TOM1L2 (NM_001288788) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237914 representing NM_001288788 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCTCCTGGGGAACCCGTTCAGCACACCAGTGGGGCAGTGCCTCGAAAAGGCAACAGATGGCT CCCTGCAAAGTGAGGATTGGACGTTGAATATGGAGATCTGTGACATCATCAATGAGACGGAGGAAGGGCC AAAGGATGCCATTCGAGCCCTGAAGAAGCGGCTCAACGGGAACCGGAACTACAGAGAGGTGATGCTGGCA TTAACAGTGCTGGAGACATGTGTGAAGAACTGTGGCCACCGCTTCCACATCCTTGTGGCCAACCGAGATT TCATCGACAGTGTTCTGGTCAAAATTATATCTCCCAAGAACAACCCTCCCACCATTGTACAGGACAAAGT GCTTGCTCTGATCCAGATTGCCAGGCTGCGGAGTGAACTGGACGTCGTTCGAGGAAACACAAAAGTCATG TCTGAGATGTTAACAGAAATGGTCCCTGGACAGGAGGATTCATCTGATCTGGAGTTGCTGCAGGAGCTCA ACAGGACCTGTCGGGCCATGCAGCAGCGCATCGTGGAGCTCATCTCCCGCGTGTCCAATGAGGAGGTCAC CGAGGAGCTGCTGCATGTGAACGATGACCTCAACAACGTCTTCCTTCGATACGAGAGGTTCGAACGATAC AGGTCTGGCCGATCCGTTCAAAATGCCAGTAATGGAGTACTGAATGAAGTAACCGAAGACAACTTAATAG ACCTGGGGCCAGGGTCTCCAGCCGTGGTGAGCCCAATGGTGGGGAACACAGCGCCCCCATCTTCCCTCTC CTCCCAGCTTGCAGGCTTAGACTTGGGGACAGAGAGCGTCAGTGGCACCCTCAGTTCACTCCAGCAATGT AATCCCCGTGACGGCTTTGACATGTTTGCCCAGACGAGAGGAAACTCCTTGGCTGAGCAGCGCAAGACGG TAACCTATGAGGATCCTCAGGCTGTCGGAGGACTTGCTTCTGCACTAGACAATCGAAAACAGAGTTCAGA AGGGAAGGGTGATGATCTGGAGGAGGGTGTCACAAGTGAAGAGTTTGATAAATTCCTTGAAGAAAGAGCC AAAGCTGCTGAAATGGTTCCCGACCTCCCCTCGCCCCCCATGGAGGCTCCTGCCCCAGCCTCAAACCCTT CTGGCCGGAAGAAGCCAGAGCGGTCAGAGGATGCCCTCTTCGCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237914 representing NM_001288788 Red=Cloning site Green=Tags(s) MEFLLGNPFSTPVGQCLEKATDGSLQSEDWTLNMEICDIINETEEGPKDAIRALKKRLNGNRNYREVMLA LTVLETCVKNCGHRFHILVANRDFIDSVLVKIISPKNNPPTIVQDKVLALIQIARLRSELDVVRGNTKVM SEMLTEMVPGQEDSSDLELLQELNRTCRAMQQRIVELISRVSNEEVTEELLHVNDDLNNVFLRYERFERY RSGRSVQNASNGVLNEVTEDNLIDLGPGSPAVVSPMVGNTAPPSSLSSQLAGLDLGTESVSGTLSSLQQC NPRDGFDMFAQTRGNSLAEQRKTVTYEDPQAVGGLASALDNRKQSSEGKGDDLEEGVTSEEFDKFLEERA KAAEMVPDLPSPPMEAPAPASNPSGRKKPERSEDALFAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288788
ORF Size	1167 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288788.2
RefSeq Size	5471 bp
RefSeq ORF	1170 bp
Locus ID	146691
UniProt ID	Q6ZVM7
MW	43.3 kDa
Gene Summary	This gene belongs to a small gene family whose members have an N-terminal VHS domain followed by a GAT domain; domains which typically participate in vesicular trafficking. The canonical protein encoded by this gene also has a C-terminal clathrin binding motif. This protein has been shown to interact with Tollip, clathrin and ubiquitin and is thought to play a role in endosomal sorting. This gene resides in the 3.7 Mb deletion of chromosome region 17p11.2 that is associated with Smith-Magenis syndrome. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Apr 2017]

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