METTL9 (NM_001288660) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CGI-81; DREV; DREV1; PAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237110 representing NM_001288660 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGAAAGACCACGAGCTGGGATACCAAGTTCTATGAAAAGTGGTATGTGTGCAACAGAGAGAAAT TATGCGAATCACTCCAGGCTGTCTTTGTTCAGAGTTACCTTGATCAAGGAACACAGATCTTCTTAAACAA CAGCATTGAGAAATCGGGCTGGCTATTTATCCAATTATATCATTCTTTTGTGTCATCTGTTTTTAGCCTG TTTATGTCTAGAACATCTATCAATGGGTTGCTAGGAAGAGGCTCAATGTTTGTGTTTTCACCAGATCAGT TTCAGAGACTGCTTAAAATTAATCCAGACTGGAAAACCCACAGACTTCTTGATTTAGGTGCTGGAGATGG AGAAGTCACAAAAATCATGAGCCCTCATTTTGAAGAAATCTATGCCACTGAGCTTTCTGAAACTATGATA TGGCAGCTTCAGAAAAAGAAATACAGAGTCCTTGGTATAAATGAATGGCAGAATACGGGGTTCCAGTATG ATGTCATCAGCTGCCTGAACTTGCTGGACCGCTGTGATCAGCCCCTGACTTTGTTAAAAGATATCAGAAG TGTCTTGGAGCCAACTAGAGGCAGGGTCATCCTTGCCCTTGTCCTCCCCTTTCATCCCTATGTGGAAAAC GGTGGCAAGTGGGAGAAACCATCAGAAATTTTGGAAATCAAAGGACAGAACTGGGAAGAACAAGTGAATA GTCTGCCTGAAGTTTTCAGAAAAGCTGGTTTTGTTATCGAAGCTTTCACCAGACTACCATACCTGTGTGA AGGCGACATGTATAATGACTACTACGTTCTGGATGACGCTGTCTTTGTTCTCAAACCAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237110 representing NM_001288660 Red=Cloning site Green=Tags(s) MDRKTTSWDTKFYEKWYVCNREKLCESLQAVFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSL FMSRTSINGLLGRGSMFVFSPDQFQRLLKINPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMI WQLQKKKYRVLGINEWQNTGFQYDVISCLNLLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPYVEN GGKWEKPSEILEIKGQNWEEQVNSLPEVFRKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLKPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288660
ORF Size	831 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288660.1, NP_001275589.1
RefSeq Size	2921 bp
RefSeq ORF	834 bp
Locus ID	51108
UniProt ID	Q9H1A3
MW	32.8 kDa
Gene Summary	Protein-histidine N-methyltransferase that specifically catalyzes 1-methylhistidine (pros-methylhistidine) methylation of target proteins (PubMed:33563959). Mediates methylation of proteins with a His-x-His (HxH) motif (where 'x' is preferably a small amino acid) (PubMed:33563959). Catalyzes methylation of target proteins such as S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12; 1-methylhistidine modification may affect the binding of zinc and other metals to its target proteins (PubMed:33563959). Constitutes the main methyltransferase for the 1-methylhistidine modification in cell (PubMed:33563959).[UniProtKB/Swiss-Prot Function]

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