VPS36 (NM_001282169) Human Tagged ORF Clone
CAT#: RC237476
- TrueORF®
VPS36 (myc-DDK-tagged) - Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36), transcript variant 3
ORF Plasmid: tGFP
"NM_001282169" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C13orf9; CGI-145; EAP45 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237476 representing NM_001282169
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATTCTCCTTTCCCAAATTGTGTTCATTGAAGAACAGGCGGCTGGAATTGGGAAGAGTGCCAAAA TAGTGGTTCATCTTCACCCAGCTCCTCCTAACAAAGAACCTGGCCCATTCCAGAGTAGTAAGAACTCCTA CATCAAACTCTCCTTCAAAGAACATGGCCAGATTGAGTTTTACAGGCGTTTATCAGAGGAAATGACACAA AGAAGATGGGAGAATATGCCAGTTTCCCAGTCATTACAAACAAATAGAGGACCCCAGCCAGGAAGAATAA GGGCTGTAGGAATTGTAGGTATTGAAAGGAAACTGGAAGAAAAAAGAAAAGAAACTGACAAAAACATTTC TGAGGCCTTTGAAGACCTCAGCAAACTAATGATCAAGGCTAAGGAAATGGTGGAATTATCAAAATCAATT GCTAATAAAATTAAAGACAAACAAGGTGACATCACAGAAGATGAGACCATCAGGTTTAAATCCTACTTGC TGAGCATGGGAATAGCTAACCCAGTTACCAGAGAAACCTACGGCTCAGGCACACAGTACCACATGCAGCT GGCCAAACAACTGGCTGGAATATTGCAGGTGCCTTTAGAGGAACGAGGGGGAATAATGTCACTCACGGAG GTGTACTGCTTAGTAAACCGAGCTCGAGGAATGGAATTGCTCTCACCAGAAGATTTAGTGAATGCGTGCA AGATGCTGGAAGCACTGAAATTACCTCTCAGGCTCCGTGTGTTTGACAGTGGCGTCATGGTAATTGAGCT TCAGTCTCACAAGGAAGAGGAAATGGTGGCCTCGGCCCTGGAGACAGTTTCAGAAAAGGGATCCCTAACA TCAGAAGAGTTTGCTAAGCTTGTGGGAATGTCTGTCCTCCTAGCCAAAGAAAGGTTGCTGCTTGCAGAGA AGATGGGCCATCTTTGCCGTGATGACTCAGTGGAAGGCCTGCGTTTTTACCCAAATTTATTTATGACACA GAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237476 representing NM_001282169
Red=Cloning site Green=Tags(s) MAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQ RRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSI ANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTE VYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLT SEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001282169 |
ORF Size | 984 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282169.1, NP_001269098.1 |
RefSeq Size | 4607 bp |
RefSeq ORF | 987 bp |
Locus ID | 51028 |
UniProt ID | Q86VN1 |
Protein Families | Transcription Factors |
Protein Pathways | Endocytosis |
MW | 37.4 kDa |
Gene Summary | This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013] |
Documents
Product Manuals |
FAQs |
SDS |