MSL3L1 (MSL3) (NM_001282174) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MRSXBA; MRXS36; MRXSBA; MSL3L1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237808 representing NM_001282174 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATGATGAAAACTAAAGAAGAACCAGAGCTTCAAACAAGAAGGGAAATGGAAGAAAGAACAATAA CTATAGAAATCCCTGAAGTTCTGAAGAAGCAGCTGGAGGATGATTGTTACTACATTAACAGGAGGAAACG GTTAGTGAAACTTCCATGCCAGACCAACATCATAACGATTTTGGAATCCTATGTGAAGCATTTTGCTATC AATGCAGCCTTTTCAGCCAATGAGAGGCCTCGTCACCATCACGTTATGCCACATGCCAACATGAACGTGC ATTATATCCCAGCAGAAAAGAATGTTGACCTTTGTAAGGAGATGGTGGATGGATTAAGAATAACCTTTGA TTACACTCTCCCGTTGGTTTTACTCTATCCATATGAACAAGCTCAGTATAAAAAGGTGACTTCGTCTAAA TTTTTTCTTCCAATTAAGGAAAGTGCCACAAGCACTAACAGGAGCCAGGAGGAACTCTCTCCCAGTCCGC CTTTGTTGAATCCATCCACGCCACAGTCCACAGAGAGTCAGCCGACCACCGGTGAACCAGCCACCCCCAA AAGGCGCAAAGCTGAGCCAGAAGCATTGCAGTCTCTGAGGCGGTCCACGCGCCACAGTGCCAACTGTGAC AGGCTTTCTGAGAGCAGCGCTTCACCTCAGCCCAAGCGCCGGCAGCAGGACACATCCGCCAGCATGCCCA AGCTCTTCCTGCACCTGGAAAAGAAGACACCTGTGCATAGCAGATCATCTTCACCTATTCCTCTGACTCC TAGCAAGGAAGGGAGTGCTGTGTTTGCTGGCTTTGAAGGGAGAAGAACTAATGAAATAAACGAGGTCCTC TCCTGGAAGCTTGTGCCTGACAATTACCCCCCAGGTGACCAGCCGCCTCCACCCTCTTACATTTATGGGG CACAACATTTGCTGCGATTGTTTGTGAAACTTCCAGAAATCCTTGGAAAGATGTCCTTTTCTGAGAAGAA TCTGAAGGCTTTATTGAAGCACTTTGATCTCTTTTTGAGGTTTTTAGCAGAATACCACGATGACTTCTTC CCAGAGTCGGCTTATGTCGCTGCCTGTGAGGCACATTACAGCACCAAGAACCCCCGGGCAATTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237808 representing NM_001282174 Red=Cloning site Green=Tags(s) MKMMKTKEEPELQTRREMEERTITIEIPEVLKKQLEDDCYYINRRKRLVKLPCQTNIITILESYVKHFAI NAAFSANERPRHHHVMPHANMNVHYIPAEKNVDLCKEMVDGLRITFDYTLPLVLLYPYEQAQYKKVTSSK FFLPIKESATSTNRSQEELSPSPPLLNPSTPQSTESQPTTGEPATPKRRKAEPEALQSLRRSTRHSANCD RLSESSASPQPKRRQQDTSASMPKLFLHLEKKTPVHSRSSSPIPLTPSKEGSAVFAGFEGRRTNEINEVL SWKLVPDNYPPGDQPPPPSYIYGAQHLLRLFVKLPEILGKMSFSEKNLKALLKHFDLFLRFLAEYHDDFF PESAYVAACEAHYSTKNPRAIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282174
ORF Size	1116 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282174.1, NP_001269103.1
RefSeq Size	2276 bp
RefSeq ORF	1119 bp
Locus ID	10943
UniProt ID	Q8N5Y2
Protein Families	Transcription Factors
MW	43.2 kDa
Gene Summary	This gene encodes a nuclear protein that is similar to the product of the Drosophila male-specific lethal-3 gene. The Drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus, the human protein is thought to play a similar function in chromatin remodeling and transcriptional regulation, and it has been found as part of a complex that is responsible for histone H4 lysine-16 acetylation. This gene can undergo X inactivation. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 7 and 8. [provided by RefSeq, Jul 2010]

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