KLHL32 (NM_001286252) Human Tagged ORF Clone
CAT#: RC238418
- TrueORF®
KLHL32 (myc-DDK-tagged) - Human kelch-like family member 32 (KLHL32), transcript variant 4
ORF Plasmid: tGFP
"NM_001286252" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BKLHD5; dJ21F7.1; KIAA1900; UG0030H05 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC238418 representing NM_001286252
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTCTGAACGCTGCCTCAGTATTCAAGAAATGCTGACAGGCCAGAGGCTCTGCCACTCCGAATCTC ACAATGACAGTGTCCTGGCAGCGCTGAATCAGCAGAGGAGTGATGGCATCCTCTGCGACATCACCCTGAT TGCTGAGGAACAGAAATTCCATGCTCACAAGGCAGTCCTAGCAGCATGCAGTGACTATTTCCGGGCAATG TTCAGTCTTTGTATGGTGGAAAGTGGAGCTGATGAGGTTAATTTGCACGGTGTGACCAGCCTTGGCTTAA AGCAGGCTCTGGAGTTTGCATACACAGGACAGATTTTGCTGGAGCCAGGTGTGATCCAGGATGTGCTAGC AGCGGGCAGTCACCTACAGCTGTTGGAGCTTCTCAATTTATGCTCCCACTATCTCATCCAGGAATTAAAT AGCTTTAATTACTTGGATCTGTACAGACTTGCTGACCTCTTTAACCTCACTTTGTTGGAGAAGGCAGTGA TCGATTTCTTAGTGAAACATCTCTCTGAACTCCTGAAGAGCCGCCCAGAAGAAGTTCTAACGCTTCCCTA TTGCCTGCTTCAGGAGGTGCTGAAGAGCGACCGCCTGACCTCCCTGAGTGAAGAGCAGATCTGGCAGCTA GCTGTGAGGTGGTTGGAACACAACTGCCACTACCAGTACATGGACGAGCTCCTGCAATACATCCGCTTTG GCCTAATGGATGTGGATACTCTCCATACAGTTGCCCTGTCCCACCCCCTTGTCCAAGCAAGTGAGACTGC AACAGCCCTTGTCAACGAGGCCCTGGAATACCACCAGAGCATCTATGCACAGCCTGTCTGGCAGACTCGC AGGACCAAACCACGATTCCAGTCAGACACTCTGTATATCATTGGTGGGAAAAAGCGCGAGGTCTGCAAGG TCAAGGAACTTCGGTACTTCAATCCTGTTGATCAGGAGAATGCTCTCATAGCTGCCATTGCCAACTGGAG TGAGCTGGCTCCCATGCCTGTGGGAAGGAGCCACCATTGTGTGGCAGTCATGGGGGACTTCCTGTTTGTG GCAGGAGGGGAAGTTGAGCATGCCAGTGGCCGGACGTGTGCTGTGAGGACTGCCTGTCGCTATGACCCCC GCAGTAATTCCTGGGCAGAGATAGCACCCATGAAAAACTGCCGGGAGCATTTTGTGCTGGGTGCCATGGA GGAATACCTCTATGCAGTTGGGGGCAGAAATGAACTGCGCCAGGTTCTGCCTACAGTTGAGCGATATTGC CCCAAGAAGAACAAATGGACTTTTGTTCAGTCCTTTGACAGATCCCTTTCATGCCATGCTGGATATGTGG CTGATGGTCTTCTTTGGATATCAGGTAGAACATACCTCATGTTGGATTTATCAAAACACACTTTCATTGT GGTATATATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC238418 representing NM_001286252
Red=Cloning site Green=Tags(s) MPSERCLSIQEMLTGQRLCHSESHNDSVLAALNQQRSDGILCDITLIAEEQKFHAHKAVLAACSDYFRAM FSLCMVESGADEVNLHGVTSLGLKQALEFAYTGQILLEPGVIQDVLAAGSHLQLLELLNLCSHYLIQELN SFNYLDLYRLADLFNLTLLEKAVIDFLVKHLSELLKSRPEEVLTLPYCLLQEVLKSDRLTSLSEEQIWQL AVRWLEHNCHYQYMDELLQYIRFGLMDVDTLHTVALSHPLVQASETATALVNEALEYHQSIYAQPVWQTR RTKPRFQSDTLYIIGGKKREVCKVKELRYFNPVDQENALIAAIANWSELAPMPVGRSHHCVAVMGDFLFV AGGEVEHASGRTCAVRTACRYDPRSNSWAEIAPMKNCREHFVLGAMEEYLYAVGGRNELRQVLPTVERYC PKKNKWTFVQSFDRSLSCHAGYVADGLLWISGRTYLMLDLSKHTFIVVYI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001286252 |
ORF Size | 1410 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286252.2 |
RefSeq Size | 2071 bp |
RefSeq ORF | 1413 bp |
Locus ID | 114792 |
MW | 53.9 kDa |
Documents
Product Manuals |
FAQs |
SDS |