGGCTCTGCCGGTGAGGCTGCCCGGGCCGGCCACTTCACCCTGGACCAGTGCCTCAACCT CTTCACACGGCCTGAGGTGCTGGCACCCGAGGAGGCCTGGTACTGCCCACAGTGCAAACAGCACCGTGAG GCCTCCAAGCAGCTGTTGCTATGGCGCCTGCCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTC GTAGTTTTATCTGGCGTGACAAGATCAATGACTTGGTGGAGTTCCCTGTTAGGAACCTGGACCTGAGCAA GTTCTGCATTGGTCAGAAAGAGGAGCAGCTGCCCAGCTACGATCTATATGCTGTCATCAACCACTATGGA GGCATGATTGGTGGCCACTACACTGCCTGTGCACGCCTGCCCAATGATCGTAGCAGTCAGCGCAGTGACG TGGGCTGGCGCTTGTTTGATGACAGCACAGTGACAACGGTAGACGAGAGCCAGGTTGTGACGCGTTATGC CTATGTACTCTTCTACCGCCGGCGGAACTCTCCTGTGGAGAGGCCCCCCAGGGCAGGTCACTCTGAGCAC CACCCAGACCTAGGCCCTGCAGCTGAGGCTGCTGCCAGCCAGGCTTCCCGGATTTGGCAGGAGCTGGAGG CTGAGGAGGAGCCGGTGCCTGAGGGGTCTGGGCCCCTGGGTCCCTGGGGGCCCCAAGACTGGGTGGGCCC CCTACCACGTGGCCCTACCACACCAGATGAGGGCTGCCTCCGGTACTTTGTCCTGGGCACCGTGGCGGCT TTGGTGGCCCTCGTGCTCAACGTGTTCTATCCTCTGGTATCCCAGAGTCGCTGGAGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235299 representing NM_001199160 Red=Cloning site Green=Tags(s) MSGGASATGPRRGPPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAGLEPLASGDPSASASHAAG ITGSRHRTRLFFPSSSGSASTPQEEQTKEGACEDPHDLLATPTPELLLDWRQSAEEVIVKLRVGVGPLQL EDVDAAFTDTDCVVRFAGGQQWGGVFYAEIKSSCAKVQTRKGSLLHLTLPKKVPMLTWPSLLKKPLGTQE LVPGLRCQENGQELSPIALEPGPEPHRAKQEARNQKRAQGRGEVGAGAGPGAQAGPSAKRAVHLCRGPEG DGSRDDPGPRGDAPPFVADPATQVEADEQLCIPPLNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVR SRNPGKDDCAKEEMAVAADAATLVDEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEICRDTSRVLFREQD FTLIFQTRDGNFLRLHPGCGPHTTFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPAAR VGGAKVAVPTGPTPLDSTPPGGAPHPLTGQEEARAVEKDKSKARSEDTGLDSVATRTPMEHVTPKPETHL ASPKPTCMVPPMPHSPVSGDSVEEEEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSNTRELRDFFHDRS FEAEINYNNPLGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDG LHEDLNRIQNKPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPVCAKVSITFDPF LYLPVPLPQKQKVLPVFYFAREPHSKPIKFLVSVSKENSTASEVLDSLSQSVHVKPENLRLAEVIKNRFH RVFLPSHSLDTVSPSDTLLCFELLSSELAKERVVVLEVQQRPQVPSVPISKCAACQRKQQSEDEKLKRCT RCYRVGYCNQLCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYSVSVFQPPFQPGR MALESQSPGCTTLLSTGSLEAGDSERDPIQPPELQLVTPMAEGDTGLPRVWAAPDRGPVPSTSGISSEML ASGPIEVGSLPAGERVSRPEAAVPGYQHPSEAMNAHTPQFFIYKIDSSNREQRLEDKGDTPLELGDDCSL ALVWRNNERLQEFVLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEEAWYCPQCKQHRE ASKQLLLWRLPNVLIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPSYDLYAVINHYG GMIGGHYTACARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSPVERPPRAGHSEH HPDLGPAAEAAASQASRIWQELEAEEEPVPEGSGPLGPWGPQDWVGPLPRGPTTPDEGCLRYFVLGTVAA LVALVLNVFYPLVSQSRWR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199160
ORF Size	4257 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199160.1, NP_001186089.1
RefSeq Size	4878 bp
RefSeq ORF	4260 bp
Locus ID	10869
UniProt ID	O94966
Protein Families	Druggable Genome, Protease, Transmembrane
MW	156.5 kDa
Gene Summary	Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This protein is a ubiquitin protein ligase and plays a role in muscle wasting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017]

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