ST13 (NM_001278589) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AAG2; FAM10A1; FAM10A4; HIP; HOP; HSPABP; HSPABP1; P48; PRO0786; SNC6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237711 representing NM_001278589 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCCCGCAAAGTGAACGAGCTTCGGGCCTTTGTGAAAATGTGTAAGCAGGATCCGAGCGTTCTGC ACACCGAGGAAATGCGCTTCCTGAGGGAGTGGGTGGAGAGCATGGGTGGTAAAGTACCACCTGCTACTCA GAAAGCTAAATCAGAAGAAAATACCAAGGAAGACTTAAAGGCAGACGAACCATCAAGTGAGGAAAGTGAT CTAGAAATTGATAAAGAAGGTGTGATTGAACCAGACACTGATGCTCCTCAAGAAATGGGAGATGAAAATG CGGAGATAACGGAGGAGATGATGGATCAGGCAAATGATAAAAAAGTGGCTGCTATTGAAGCCCTAAATGA TGGTGAACTCCAGAAAGCCATTGACTTATTCACAGATGCCATCAAGCTGAATCCTCGCTTGGCCATTTTG TATGCCAAGAGGGCCAGTGTCTTCGTCAAATTACAGAAGCCAAATGCTGCCATCCGAGACTGTGACAGAG CCATTGAAATAAATCCTGATTCAGCTCAGCCTTACAAGTGGCGGGGGAAAGCACACAGACTTCTAGGCCA CTGGGAAGAAGCAGCCCATGATCTTGCCCTTGCCTGTAAATTGGATTATGATGAAGATGCTAGTGCAATG CTGAAAGAAGTTCAACCTAGGGCACAGAAAATTGCAGAACATCGGAGAAAGTATGAGCGAAAACGTGAAG AGCGAGAGATCAAAGAAAGAATAGAACGAGTTAAGAAGGCTCGAGAAGAGCATGAGAGAGCCCAGAGGGA GGAAGAAGCCAGACGACAGTCAGGAGCTCAGTATGGCTCTTTTCCAGGTGGCTTTCCTGGGGGAATGCCT GGTAATTTTCCCGGAGGAATGCCTGGAATGGGAGGGGGCATGCCTGGAATGGCTGGAATGCCTGGACTCA ATGAAATTCTTAGTGATCCAGAGGTTCTTGCAGCCATGCAGGATCCAGAAGTTATGGTGGCTTTCCAGGA TGTGGCTCAGAACCCAGCAAATATGTCAAAATACCAGAGCAACCCAAAGGTTATGAATCTCATCAGTAAA TTGTCAGCCAAATTTGGAGGTCAAGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237711 representing NM_001278589 Red=Cloning site Green=Tags(s) MDPRKVNELRAFVKMCKQDPSVLHTEEMRFLREWVESMGGKVPPATQKAKSEENTKEDLKADEPSSEESD LEIDKEGVIEPDTDAPQEMGDENAEITEEMMDQANDKKVAAIEALNDGELQKAIDLFTDAIKLNPRLAIL YAKRASVFVKLQKPNAAIRDCDRAIEINPDSAQPYKWRGKAHRLLGHWEEAAHDLALACKLDYDEDASAM LKEVQPRAQKIAEHRRKYERKREEREIKERIERVKKAREEHERAQREEEARRQSGAQYGSFPGGFPGGMP GNFPGGMPGMGGGMPGMAGMPGLNEILSDPEVLAAMQDPEVMVAFQDVAQNPANMSKYQSNPKVMNLISK LSAKFGGQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278589
ORF Size	1077 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278589.1, NP_001265518.1
RefSeq Size	3567 bp
RefSeq ORF	1080 bp
Locus ID	6767
Protein Families	Druggable Genome
MW	40.6 kDa
Gene Summary	The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that it is a candidate tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

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