C3IP1 (KLHL12) (NM_001303109) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C3IP1; DKIR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238398 representing NM_001303109 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATTCAATGA ACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGAAAGACTTCCCTGCCCA TCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGTTCACTAGTGAGCTCTCAGAGAAGGGG AAACCTTATGTTGACATCCAAGGTTTGACTGCCTCTACCATGGAAATTTTATTGGACTTTGTGTACACAG AAACAGTACATGTGACAGTGGAGAATGTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGG TGTGAAACAAGCCTGCTGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGAT TTTGCTGAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCATTTTCCTG AAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAAAAGCTAATCAAGTGCGACGA AATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTCATCAACTGGGTGAAGCATGCCAAGAAAGAG CGGGAAGAATCCTTGCCTAACCTGCTACAGTATGTGCGGATGCCCCTACTAACCCCCAGGTATATCACAG ATGTAATAGATGCTGAGCCTTTCATCCGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAA GTTTCATCTGAGGCCTGAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGATATGATC TATGTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGATCCAAACATTGACC AGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGACTCGTAGTGGCCAGTGGAGTGAT CTACTGTCTAGGAGGATATGACGGCTTGAATATCTTAAATTCAGTTGAGAAATACGACCCTCATACAGGA CATTGGACTAATGTTACACCAATGGCCACCAAGCGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATA TTTATGTGGTGGGGGGATTTGATGGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGA TTCCTGGACAACTGTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGGAGA CTCTATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTATGACCCTATCATCG ACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCTGGTGTTTGTGTTCTCCGCGAGAA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238398 representing NM_001303109 Red=Cloning site Green=Tags(s) MGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDVTLRVEQKDFPAHRIVLAACSDYFCAMFTSELSEKG KPYVDIQGLTASTMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRD FAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKE REESLPNLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLDMI YVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGVIYCLGGYDGLNILNSVEKYDPHTG HWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGR LYAIAGYDGNSLLSSIECYDPIIDSWEVVTSMGTQRCDAGVCVLREK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303109
ORF Size	1401 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303109.2
RefSeq Size	3081 bp
RefSeq ORF	1404 bp
Locus ID	59349
UniProt ID	Q53G59
MW	52.6 kDa
Gene Summary	This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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