GPR6 (NM_001286099) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237844 representing NM_001286099 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCTAGCTTGGTGCACTCGGGGTGCAAATCCGGCCGCGATGAACGCGAGCGCCGCCTCGCTCA ACGACTCCCAGGTGGTGGTAGTGGCGGCCGAAGGAGCGGCGGCGGCGGCCACAGCAGCAGGGGGGCCGGA CACGGGCGAATGGGGACCCCCTGCTGCGGCGGCTCTAGGAGCCGGCGGCGGAGCTAATGGGTCTCTGGAG CTGTCCTCGCAGCTGTCGGCTGGGCCACCGGGACTCCTGCTGCCAGCGGTGAATCCGTGGGACGTGCTCC TGTGCGTGTCGGGGACAGTGATCGCTGGAGAAAACGCGCTGGTGGTGGCGCTCATCGCGTCCACTCCGGC GCTGCGCACGCCCATGTTCGTGCTGGTAGGCAGCCTGGCCACCGCTGACCTGTTGGCGGGCTGTGGCCTC ATCTTGCACTTTGTGTTCCAGTACTTGGTGCCCTCGGAGACTGTGAGTCTGCTCACGGTGGGCTTCCTCG TGGCCTCCTTCGCCGCCTCTGTCAGCAGCCTGCTGGCCATTACGGTGGACCGCTACCTGTCCCTGTATAA CGCGCTCACCTATTACTCGCGCCGGACCCTGTTGGGCGTGCACCTCCTGCTTGCCGCCACTTGGACCGTG TCCCTAGGCCTGGGGCTGCTGCCCGTGCTGGGCTGGAACTGCCTGGCAGAGCGCGCCGCCTGCAGCGTGG TGCGCCCGCTGGCGCGCAGCCACGTGGCTCTGCTCTCCGCCGCCTTCTTCATGGTCTTCGGCATCATGCT GCACCTGTACGTGCGCATCTGCCAGGTGGTCTGGCGCCACGCGCACCAGATCGCGCTGCAGCAGCACTGC CTGGCGCCACCCCATCTCGCTGCCACCAGAAAGGGTGTGGGTACACTGGCTGTGGTGCTGGGCACTTTCG GCGCCAGCTGGCTGCCCTTCGCCATCTATTGCGTGGTGGGCAGCCATGAGGACCCGGCGGTCTACACTTA CGCCACCCTGCTGCCCGCCACCTACAACTCCATGATCAATCCCATCATCTATGCCTTCCGCAACCAGGAG ATCCAGCGCGCCCTGTGGCTCCTGCTCTGTGGCTGTTTCCAGTCCAAAGTGCCCTTTCGTTCCAGGTCTC CCAGCGAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237844 representing NM_001286099 Red=Cloning site Green=Tags(s) MTLLAWCTRGANPAAMNASAASLNDSQVVVVAAEGAAAAATAAGGPDTGEWGPPAAAALGAGGGANGSLE LSSQLSAGPPGLLLPAVNPWDVLLCVSGTVIAGENALVVALIASTPALRTPMFVLVGSLATADLLAGCGL ILHFVFQYLVPSETVSLLTVGFLVASFAASVSSLLAITVDRYLSLYNALTYYSRRTLLGVHLLLAATWTV SLGLGLLPVLGWNCLAERAACSVVRPLARSHVALLSAAFFMVFGIMLHLYVRICQVVWRHAHQIALQQHC LAPPHLAATRKGVGTLAVVLGTFGASWLPFAIYCVVGSHEDPAVYTYATLLPATYNSMINPIIYAFRNQE IQRALWLLLCGCFQSKVPFRSRSPSEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286099
ORF Size	1131 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286099.1, NP_001273028.1
RefSeq Size	1966 bp
RefSeq ORF	1134 bp
Locus ID	2830
UniProt ID	P46095
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	39.9 kDa
Gene Summary	Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity).[UniProtKB/Swiss-Prot Function]

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