RMND5B (NM_001288795) Human Tagged ORF Clone
CAT#: RC237861
- TrueORF®
RMND5B (myc-DDK-tagged) - Human required for meiotic nuclear division 5 homolog B (S. cerevisiae) (RMND5B), transcript variant 3
ORF Plasmid: tGFP
"NM_001288795" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GID2; GID2B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237861 representing NM_001288795
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACTGCAGCCTTGACCTCCCGGGCTCAAGCAGTCCTCCGTCCCACCTCAGCCTTCTGAGGAGCT GGGACCACAGGCGTGTGCCACCATGCCCAGCCCTCCAGGGGACCCCTCTCTCAGCCACCCTCTCTCTGGT GATGTCACAGTGCTGCCGGAAGATCAAAGATACGGTGCAGAAACTGGCTTCGGACCATAAGGACATTCAC AGCAGTGTATCCCGAGTGGGCAAAGCCATTGACAGGAACTTCGACTCTGAGATCTGTGGTGTTGTGTCAG ATGCGGTGTGGGACGCGCGGGAACAGCAGCAGCAGATCCTGCAGATGGCCATCGTGGAACACCTGTATCA GCAGGGCATGCTCAGCGTGGCCGAGGAGCTGTGCCAGGAATCAACGCTGAATGTGGACTTGGATTTCAAG CAGCCTTTCCTAGAGTTGAATCGAATCCTGGAAGCCCTGCACGAACAAGACCTGGGTCCTGCGTTGGAAT GGGCCGTCTCCCACAGGCAGCGCCTGCTGGAACTCAACAGCTCCCTGGAGTTCAAGCTGCACCGACTGCA CTTCATCCGCCTCTTGGCAGGAGGCCCCGCGAAGCAGCTGGAGGCCCTCAGCTATGCTCGGCACTTCCAG CCCTTTGCTCGGCTGCACCAGCGGGAGATCCAGGTGATGATGGGCAGCCTGGTGTACCTGCGGCTGGGCT TGGAGAAGTCACCCTACTGCCACCTGCTGGACAGCAGCCACTGGGCAGAGATCTGTGAGACCTTTACCCG GGACGCCTGTTCCCTGCTGGGGCTTTCTGTGGAGTCCCCCCTTAGCGTCAGCTTTGCCTCTGGCTGTGTG GCGCTGCCTGTGTTGATGAACATCAAGGCTGTGATTGAGCAGCGGCAGTGCACTGGGGTCTGGAATCACA AGGACGAGTTACCGATTGAGATTGAACTAGGCATGAAGTGCTGGTACCACTCCGTGTTCGCTTGCCCCAT CCTCCGCCAGCAGACGTCAGATTCCAACCCTCCCATCAAGCTCATCTGTGGCCATGTTATCTCCCGAGAT GCACTCAATAAGCTCATTAATGGAGGAAAGCTGAAGTGTCCCTACTGTCCCATGGAGCAGAACCCGGCAG ATGGGAAACGCATCATATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237861 representing NM_001288795
Red=Cloning site Green=Tags(s) MADCSLDLPGSSSPPSHLSLLRSWDHRRVPPCPALQGTPLSATLSLVMSQCCRKIKDTVQKLASDHKDIH SSVSRVGKAIDRNFDSEICGVVSDAVWDAREQQQQILQMAIVEHLYQQGMLSVAEELCQESTLNVDLDFK QPFLELNRILEALHEQDLGPALEWAVSHRQRLLELNSSLEFKLHRLHFIRLLAGGPAKQLEALSYARHFQ PFARLHQREIQVMMGSLVYLRLGLEKSPYCHLLDSSHWAEICETFTRDACSLLGLSVESPLSVSFASGCV ALPVLMNIKAVIEQRQCTGVWNHKDELPIEIELGMKCWYHSVFACPILRQQTSDSNPPIKLICGHVISRD ALNKLINGGKLKCPYCPMEQNPADGKRIIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001288795 |
ORF Size | 1140 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001288795.1, NP_001275724.1 |
RefSeq Size | 1973 bp |
RefSeq ORF | 1143 bp |
Locus ID | 64777 |
UniProt ID | Q96G75 |
Protein Families | Stem cell - Pluripotency |
MW | 43.1 kDa |
Gene Summary | Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972). Catalytic activity of the complex is required for normal cell proliferation (PubMed:29911972). The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (PubMed:29911972).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG237861 | RMND5B (tGFP-tagged) - Human required for meiotic nuclear division 5 homolog B (S. cerevisiae) (RMND5B), transcript variant 3 |
CNY 4,370.00 |
|
SC335755 | RMND5B (untagged) - Human required for meiotic nuclear division 5 homolog B (S. cerevisiae) (RMND5B), transcript variant 3 |
CNY 3,610.00 |