HHLA2 (NM_001282558) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	B7-H5; B7-H7; B7H7; B7y
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237962 representing NM_001282558 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCACAGACAGCACTGTCTTTCTTCCTCATTCTCATAACATCTCTGAGTGGATCTCAAGGCATAT TCCCTTTGGCTTTCTTCATTTATGTTCCTATGAATGAACAAATCGTCATTGGAAGACTTGATGAAGATAT AATTCTCCCTTCTTCATTTGAGAGGGGATCCGAAGTCGTAATACACTGGAAGTATCAAGATAGCTATAAG GTTCACAGTTACTACAAAGGCAGTGACCATTTGGAAAGCCAAGATCCCAGATATGCAAACAGGACATCCC TTTTCTATAATGAGATTCAAAATGGGAATGCGTCGCTATTTTTCAGAAGAGTAAGCCTTCTGGACGAAGG AATTTACACCTGCTATGTAGGAACAGCAATTCAAGTGATTACAAACAAAGTGGTGCTAAAGGTGGGAGTT TTTCTCACACCCGTGATGAAGTATGAAAAGAGGAACACAAACAGCTTCTTAATATGCAGCGTGTTAAGTG TTTATCCTCGTCCAATTATCACGTGGAAAATGGACAACACACCTATCTCTGAAAACAACATGGAAGAAAC AGGGTCTTTGGATTCTTTTTCTATTAACAGCCCACTGAATATTACAGGATCAAATTCATCTTATGAATGT ACAATTGAAAATTCACTGCTGAAGCAAACATGGACAGGGCGCTGGACGATGAAAGATGGCCTTCATAAAA TGCAAAGTGAACACGTTTCACTCTCATGTCAACCTGTAAATGATTATTTTTCACCAAACCAAGACTTCAA AGTTACTTGGTCCAGAATGAAAAGTGGGACTTTCTCTGTCCTGGCTTACTATCTGAGCTCCTCACAAAAT ACAATTATCAATGAATCCCGATTCTCATGGAACAAAGAGCTGATAAACCAGAGTGACTTCTCTATGAATT TGATGGATCTTAATCTTTCAGACAGTGGGGAATATTTATGCAATATTTCTTCGGATGAATATACTTTACT TACCATCCACACAGTGCATGTAGAACCGAGCCAAGAAACAGCTTCCCATAACAAAGGCTTATGGATTTTG GTGCCCTCTGCGATTTTGGCAGCTTTTCTGCTGATTTGGAGCGTAAAATGTTGCAGAGAAAGATGTTGTG TCCCTCCTGGTGAGCGCTGTCCCAGTGCACCCGATAATGGCGAAGAAAATGTGCCTCTTTCAGGAAAAGT A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237962 representing NM_001282558 Red=Cloning site Green=Tags(s) MKAQTALSFFLILITSLSGSQGIFPLAFFIYVPMNEQIVIGRLDEDIILPSSFERGSEVVIHWKYQDSYK VHSYYKGSDHLESQDPRYANRTSLFYNEIQNGNASLFFRRVSLLDEGIYTCYVGTAIQVITNKVVLKVGV FLTPVMKYEKRNTNSFLICSVLSVYPRPIITWKMDNTPISENNMEETGSLDSFSINSPLNITGSNSSYEC TIENSLLKQTWTGRWTMKDGLHKMQSEHVSLSCQPVNDYFSPNQDFKVTWSRMKSGTFSVLAYYLSSSQN TIINESRFSWNKELINQSDFSMNLMDLNLSDSGEYLCNISSDEYTLLTIHTVHVEPSQETASHNKGLWIL VPSAILAAFLLIWSVKCCRERCCVPPGERCPSAPDNGEENVPLSGKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282558
ORF Size	1191 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282558.1, NP_001269487.1
RefSeq Size	2515 bp
RefSeq ORF	1194 bp
Locus ID	11148
UniProt ID	Q9UM44
Protein Families	Transmembrane
MW	45.4 kDa
Gene Summary	This gene encodes a protein ligand found on the surface of monocytes. The encoded protein is thought to regulate cell-mediated immunity by binding to a receptor on T lymphocytes and inhibiting the proliferation of these cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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