ACSF2 (NM_001288972) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ACSMW; AVYV493
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238326 representing NM_001288972 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTGGAGTATGTCCTCAAGAAGGTGGGCTGCAAGGCCCTTGTGTTCCCCAAGCAATTCAAGACCC AGCAATACTACAACGTCCTGAAGCAGATCTGTCCAGAAGTGGAGAATGCCCAGCCAGGGGCCTTGAAGAG TCAGAGGCTCCCAGATCTGACCACAGTCATCTCGGTGGATGCCCCTTTGCCGGGGACCCTGCTCCTGGAT GAAGTGGTGGCGGCTGGCAGCACACGGCAGCATCTGGACCAGCTCCAATACAACCAGCAGTTCCTGTCCT GCCATGACCCCATCAACATCCAGTTCACCTCGGGGACAACAGGCAGCCCCAAGGGGGCCACCCTCTCCCA CTACAACATTGTCAACAACTCCAACATTTTAGGAGAGCGCCTGAAACTGCATGAGAAGACACCAGAGCAG TTGCGGATGATCCTGCCCAACCCCCTGTACCATTGCCTGGGTTCCGTGGCAGGCACAATGATGTGTCTGA TGTACGGTGCCACCCTCATCCTGGCCTCTCCCATCTTCAATGGCAAGAAGGCACTGGAGGCCATCAGCAG AGAGAGAGGCACCTTCCTGTATGGTACCCCCACGATGTTCGTGGACATTCTGAACCAGCCAGACTTCTCC AGTTATGACATCTCGACCATGTGTGGAGGTGTCATTGCTGGGTCCCCTGCACCTCCAGAGTTGATCCGAG CCATCATCAACAAGATAAATATGAAGGACCTGGTGGTTGCTTATGGAACCACAGAGAACAGTCCCGTGAC ATTCGCGCACTTCCCTGAGGACACTGTGGAGCAGAAGGCAGAAAGCGTGGGCAGAATTATGCCTCACACG GAGGCCCGGATCATGAACATGGAGGCAGGGACGCTGGCAAAGCTGAACACGCCCGGGGAGCTGTGCATCC GAGGGTACTGCGTCATGCTGGGCTACTGGGGTGAGCCTCAGAAGACAGAGGAAGCAGTGGATCAGGACAA GTGGTATTGGACAGGAGATGTCGCCACAATGAATGAGCAGGGCTTCTGCAAGATCGTGGGCCGCTCTAAG GATATGATCATCCGGGGTGGTGAGAACATCTACCCCGCAGAGCTCGAGGACTTCTTTCACACACACCCGA AGGTGCAGGAAGTGCAGGTGGTGGGAGTGAAGGACGATCGGATGGGGGAAGAGATTTGTGCCTGCATTCG GCTGAAGGACGGGGAGGAGACCACGGTGGAGGAGATAAAAGCTTTCTGCAAAGGGAAGATCTCTCACTTC AAGATTCCGAAGTACATCGTGTTTGTCACAAACTACCCCCTCACCATTTCAGGAAAGATCCAGAAATTCA AACTTCGAGAGCAGATGGAACGACATCTAAATCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238326 representing NM_001288972 Red=Cloning site Green=Tags(s) MELEYVLKKVGCKALVFPKQFKTQQYYNVLKQICPEVENAQPGALKSQRLPDLTTVISVDAPLPGTLLLD EVVAAGSTRQHLDQLQYNQQFLSCHDPINIQFTSGTTGSPKGATLSHYNIVNNSNILGERLKLHEKTPEQ LRMILPNPLYHCLGSVAGTMMCLMYGATLILASPIFNGKKALEAISRERGTFLYGTPTMFVDILNQPDFS SYDISTMCGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPVTFAHFPEDTVEQKAESVGRIMPHT EARIMNMEAGTLAKLNTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVATMNEQGFCKIVGRSK DMIIRGGENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTVEEIKAFCKGKISHF KIPKYIVFVTNYPLTISGKIQKFKLREQMERHLNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288972
ORF Size	1365 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288972.1, NP_001275901.1
RefSeq Size	2162 bp
RefSeq ORF	1368 bp
Locus ID	80221
UniProt ID	Q96CM8
MW	51.3 kDa
Gene Summary	Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation.[UniProtKB/Swiss-Prot Function]

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