ZDHHC20 (NM_001286638) Human Tagged ORF Clone
CAT#: RC237245
- TrueORF®
ZDHHC20 (myc-DDK-tagged) - Human zinc finger, DHHC-type containing 20 (ZDHHC20), transcript variant 2
ORF Plasmid: tGFP
"NM_001286638" in other vectors (2)
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CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4933421L13Rik; DHHC-20; DHHC20 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237245 representing NM_001286638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGTATGGTCCTATTGGATGACAATTTTCACATCTCCCGCTTCCCCCTCCAAAGAGTTCTACTTGT CCAATTCTGAAAAGGAACGTTATGAAAAAGAATTCAGCCAAGAAAGACAACAAGAAATTTTGAGAAGAGC AGCAAGAGCTTTACCTATCTATACCACATCAGCTTCAAAAACTATCAGATATTGTGAAAAATGTCAGCTG ATTAAACCTGATCGGGCGCATCACTGCTCAGCCTGTGACTCATGTATTCTTAAGATGGATCATCACTGTC CTTGGGTGAATAACTGTGTGGGATTTTCTAATTACAAATTCTTCCTGCTGTTTTTATTGTATTCCCTATT ATATTGCCTTTTCGTGGCTGCAACAGTTTTAGAGTACTTTATAAAATTTTGGACGAATGAACTGACAGAT ACACGTGCAAAATTCCACGTACTTTTTCTTTTCTTTGTGTCTGCAATGTTCTTCATCAGCGTCCTCTCAC TTTTCAGCTACCACTGCTGGCTAGTTGGAAAAAATAGAACAACAATAGAATCATTCCGCGCACCCACGTT TTCATACGGACCTGATGGAAATGGTTTCTCTCTTGGATGCAGTAAAAATTGGAGACAAGTCTTTGGTGAT GAAAAGAAATATTGGCTACTTCCAATATTTTCAAGCTTGGGTGATGGTTGCAGTTTTCCAACTCGCCTTG TGGGGATGGATCCAGAACAAGCTTCTGTTACAAACCAGAATGAGTATGCCAGAAGTAGTGGCTCAAATCA ACCTTTTCCTATCAAACCACTTAGTGAATCAAAAAACCGCTTGTTGGACAGTGAATCTCAGTGGCTGGAG AATGGAGCTGAAGAAGGCATCGTCAAATCAGGTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237245 representing NM_001286638
Red=Cloning site Green=Tags(s) MFVWSYWMTIFTSPASPSKEFYLSNSEKERYEKEFSQERQQEILRRAARALPIYTTSASKTIRYCEKCQL IKPDRAHHCSACDSCILKMDHHCPWVNNCVGFSNYKFFLLFLLYSLLYCLFVAATVLEYFIKFWTNELTD TRAKFHVLFLFFVSAMFFISVLSLFSYHCWLVGKNRTTIESFRAPTFSYGPDGNGFSLGCSKNWRQVFGD EKKYWLLPIFSSLGDGCSFPTRLVGMDPEQASVTNQNEYARSSGSNQPFPIKPLSESKNRLLDSESQWLE NGAEEGIVKSGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001286638 |
ORF Size | 876 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286638.1, NP_001273567.1 |
RefSeq Size | 5333 bp |
RefSeq ORF | 879 bp |
Locus ID | 253832 |
UniProt ID | Q5W0Z9 |
Protein Families | Transmembrane |
MW | 34.2 kDa |
Gene Summary | Catalyzes palmitoylation of Cys residues on target proteins (PubMed:27153536, PubMed:29326245). Catalyzes palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, and modulates the duration of EGFR signaling by modulating palmitoylation-dependent EGFR internalization and degradation (PubMed:27153536). Has a preference for acyl-CoA with C16 fatty acid chains (PubMed:29326245). Can also utilize acyl-CoA with C14 and C18 fatty acid chains (PubMed:29326245).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The palmitoyltransferase ZDHHC20 enhances interferon-induced transmembrane protein 3 (IFITM3) palmitoylation and antiviral activity
,null,
The Journal of Biological Chemistry
,PubMed ID 29079573
[ZDHHC20]
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