BATF2 (NM_001300807) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SARI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC236930 representing NM_001300807 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCTGTGGGCAAGAGAATAGGTGGTTTGGGGGACACACGGGTTGGAGGCCCGTGCATATCCCAGC AGCACGAGTCTCTGGAAAAAGACAACCTCGCCCTGCGGAAGGAGATCCAGTCCCTGCAGGCCGAGCTGGC GTGGTGGAGCCGGACCCTGCACGTGCATGAGCGCCTGTGCCCCATGGATTGTGCCTCCTGCTCAGCTCCA GGGCTCCTGGGCTGCTGGGACCAGGCTGAGGGGCTCCTGGGCCCTGGCCCACAGGGACAACATGGCTGCC GGGAGCAGCTGGAGCTGTTCCAGACCCCGGGTTCCTGTTACCCAGCTCAGCCGCTCTCTCCAGGTCCACA GCCTCATGATTCTCCCAGCCTCCTCCAGTGCCCCCTGCCCTCACTGTCCCTTGGCCCCGCTGTGGTTGCT GAACCTCCTGTCCAGCTGTCCCCCAGCCCTCTCCTGTTTGCCTCGCACACTGGTTCCAGCCTGCAGGGGT CTTCCTCTAAGCTCAGTGCCCTCCAGCCCAGCCTCACGGCCCAAACTGCCCCTCCACAGCCCCTCGAGCT GGAGCATCCCACCAGAGGGAAGCTGGGGTCCTCTCCCGACAACCCTTCCTCTGCCCTGGGGCTTGCACGT CTGCAGAGCAGGGAGCACAAACCTGCTCTCTCAGCAGCCACTTGGCAAGGGCTGGTTGTGGATCCCAGCC CTCACCCTCTCCTGGCCTTTCCTCTGCTCTCCTCTGCTCAAGTCCACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC236930 representing NM_001300807 Red=Cloning site Green=Tags(s) MAPVGKRIGGLGDTRVGGPCISQQHESLEKDNLALRKEIQSLQAELAWWSRTLHVHERLCPMDCASCSAP GLLGCWDQAEGLLGPGPQGQHGCREQLELFQTPGSCYPAQPLSPGPQPHDSPSLLQCPLPSLSLGPAVVA EPPVQLSPSPLLFASHTGSSLQGSSSKLSALQPSLTAQTAPPQPLELEHPTRGKLGSSPDNPSSALGLAR LQSREHKPALSAATWQGLVVDPSPHPLLAFPLLSSAQVHF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300807
ORF Size	750 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300807.1, NP_001287736.1
RefSeq Size	2074 bp
RefSeq ORF	753 bp
Locus ID	116071
UniProt ID	Q8N1L9
Protein Families	Transcription Factors
MW	26.8 kDa
Gene Summary	AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system. Following infection, participates in the differentiation of CD8(+) thymic conventional dendritic cells in the immune system. Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes (By similarity). Selectively suppresses CCN1 transcription and hence blocks the downstream cell proliferation signals produced by CCN1 and inhibits CCN1-induced anchorage-independent growth and invasion in several cancer types, such as breast cancer, malignant glioma and metastatic melanoma. Possibly acts by interfering with AP-1 binding to CCN1 promoter.[UniProtKB/Swiss-Prot Function]

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