Myotilin (MYOT) (NM_001300911) Human Tagged ORF Clone
CAT#: RC237870
- TrueORF®
MYOT (myc-DDK-tagged) - Human myotilin (MYOT), transcript variant 3
ORF Plasmid: tGFP
"NM_001300911" in other vectors (2)
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CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | LGMD1; LGMD1A; MFM3; TTID; TTOD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237870 representing NM_001300911
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCAACTATCAACAGTCCTCAGCTGGCCAACCTATAAATGCAAAGCCATCCCAAACTGCAAATG CTAAGCCCATACCAAGAACTCCTGATCATGAAATACAAGGATCAAAAGAAGCTTTGATTCAAGATTTGGA AAGAAAGCTGAAATGCAAGGACACCCTTCTTCATAATGGAAATCAACGTCTAACATATGAAGAGAAGATG GCTCGCAGATTGCTAGGACCACAGAATGCAGCTGCTGTGTTTCAAGCTCAGGATGACAGTGGTGCACAAG ACTCGCAGCAACACAACTCAGAACATGCGCGACTGCAAGTTCCTACATCACAAGTAAGAAGTAGATCAAC CTCAAGGGGAGATGTGAATGATCAGGATGCAATCCAGGAGAAATTTTACCCACCACGTTTCATTCAAGTG CCAGAGAACATGTCGATTGATGAAGGAAGATTCTGCAGAATGGACTTCAAAGTGAGTGGACTGCCAGCTC CTGATGTGTCATGGTATCTAAATGGAAGAACAGTTCAATCAGATGATTTGCACAAAATGATAGTGTCTGA GAAGGGTCTTCATTCACTCATCTTTGAAGTAGTCAGAGCTTCAGATGCAGGGGCTTATGCATGTGTTGCC AAGAATAGAGCAGGAGAAGCCACCTTCACTGTGCAGCTGGATGTCCTTGCAAAAGAACATAAAAGAGCAC CAATGTTTATCTACAAACCACAGAGCAAAAAAGTTTTAGAGGGAGATTCAGTGAAACTAGAATGCCAGAT CTCGGCTATACCTCCACCAAAGCTTTTCTGGAAAAGAAATAATGAAATGGTACAATTCAACACTGACCGA ATAAGCTTATATCAAGATAACACTGGAAGAGTTACTTTACTGATAAAAGATGTAAACAAGAAAGATGCTG GGTGGTATACTGTGTCAGCAGTTAATGAAGCTGGAGTGACTACATGTAACACAAGATTAGACGTTACGGC ACGTCCAAACCAAACTCTTCCAGCTCCTAAGCAGTTACGGGTTCGACCAACATTCAGCAAATATTTAGCA CTTAATGGGAAAGGTTTGAATGTAAAACAAGCTTTTAACCCAGAAGGAGAATTTCAGCGTTTGGCAGCTC AATCTGGACTCTATGAAAGTGAAGAACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237870 representing NM_001300911
Red=Cloning site Green=Tags(s) MDSNYQQSSAGQPINAKPSQTANAKPIPRTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKM ARRLLGPQNAAAVFQAQDDSGAQDSQQHNSEHARLQVPTSQVRSRSTSRGDVNDQDAIQEKFYPPRFIQV PENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIVSEKGLHSLIFEVVRASDAGAYACVA KNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKVLEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDR ISLYQDNTGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLA LNGKGLNVKQAFNPEGEFQRLAAQSGLYESEEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001300911 |
ORF Size | 1149 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001300911.2 |
RefSeq Size | 1939 bp |
RefSeq ORF | 1152 bp |
Locus ID | 9499 |
UniProt ID | Q9UBF9 |
MW | 43.4 kDa |
Gene Summary | This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008] |
Documents
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